superscript ii system (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher superscript ii system
Superscript Ii System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase+(ssii+rt)/Modification+Solution+II/pmc00312727-620-14-22
Average 94 stars, based on 84 article reviews

superscript ii system - by Bioz Stars, 2026-09

94/100 stars

Images

Mass Spectrometry:

Article Title: Diazirine-functionalized mannosides for photoaffinity labeling: trouble with FimH
Article Snippet: .. Nano-LC–MS was performed on the U3000 nano-LC-UV system (Dionex, Idstein, Germany) coupled online to a LTQ Orbitrap Velos mass spectrometer equipped with LTQ Tune Plus 2.7.0 and XCalibur 2.2 (all Thermo Fisher Scientific). ..

Construct:

Article Title: Complete mitochondrial genomes of three reef forming Acropora corals ( Acroporidae , Scleractinia ) from Chagos Archipelago, Indian Ocean
Article Snippet: The extraction followed a modified version of the manufacturer protocol for the DNeasy PowerSoil Pro Kit from Qiagen© (Protocols.io: dx.doi.org/10.17504/protocols.io.bww6pfhe ). .. Following quantification of double-stranded DNA with a Qubit fluorometer 2.0 (Invitrogen, Waltham, MA), three separate indexed libraries were constructed with the gDNA by ligation kit (Oxford Nanopore Technologies) and subsequently pooled, prior to sequencing on a MinION sequencer (ligation kit: SQK-LSK109; indexes: EXP-NBD104; flowcell: R9 FLO-MIN106D). ..

Ligation:

Sequencing:

Polymerase Chain Reaction:

Article Title: Association of L-Ficolin Levels and FCN2 Genotypes with Chronic Chagas Disease
Article Snippet: .. PCR amplifications were carried out in a Therm-2000 (Axygen, United States) or MJ96+ (Biocycler, China) research thermocyclers with 20 ng DNA, 0.2 mM dNTP (Invitrogen Life Technologies, United States), 0.2 μM of each primer (Invitrogen Life Technologies, United States), 1X Coral Load PCR buffer (Qiagen, The Netherlands), 2 mM of MgCl2 (Invitrogen Life Technologies, United States), 0.35–0.7 mM of Q solution (Invitrogen Life Technologies, United States), and 0.15 U Taq DNA polymerase (Invitrogen Life Technologies or Promega, United States) in a final volume of 15 μl. ..

Article Title: A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase
Article Snippet: Primers were synthesized and desalted by Integrated DNA technologies. .. All PCR reaction volumes were 50 μL with the following final reagent concentrations: 1X PCR Gold Buffer (ABI, Foster City, CA), 2.5 mM MgCl 2 solution (ABI, Foster City, CA), 0.05% BSA (NEB, Ipswich, MA), 0.2 mM dNTPs, 1 μM each primer, 2.5 U Amplitaq Gold DNA polymerase (ABI, Foster City, CA). ..

Article Title: Hypermethylation of the DPYD promoter region is not a major predictor of severe toxicity in 5-fluorouracil based chemotherapy
Article Snippet: A 209 bp fragment of the DPYD promoter region was amplified from BSM samples using a GeneAmp 9700 Thermal Cycler (Applied Biosystems) and the same primers as described in [ ]. .. The 25 μl PCR volume contained 1 μl of BSM DNA, 1 μl of each primer (10 μM), 1 μl of dNTP (10 mM each), 3 μl of MgCl 2 solution (25 mM), 2.5 μl of AmpliTaq Gold Buffer and 0.5 μl of AmpliTaq Gold DNA polymerase (Applied Biosystems). ..

other:

Article Title: Comparative genomics of Campylobacter jejuni from clinical campylobacteriosis stool specimens
Article Snippet: All plates throughout the protocol were incubated in a microaerophilic atmosphere using anaerobic jars with CampyGen 2.5 L sachet (Oxoid, Hampshire, United Kingdom) at 37 °C for 48 h. C. jejuni strain 81116 was used as a positive control throughout the protocol.

Review