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superscript ii® reverse transcriptase (m-mlv rt  (Thermo Fisher)


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    Thermo Fisher superscript ii® reverse transcriptase (m-mlv rt
    Superscript Ii® Reverse Transcriptase (M Mlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+m-mlv+reverse+transcriptase/pmc03699658-46-9-19
    Average 90 stars, based on 1 article reviews
    superscript ii® reverse transcriptase (m-mlv rt - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Oligoclonal CD4 + T Cells Promote Host Memory Immune Responses to Zwitterionic Polysaccharide of Streptococcus pneumoniae
    Article Snippet: Total RNA was extracted using an RNeasy plant mini kit (Qiagen). .. Synthesis of cDNA was carried out using 1 to 3 μg of total RNA with oligo(dT) and Moloney murine leukemia virus SuperScript II reverse transcriptase (Invitrogen) in a total volume of 50 μl of 1× Tris-acetate-EDTA buffer containing 10 mM dithiothreitol. ..

    Article Title: Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2.
    Article Snippet: RNA integrity and concentration were determined with the RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies). ..

    Article Title: Differences in hepatitis C virus core protein processing among genotypes 1 and 2.
    Article Snippet: a Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan b Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan c Fourth Department of Internal Medicine, Tokyo Medical University, Tokyo 160-0023, Japan d Department of Internal Medicine, Division of Gastroenterology and Hepatology, Jikei University School of Medicine (Daisan), Tokyo 201-8601, Japan e Division of Gastroenterology, John A. Burns School of Medicine, University of Hawaii, USA

    Article Title: Cortisol Exerts bi-phasic Regulation of Inflammation in Humans
    Article Snippet: RNA integrity and concentration were determined with RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. Expression of mRNA was quantified by Taqman PCR. cDNA (0.5 μl/well) was transferred into 96-well format plates, and Taqman Master Mix (Applied Biosystems) was added.

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    Article Title: Oligoclonal CD4 + T Cells Promote Host Memory Immune Responses to Zwitterionic Polysaccharide of Streptococcus pneumoniae
    Article Snippet: Total RNA was extracted using an RNeasy plant mini kit (Qiagen). .. Synthesis of cDNA was carried out using 1 to 3 g of total RNA with oligo(dT) and Moloney murine leukemia virus SuperScript II reverse transcriptase (Invitrogen) in a total volume of 50 l of 1 Tris-acetate-EDTA buffer containing 10 mM dithiothreitol. ..

    Article Title: Effects of inflammatory cytokines on the recurrence of liver cancer after an apparently curative operation.
    Article Snippet: METHODS: An experimental mouse model of liver cancer metastasis was designed using hepatoma 22(H22) inoculated into the subserous layer of spleen of 615 mice.. Partial hepatectomy (PH) or sham operation (SH) was performed at various periods of spleen inoculation and metastasic effects were recorded.. The expression of inflammatory cytokines as TNF-α, IL-1β, IL-6 and VCAM-1 were analyzed by reverse transcriptasepolymerase chain reaction (RT-PCR).

    Reverse Transcription:

    Article Title: Oligoclonal CD4 + T Cells Promote Host Memory Immune Responses to Zwitterionic Polysaccharide of Streptococcus pneumoniae
    Article Snippet: Total RNA was extracted using an RNeasy plant mini kit (Qiagen). .. Synthesis of cDNA was carried out using 1 to 3 μg of total RNA with oligo(dT) and Moloney murine leukemia virus SuperScript II reverse transcriptase (Invitrogen) in a total volume of 50 μl of 1× Tris-acetate-EDTA buffer containing 10 mM dithiothreitol. ..

    Article Title: Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2.
    Article Snippet: RNA integrity and concentration were determined with the RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies). ..

    Article Title: Differences in hepatitis C virus core protein processing among genotypes 1 and 2.
    Article Snippet: a Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan b Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan c Fourth Department of Internal Medicine, Tokyo Medical University, Tokyo 160-0023, Japan d Department of Internal Medicine, Division of Gastroenterology and Hepatology, Jikei University School of Medicine (Daisan), Tokyo 201-8601, Japan e Division of Gastroenterology, John A. Burns School of Medicine, University of Hawaii, USA

    Article Title: Cortisol Exerts bi-phasic Regulation of Inflammation in Humans
    Article Snippet: RNA integrity and concentration were determined with RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. Expression of mRNA was quantified by Taqman PCR. cDNA (0.5 μl/well) was transferred into 96-well format plates, and Taqman Master Mix (Applied Biosystems) was added.

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    Article Title: Oligoclonal CD4 + T Cells Promote Host Memory Immune Responses to Zwitterionic Polysaccharide of Streptococcus pneumoniae
    Article Snippet: Total RNA was extracted using an RNeasy plant mini kit (Qiagen). .. Synthesis of cDNA was carried out using 1 to 3 g of total RNA with oligo(dT) and Moloney murine leukemia virus SuperScript II reverse transcriptase (Invitrogen) in a total volume of 50 l of 1 Tris-acetate-EDTA buffer containing 10 mM dithiothreitol. ..

