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superscript first strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa superscript first strand cdna synthesis kit
    Superscript First Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+kit/Mir-X+miRNA+First-Strand+Synthesis+Kit/pmc12412623-254-6-11
    Average 99 stars, based on 3837 article reviews
    superscript first strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems.
    Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Mir-X miRNA First Strand Synthesis kit (Takara) in 10 μL volume, and their performance as standards by qPCR was evaluated with 10-fold serial dilutions of the produced cDNA. qPCR was performed with the TB Green qRT-PCR kit (Takara) according to the manufacturer’s instructions using miRNA-specific F primers listed in Table S3 with a BioRad CFX96 Touch Real-Time PCR Detection System. ..

    Produced:

    Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems.
    Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Mir-X miRNA First Strand Synthesis kit (Takara) in 10 μL volume, and their performance as standards by qPCR was evaluated with 10-fold serial dilutions of the produced cDNA. qPCR was performed with the TB Green qRT-PCR kit (Takara) according to the manufacturer’s instructions using miRNA-specific F primers listed in Table S3 with a BioRad CFX96 Touch Real-Time PCR Detection System. ..

    Quantitative RT-PCR:

    Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems.
    Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Mir-X miRNA First Strand Synthesis kit (Takara) in 10 μL volume, and their performance as standards by qPCR was evaluated with 10-fold serial dilutions of the produced cDNA. qPCR was performed with the TB Green qRT-PCR kit (Takara) according to the manufacturer’s instructions using miRNA-specific F primers listed in Table S3 with a BioRad CFX96 Touch Real-Time PCR Detection System. ..

    Article Title: RNU2-1 gene copy number variations do not affect serum levels of miR-1246 as a biomarker for lung adenocarcinoma
    Article Snippet: .. MiR-X miRNA First-Strand Synthesis and TB Green qRT-PCR systems (Takara Bio Inc., cat. no. 638313) were used to quantify miR-1246. ..

    Generated:

    Article Title: The miR-221-5p/RAD18/RAD51 Axis Regulates DNA Damage Tolerance and Homologous Recombination to Drive Platinum Resistance in Ovarian Cancer
    Article Snippet: For mRNA quantification, cDNA was synthesized from of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen) with random hexamer primers. .. For miRNA quantification, cDNA was generated using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa Bio, Mountain View, CA), which utilizes a poly-A tailing strategy. .. Quantitative PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems) on a QuantStudio 12K Flex Real-Time PCR System.

    Isolation:

    Article Title: PCYT1B-Targeting miRNAs as Potential Biomarkers for Placental Diseases.
    Article Snippet: The solution was applied to a RNeasy MinElute spin column (Qiagen), washed according to the manufacturer’s protocol, and total RNA was finally eluted in 14 μL of RNase-free water. .. For miRNA analysis, isolated miRNAs were reverse-transcribed into cDNA using the Mir-X miRNA First-Strand Synthesis Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. ..

    Reverse Transcription:

    Article Title: PCYT1B-Targeting miRNAs as Potential Biomarkers for Placental Diseases.
    Article Snippet: The solution was applied to a RNeasy MinElute spin column (Qiagen), washed according to the manufacturer’s protocol, and total RNA was finally eluted in 14 μL of RNase-free water. .. For miRNA analysis, isolated miRNAs were reverse-transcribed into cDNA using the Mir-X miRNA First-Strand Synthesis Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions. ..

    Article Title: miR-206 Regulates Hypoxia-Induced Mitophagy and Phenotypic Remodeling in Yak Pulmonary Artery Smooth Muscle Cells
    Article Snippet: Hypoxia drives pulmonary artery smooth muscle cells (PASMCs) proliferation and phenotypic remodeling, processes central to vascular adaptation.. Yaks, native to high-altitude environments, provide a natural model for studying cellular responses to chronic hypoxia.. In this study, hypoxic exposure enhanced yak PASMCs proliferation and induced phenotypic transformation.

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094

    Article Title: Effect of the miR‑26a/b‑5p‑STAT3‑YKL‑40 regulatory axis on proliferation, migration, and invasion of endometrial cancer cells
    Article Snippet: Total miRNA or mRNA was extracted from HEC‐1A cells in healthy growth conditions using RNAiso Plus (cat. no. 9108, Takara Bio, Inc.). .. RNA was separated by adding chloroform, precipitated by adding isopropanol, washed with 75% ethanol, and finally dissolved in an appropriate volume of RNase‐free water. miRNA was reverse transcribed using a tailing method (Mir‐XTM miRNA First Strand Synthesis Kit; cat. no. 638315; Takara Bio, Inc.). ..

    Synthesized:

    Article Title: miR-206 Regulates Hypoxia-Induced Mitophagy and Phenotypic Remodeling in Yak Pulmonary Artery Smooth Muscle Cells
    Article Snippet: Hypoxia drives pulmonary artery smooth muscle cells (PASMCs) proliferation and phenotypic remodeling, processes central to vascular adaptation.. Yaks, native to high-altitude environments, provide a natural model for studying cellular responses to chronic hypoxia.. In this study, hypoxic exposure enhanced yak PASMCs proliferation and induced phenotypic transformation.

    cDNA Synthesis:

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094



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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control