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Sangon Biotech superscript ii cdna synthesis kit
Superscript Ii Cdna Synthesis Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+kit/cdna+first+kit+mirna+strand+synthesis/pm42277600-52-21-26
Average 86 stars, based on 1 article reviews
superscript ii cdna synthesis kit - by Bioz Stars, 2026-11
86/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions. ..

cDNA Synthesis:

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions. ..

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: .. RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions.

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzol Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). .. qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

Article Title: Molecular Insights into Sex Differentiation of Rhinogobio nasutus via Integrated mRNA and miRNA Profiling
Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM First Strand cDNA Synthesis Kit (Takara, Kyoto, Japan) for mRNAs and the miRNA First Strand cDNA Synthesis Kit (Sangon Biotech, Shanghai, China) for miRNAs. .. Primers were designed with Primer Premier 6.0 (Tables 1 and 2). qRT PCR reactions were performed in triplicate on an ABI7500 Real-Time PCR system using TB Green® Premix Ex TaqTM II (Takara, Japan).

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Article Title: Cross-kingdom noncoding RNA regulation facilitates Nosema bombycis proliferation
Article Snippet: Total RNAs of various samples from mature spores, N. bombycis -infected BmE cells, and the N. bombycis -infected midgut were extracted using TRIzol reagent (Agbio, China) according to the manufacturer’s protocols. .. Next, the total RNAs were used for cDNA synthesis with the PrimeScriptTM RT reagent Kit (Takara, Japan) and miRNA first-strand cDNA synthesis (Tailing Reaction) (Sangon Biotech, China). .. Total RNAs of various samples from mature spores, N. bombycis -infected BmE cells, and the N. bombycis -infected midgut were extracted using TRIzol reagent (Agbio, China) according to the manufacturer’s protocols.

Synthesized:

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: Molecular Insights into Sex Differentiation of Rhinogobio nasutus via Integrated mRNA and miRNA Profiling
Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM First Strand cDNA Synthesis Kit (Takara, Kyoto, Japan) for mRNAs and the miRNA First Strand cDNA Synthesis Kit (Sangon Biotech, Shanghai, China) for miRNAs. .. Primers were designed with Primer Premier 6.0 (Tables 1 and 2). qRT PCR reactions were performed in triplicate on an ABI7500 Real-Time PCR system using TB Green® Premix Ex TaqTM II (Takara, Japan).

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Reverse Transcription:

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzol Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). .. qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

Purification:

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.



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