    Article Title: Effects of inflammatory cytokines on the recurrence of liver cancer after an apparently curative operation.
    Article Snippet: METHODS: An experimental mouse model of liver cancer metastasis was designed using hepatoma 22(H22) inoculated into the subserous layer of spleen of 615 mice.. Partial hepatectomy (PH) or sham operation (SH) was performed at various periods of spleen inoculation and metastasic effects were recorded.. The expression of inflammatory cytokines as TNF-α, IL-1β, IL-6 and VCAM-1 were analyzed by reverse transcriptasepolymerase chain reaction (RT-PCR).

    Synthesized:

    Article Title: Lipopolysaccharide-induced IL-1 beta production by human uterine macrophages up-regulates uterine epithelial cell expression of human beta-defensin 2.
    Article Snippet: RNA integrity and concentration were determined with the RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies). ..

    Article Title: Cortisol Exerts bi-phasic Regulation of Inflammation in Humans
    Article Snippet: RNA integrity and concentration were determined with RNA6000 Nano LabChip kit (Agilent). .. Using 500 ng of RNA as template, first-strand cDNA was synthesized using random hexamers and SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. Expression of mRNA was quantified by Taqman PCR. cDNA (0.5 μl/well) was transferred into 96-well format plates, and Taqman Master Mix (Applied Biosystems) was added.

    Polymerase Chain Reaction:

    Article Title: Differences in hepatitis C virus core protein processing among genotypes 1 and 2.
    Article Snippet: a Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan b Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan c Fourth Department of Internal Medicine, Tokyo Medical University, Tokyo 160-0023, Japan d Department of Internal Medicine, Division of Gastroenterology and Hepatology, Jikei University School of Medicine (Daisan), Tokyo 201-8601, Japan e Division of Gastroenterology, John A. Burns School of Medicine, University of Hawaii, USA

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    Amplification:

    Article Title: Differences in hepatitis C virus core protein processing among genotypes 1 and 2.
    Article Snippet: a Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan b Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan c Fourth Department of Internal Medicine, Tokyo Medical University, Tokyo 160-0023, Japan d Department of Internal Medicine, Division of Gastroenterology and Hepatology, Jikei University School of Medicine (Daisan), Tokyo 201-8601, Japan e Division of Gastroenterology, John A. Burns School of Medicine, University of Hawaii, USA

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    other:

    Article Title: UVB Irradiation Regulates ERK1/2- and p53-Dependent Thrombomodulin Expression in Human Keratinocytes
    Article Snippet: Complementary DNA (cDNA) was synthesized from total RNA using Moloney Murine Leukemia Virus SuperScript II® Reverse Transcriptase (M-MLV RT, Invitrogen, Carlsbad, CA), and PCR analysis was performed using cDNA as the template in a reaction mixture (25 μL) containing a specific primer pair for each cDNA.

    Incubation:

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    Random Hexamer:

    Article Title: Myogenic basic helix-loop-helix proteins regulate the expression of peroxisomal proliferator activated receptor-gamma coactivator-1alpha.
    Article Snippet: .. One microgram of total RNA was incubated for 5 min at 70 C with 100 U of the ribonuclease inhibitor HPRI (Amersham Biosciences) and random hexamer primers (New England Biolabs, Beverley, MA), then reverse transcribed for 90 min at 42 C using 200 U SuperScript II Moloney murine leukemia virus reverse transcriptase (Invitrogen Life Technologies, Inc.) in the presence of 200 m of each nucleotide triphosphate (deoxy-NTPs; Invitrogen Life Technologies, Inc.) in a total volume of 26 l. One height of the RT reaction (3 l) was used as a template for amplification by PCR using a thermal cycler (Stratagene). .. The amplification mixture contained cDNA, 25 pmol of each specific forward and reverse primer, 200 m deoxy-NTPs, 1.5 mm MgCl2, 5 l buffer, and 1.25 U Hot Star Taq DNA polymerase (QIAGEN, Hilden, Germany) in a total volume of 50 l. Primers were purchased from Sigma-Genosys Ltd. (Pampisford, UK).

    Article Title: Effects of inflammatory cytokines on the recurrence of liver cancer after an apparently curative operation.
    Article Snippet: METHODS: An experimental mouse model of liver cancer metastasis was designed using hepatoma 22(H22) inoculated into the subserous layer of spleen of 615 mice.. Partial hepatectomy (PH) or sham operation (SH) was performed at various periods of spleen inoculation and metastasic effects were recorded.. The expression of inflammatory cytokines as TNF-α, IL-1β, IL-6 and VCAM-1 were analyzed by reverse transcriptasepolymerase chain reaction (RT-PCR).



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