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LeGene Biosciences superscript reverse transcriptase kit
Superscript Reverse Transcriptase Kit, supplied by LeGene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+reverse+transcriptase+kit/superscript+reverse+transcriptase+kit/pmc05173360-62-6-10
Average 90 stars, based on 1 article reviews
superscript reverse transcriptase kit - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Toxic effects of atrazine on porcine oocytes and possible mechanisms of action
Article Snippet: Briefly, mRNA was extracted from 50 MII oocytes or 20 blastocysts by means of the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. The isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA), using oligo(dT) 12–18 primers, followed by real-time PCR on a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Article Title: The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms
Article Snippet: Briefly, 50 MII-stage oocytes or 20 blastocysts were used to extract mRNA using the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. Isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA) using oligo(dT)12–18 primers, followed by real-time PCR in a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Reverse Transcription:

Article Title: Toxic effects of atrazine on porcine oocytes and possible mechanisms of action
Article Snippet: Briefly, mRNA was extracted from 50 MII oocytes or 20 blastocysts by means of the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. The isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA), using oligo(dT) 12–18 primers, followed by real-time PCR on a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Article Title: The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms
Article Snippet: Briefly, 50 MII-stage oocytes or 20 blastocysts were used to extract mRNA using the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. Isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA) using oligo(dT)12–18 primers, followed by real-time PCR in a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Real-time Polymerase Chain Reaction:

Article Title: Toxic effects of atrazine on porcine oocytes and possible mechanisms of action
Article Snippet: Briefly, mRNA was extracted from 50 MII oocytes or 20 blastocysts by means of the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. The isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA), using oligo(dT) 12–18 primers, followed by real-time PCR on a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Article Title: The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms
Article Snippet: Briefly, 50 MII-stage oocytes or 20 blastocysts were used to extract mRNA using the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. Isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA) using oligo(dT)12–18 primers, followed by real-time PCR in a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Polymerase Chain Reaction:

Article Title: Toxic effects of atrazine on porcine oocytes and possible mechanisms of action
Article Snippet: Briefly, mRNA was extracted from 50 MII oocytes or 20 blastocysts by means of the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. The isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA), using oligo(dT) 12–18 primers, followed by real-time PCR on a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..

Article Title: The Role of Glucose Metabolism on Porcine Oocyte Cytoplasmic Maturation and Its Possible Mechanisms
Article Snippet: Briefly, 50 MII-stage oocytes or 20 blastocysts were used to extract mRNA using the Ambion Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). .. Isolated mRNA was reverse-transcribed with the SuperScript Reverse Transcriptase Kit (LeGene Biosciences, San Diego, CA, USA) using oligo(dT)12–18 primers, followed by real-time PCR in a Bio-Rad CFX PCR cycler (Hercules, CA, USA) with the primers that were described in our previous paper [ ]. ..



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