superscript iii rnase h - reverse transcriptase (Promega)
90
Structured Review
Promega
superscript iii rnase h - reverse transcriptase
Superscript Iii Rnase H Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcriptase/superscript+ii+reverse+transcriptase/pmc01626524-68-32-42
Average 90 stars, based on 1 article reviews
Superscript Iii Rnase H Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcriptase/superscript+ii+reverse+transcriptase/pmc01626524-68-32-42
Average 90 stars, based on 1 article reviews
superscript iii rnase h - reverse transcriptase - by Bioz Stars,
2026-09
90/100 stars
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Reverse Transcription:Article Title: Evidence for the involvement of secondary bile acids on peripheral and central regulation of feed intake in rainbow trout. Article Snippet: 20 Recent studies suggest that secondary bile acids (SBAs) play a role in energy metabolism and feed 21 intake regulation, but their effects in fish remain largely unknown.. This study evaluates the impact of 22 intragastric administration of the main SBAs (500 μM lithocholic acid -LCA-, 1500 μM deoxycholic acid 23 DCA-, and their taurine conjugates: 1000 μM T-LCA and 600 μM T-DCA) on feed intake, regulatory 24 pathways, and BA-related elements in rainbow trout.. Results show that all tested SBAs influenced bile 25 acid transporters (Asbt, Ntcp, Ostα, Ostβ) and receptors (Fxrα, Fxrβ, Tgr5), with DCA and T-DCA mainly 26 affecting the gastrointestinal tract and LCA modulating hypothalamic pathways, suggesting a putative 27 orexigenic role. Article Title: SYNLAC prime probiotics enhances growth performance, and resistance of white shrimp, Penaeus vannamei to Enterocytozoon hepatopenaei and Vibrio alginollyticus: Insights into immune and metabolic pathway modulations. Article Snippet: This study explores the impact of SYNLAC Prime probiotics on the growth performance, health status, and metabolic profile of white shrimp, Penaeus vannamei.. Shrimp fed with the experimental diets, including the control diet without probiotic supplementation, and the diets supplemented with SYNLAC Prime probiotics at concentrations of 105 CFU (g diet) 1 (P5) and 106 CFU (g diet) 1 (P6) for 56 days.. Results indicated a significant enhancement in growth performance in probiotic-treated shrimp relative to the control group, attributed to structural improvements in the digestive tract, particularly the increased abundances of B cells in the hepatopancreas. Article Title: The brown fat-specific overexpression of RBP4 improves thermoregulation and systemic metabolism by activating the canonical adrenergic signaling pathway Article Snippet: Protein bands were visualized using an enhanced chemiluminescence kit (Thermo Fisher Scientific), and the polyclonal anti-mouse γ-tubulin antibody (1:5000; Sigma-Aldrich #T6557) was used as a loading control. .. Total RNA was isolated from tissues or cells using TRIzol (Life Technologies, Waltham, MA, USA) and reverse-transcribed to cDNA using Article Title: Stable expression of voltage-gated calcium channel mRNA in α 2 δ (CACNA2D) knockout mouse brains. Article Snippet: After that, total RNA was immediately isolated with the RNeasy Protect Mini Kit following the manufacturer’s instructions (Qiagen, Hilden, Germany) and concentrations were determined using a NanoDrop 2000 spectrophotometer. .. Reverse transcription of 1 μg of RNA was done using Article Title: Mucosal microbiota characterization in gastric cancer identifies immune-activated–related transcripts relevant gastric microbiome signatures Article Snippet: .. Total RNA from GC tumor and paired normal adjacent tissues was isolated using Trizol (Invitrogen, USA). cDNA was synthesized using Article Title: The simultaneous application of fulvic acid and protein hydrolysate biostimulants enhances cucumber responses to Fe deficiency Article Snippet: DNase I treatment was carried out for each sample using one microgram of total RNA and the RQ1 RNase-Free DNase (Promega, Madison, WI, United States) in accordance with the manufacturer’s procedure. .. DNase-treated samples were then used to synthesize cDNA with the Article Title: A circadian clock controls the daily function of the intestine in rainbow trout. Influence of light and food as synchronizers. Article Snippet: Environmental factors (daily light/dark cycles, food availability, etc.) entrain endogenous oscillators in living organisms, thereby allowing them to control the rhythms of behavioral and physiological functions, such as energy homeostasis.. The gastrointestinal tract (GIT) is the first site of nutrient contact upon food intake.. Thus, the GIT is key in energy homeostasis. DNA Synthesis:Article Title: SYNLAC prime probiotics enhances growth performance, and resistance of white shrimp, Penaeus vannamei to Enterocytozoon hepatopenaei and Vibrio alginollyticus: Insights into immune and metabolic pathway modulations. Article Snippet: This study explores the impact of SYNLAC Prime probiotics on the growth performance, health status, and metabolic profile of white shrimp, Penaeus vannamei.. Shrimp fed with the experimental diets, including the control diet without probiotic supplementation, and the diets supplemented with SYNLAC Prime probiotics at concentrations of 105 CFU (g diet) 1 (P5) and 106 CFU (g diet) 1 (P6) for 56 days.. Results indicated a significant enhancement in growth performance in probiotic-treated shrimp relative to the control group, attributed to structural improvements in the digestive tract, particularly the increased abundances of B cells in the hepatopancreas. Isolation:Article Title: The brown fat-specific overexpression of RBP4 improves thermoregulation and systemic metabolism by activating the canonical adrenergic signaling pathway Article Snippet: Protein bands were visualized using an enhanced chemiluminescence kit (Thermo Fisher Scientific), and the polyclonal anti-mouse γ-tubulin antibody (1:5000; Sigma-Aldrich #T6557) was used as a loading control. .. Total RNA was isolated from tissues or cells using TRIzol (Life Technologies, Waltham, MA, USA) and reverse-transcribed to cDNA using Article Title: Mucosal microbiota characterization in gastric cancer identifies immune-activated–related transcripts relevant gastric microbiome signatures Article Snippet: .. Total RNA from GC tumor and paired normal adjacent tissues was isolated using Trizol (Invitrogen, USA). cDNA was synthesized using Random Hexamer:Article Title: Stable expression of voltage-gated calcium channel mRNA in α 2 δ (CACNA2D) knockout mouse brains. Article Snippet: After that, total RNA was immediately isolated with the RNeasy Protect Mini Kit following the manufacturer’s instructions (Qiagen, Hilden, Germany) and concentrations were determined using a NanoDrop 2000 spectrophotometer. .. Reverse transcription of 1 μg of RNA was done using Synthesized:Article Title: Mucosal microbiota characterization in gastric cancer identifies immune-activated–related transcripts relevant gastric microbiome signatures Article Snippet: .. Total RNA from GC tumor and paired normal adjacent tissues was isolated using Trizol (Invitrogen, USA). cDNA was synthesized using other:Article Title: Fermented fish meal derived from barramundi by-product enhances growth, immunity, and gut microbiota in white shrimp Penaeus vannamei Article Snippet: This study investigated the effects of barramundi, Lates calcarifer, by-product fish meals on the growth, immunity, and gut microbiota of white shrimp, Penaeus vannamei.. Three variants were assessed: untreated fish meal (BFM), enzymatically hydrolyzed fish meal (EBFM), and fermented fish meal (FBFM), alongside a commercial fish meal control.. Compared to commercial fish meal, barramundi by-product meals exhibited lower crude protein but higher lipid and ash contents. |