superscript first strand cdna synthesis kit (TaKaRa)
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Superscript First Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/Mir-X+miRNA+First-Strand+Synthesis+Kit/pmc12412623-254-6-11
Average 99 stars, based on 3837 article reviews
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Real-time Polymerase Chain Reaction:Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems. Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Produced:Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems. Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Quantitative RT-PCR:Article Title: Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems. Article Snippet: .. 560 ng of each of 3 different synthetic miRNAs were used as input for standard cDNA production in 3 different reactions with the Article Title: RNU2-1 gene copy number variations do not affect serum levels of miR-1246 as a biomarker for lung adenocarcinoma Article Snippet: .. MiR-X miRNA First-Strand Synthesis and Generated:Article Title: The miR-221-5p/RAD18/RAD51 Axis Regulates DNA Damage Tolerance and Homologous Recombination to Drive Platinum Resistance in Ovarian Cancer Article Snippet: For mRNA quantification, cDNA was synthesized from of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen) with random hexamer primers. .. For miRNA quantification, cDNA was generated using the Mir-X miRNA Isolation:Article Title: PCYT1B-Targeting miRNAs as Potential Biomarkers for Placental Diseases. Article Snippet: The solution was applied to a RNeasy MinElute spin column (Qiagen), washed according to the manufacturer’s protocol, and total RNA was finally eluted in 14 μL of RNase-free water. .. For miRNA analysis, isolated miRNAs were reverse-transcribed into cDNA using the Mir-X miRNA Reverse Transcription:Article Title: PCYT1B-Targeting miRNAs as Potential Biomarkers for Placental Diseases. Article Snippet: The solution was applied to a RNeasy MinElute spin column (Qiagen), washed according to the manufacturer’s protocol, and total RNA was finally eluted in 14 μL of RNase-free water. .. For miRNA analysis, isolated miRNAs were reverse-transcribed into cDNA using the Mir-X miRNA Article Title: miR-206 Regulates Hypoxia-Induced Mitophagy and Phenotypic Remodeling in Yak Pulmonary Artery Smooth Muscle Cells Article Snippet: Hypoxia drives pulmonary artery smooth muscle cells (PASMCs) proliferation and phenotypic remodeling, processes central to vascular adaptation.. Yaks, native to high-altitude environments, provide a natural model for studying cellular responses to chronic hypoxia.. In this study, hypoxic exposure enhanced yak PASMCs proliferation and induced phenotypic transformation. Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome? Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples ( Article Title: Effect of the miR‑26a/b‑5p‑STAT3‑YKL‑40 regulatory axis on proliferation, migration, and invasion of endometrial cancer cells Article Snippet: Total miRNA or mRNA was extracted from HEC‐1A cells in healthy growth conditions using RNAiso Plus (cat. no. 9108, Takara Bio, Inc.). .. RNA was separated by adding chloroform, precipitated by adding isopropanol, washed with 75% ethanol, and finally dissolved in an appropriate volume of RNase‐free water. miRNA was reverse transcribed using a tailing method ( Synthesized:Article Title: miR-206 Regulates Hypoxia-Induced Mitophagy and Phenotypic Remodeling in Yak Pulmonary Artery Smooth Muscle Cells Article Snippet: Hypoxia drives pulmonary artery smooth muscle cells (PASMCs) proliferation and phenotypic remodeling, processes central to vascular adaptation.. Yaks, native to high-altitude environments, provide a natural model for studying cellular responses to chronic hypoxia.. In this study, hypoxic exposure enhanced yak PASMCs proliferation and induced phenotypic transformation. cDNA Synthesis:Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome? Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples ( |
