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superscript™ ii rnaseh − reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript™ ii rnaseh − reverse transcriptase kit
    Superscript™ Ii Rnaseh − Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rnaseh+reverse+transcriptase/pmc03398047-159-26-33
    Average 90 stars, based on 1 article reviews
    superscript™ ii rnaseh − reverse transcriptase kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Transcript profiling of candidate genes in testis of pigs exhibiting large differences in androstenone levels
    Article Snippet: First strand cDNA synthesis was conducted using SuperScript-II Rnase H - Reverse Transcriptase (Invitrogen, Carlsbad, CA).

    Article Title: Tyrosine-specific MAPK phosphatases and the control of ERK signaling in PC12 cells
    Article Snippet: Briefly, cDNA was synthesized from total RNA by random hexamer priming using SuperScript II RNase H - Reverse Transcriptase (Invitrogen Life Technologies, Breda, The Netherlands) and used for PCR.

    Article Title: AAV-Dominant Negative Tumor Necrosis Factor (DN-TNF) Gene Transfer to the Striatum Does Not Rescue Medium Spiny Neurons in the YAC128 Mouse Model of Huntington's Disease
    Article Snippet: Left striatum samples (n = 3–5 animals per group) were collected and flash frozen as described above and homogenized in ice-cold RLT buffer (Qiagen, Valencia, CA) containing 2-mercaptoethanol using a rotor-stator homogenizer followed by centrifugation in QIAshredder spin columns (Qiagen).

    Article Title: Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry
    Article Snippet: Hybridization probes were prepared according to Affymetrix instruction [ ].

    Extraction:

    Article Title: Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling
    Article Snippet: .. After extraction with the use of RNeasy Mini Kits (QIAGEN, Valencia, CA), RNA samples were subjected to reverse transcription with SuperScript II RNase H reverse transcriptase (Invitrogen, Carlsbad, CA). .. Realtime quantitative RT-PCR analysis was performed with an ABI PRISM 7900 sequence detector (Applied Biosystems, Foster City, CA).

    Reverse Transcription:

    Article Title: Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling
    Article Snippet: .. After extraction with the use of RNeasy Mini Kits (QIAGEN, Valencia, CA), RNA samples were subjected to reverse transcription with SuperScript II RNase H reverse transcriptase (Invitrogen, Carlsbad, CA). .. Realtime quantitative RT-PCR analysis was performed with an ABI PRISM 7900 sequence detector (Applied Biosystems, Foster City, CA).

    Article Title: Gene expression profiles in BCL11B -siRNA treated malignant T cells
    Article Snippet: .. Total RNA was isolated using Trizol (Invitrogen), and cDNA was synthesized with a Superscript II RNaseH Reverse Transcriptase kit (Invitrogen). .. Total RNA (> 3 μg) was sent for global gene expression profile analysis using an Affymetrix HG U133 Plus 2.0 gene chip (Shanghai Biochip Co., Ltd., Shanghai, China).

    Article Title: The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process
    Article Snippet: .. RNAs used for sqRT-PCR were from independent experiments from those used in the cDNA library construction. cDNAs were synthesized by reverse transcription using the Superscript II RNAse H-reverse transcriptase (InvitrogenTM, Life Technologies). cDNAs were used for PCR in 30 μl reaction mixture containing specific primers, sense and antisense, as described in Table . ..

    Article Title: Gene expression patterns vary in clonal cell cultures from Rett syndrome females with eight different MECP2 mutations
    Article Snippet: .. Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript II Rnase H - Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers. ..

    Isolation:

    Article Title: Gene expression profiles in BCL11B -siRNA treated malignant T cells
    Article Snippet: .. Total RNA was isolated using Trizol (Invitrogen), and cDNA was synthesized with a Superscript II RNaseH Reverse Transcriptase kit (Invitrogen). .. Total RNA (> 3 μg) was sent for global gene expression profile analysis using an Affymetrix HG U133 Plus 2.0 gene chip (Shanghai Biochip Co., Ltd., Shanghai, China).

    Synthesized:

    Article Title: Gene expression profiles in BCL11B -siRNA treated malignant T cells
    Article Snippet: .. Total RNA was isolated using Trizol (Invitrogen), and cDNA was synthesized with a Superscript II RNaseH Reverse Transcriptase kit (Invitrogen). .. Total RNA (> 3 μg) was sent for global gene expression profile analysis using an Affymetrix HG U133 Plus 2.0 gene chip (Shanghai Biochip Co., Ltd., Shanghai, China).

    Article Title: The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process
    Article Snippet: .. RNAs used for sqRT-PCR were from independent experiments from those used in the cDNA library construction. cDNAs were synthesized by reverse transcription using the Superscript II RNAse H-reverse transcriptase (InvitrogenTM, Life Technologies). cDNAs were used for PCR in 30 μl reaction mixture containing specific primers, sense and antisense, as described in Table . ..

    cDNA Library Assay:

    Article Title: The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process
    Article Snippet: .. RNAs used for sqRT-PCR were from independent experiments from those used in the cDNA library construction. cDNAs were synthesized by reverse transcription using the Superscript II RNAse H-reverse transcriptase (InvitrogenTM, Life Technologies). cDNAs were used for PCR in 30 μl reaction mixture containing specific primers, sense and antisense, as described in Table . ..

    Polymerase Chain Reaction:

    Article Title: The transcriptome analysis of early morphogenesis in Paracoccidioides brasiliensis mycelium reveals novel and induced genes potentially associated to the dimorphic process
    Article Snippet: .. RNAs used for sqRT-PCR were from independent experiments from those used in the cDNA library construction. cDNAs were synthesized by reverse transcription using the Superscript II RNAse H-reverse transcriptase (InvitrogenTM, Life Technologies). cDNAs were used for PCR in 30 μl reaction mixture containing specific primers, sense and antisense, as described in Table . ..

    cDNA Synthesis:

    Article Title: Gene expression patterns vary in clonal cell cultures from Rett syndrome females with eight different MECP2 mutations
    Article Snippet: .. Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript II Rnase H - Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers. ..



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