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superscript iv reverse transcriptase cdna synthesis kit  (TaKaRa)


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    TaKaRa superscript iv reverse transcriptase cdna synthesis kit
    Superscript Iv Reverse Transcriptase Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+kit/TB+Green+Premix+Ex+Taq/pmc12387645-241-21-35
    Average 99 stars, based on 11140 article reviews
    superscript iv reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    cDNA Synthesis:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Quantitative RT-PCR:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.
    Article Snippet: Next, 1 lg of RNA was aliquoted to a new tube and treated with DNase I (Thermo Fisher Scientific, Vilnius, Lithuania) at 37°C for 30 mins and used for cDNA synthesis with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Vilnius, Lithuania) according to the kit’s instructions. .. RT-qPCR was performed with TB green ex-Taq II (Takara Bio Europe, Saint-Germain-en-Laye, France) on the QuantStudio 5 Real-Time PCR machine (Applied Biosystems, Foster City, USA) in a reaction volume of 5 lL. .. Primers used for RT-qPCR (Table S1) were synthesized by Sigma Aldrich (Haverhill, UK).

    Article Title: Age-Dependent Flowering Transition in Cymbidium goeringii: Physiological Characterization and Small RNA Profiling.
    Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101-01, Vazyme, Nanjing, China) according to the manufacturer’s instructions. .. qRT-PCR was performed using TB Green Premix Ex Taq (RR420, Takara, Kyoto, Japan) in a 20 μL reaction volume. ..

    Article Title: Inhibition of miR-125b-1-3p alleviates acute kidney injury and mitochondrial membrane potential loss by targeting GSTK1.
    Article Snippet: As a type of regulated necrosis driven by iron-dependent lipid peroxidation, ferroptosis critically contributes to acute kidney injury (AKI).. Currently, whether miR-125b-1-3p is engaged in this pathological process remains unknown.. Here, miR-125b-1-3p was upregulated in kidney tissues from an AKI model (GSE125305), in serum from a small exploratory cohort (15 AKI patients vs 15 controls), and in hypoxia/reoxygenation (H/R)-treated NRK-52E cells and erastin-induced ferroptosis models (n = 3).

    Article Title: PDHA1 Hyperactivation-Mediated Glucose Metabolism Reprogramming Fuels Neutrophil NETosis and Promotes Acute Lung Injury.
    Article Snippet: Genomic DNA was removed and cDNA synthesized from 1 μg total RNA using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, RR047A). .. RT-qPCR was performed in triplicate with TB Green Premix Ex Taq II (Takara, RR420A) on a CFX96 Touch system (Bio-Rad Laboratories Ltd., UK). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris.
    Article Snippet: .. 24 Real-time PCR was performed using an ABI 7500 Fast Real-Time Sequence Detection System and 25 TB Green Premix Ex Taq (Takara Bio Inc.). ..

    Article Title: The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.
    Article Snippet: Next, 1 lg of RNA was aliquoted to a new tube and treated with DNase I (Thermo Fisher Scientific, Vilnius, Lithuania) at 37°C for 30 mins and used for cDNA synthesis with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Vilnius, Lithuania) according to the kit’s instructions. .. RT-qPCR was performed with TB green ex-Taq II (Takara Bio Europe, Saint-Germain-en-Laye, France) on the QuantStudio 5 Real-Time PCR machine (Applied Biosystems, Foster City, USA) in a reaction volume of 5 lL. .. Primers used for RT-qPCR (Table S1) were synthesized by Sigma Aldrich (Haverhill, UK).

    Article Title: NEK2 promotes trastuzumab resistance in gastric cancer through WTAP/YTHDF1-dependent m6A regulation of FN1.
    Article Snippet: 1 Department of Gastroenterology, Lishui Municipal Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, Zhejiang, China 2 Gastrointestinal Surgery, Lishui Municipal Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China Abstract Background Trastuzumab resistance remains a major challenge in the clinical management of gastric cancer (GC), and effective strategies to overcome resistance are still limited.. NEK2 has been implicated in GC progression and therapeutic resistance in several tumor types; however, its role in trastuzumab resistance in GC and the underlying mechanisms remain unclear.. Methods and Results Trastuzumab-resistant NCI-N87/TR cells were established from parental NCI-N87 cells by stepwise exposure to increasing concentrations of trastuzumab.

    Sequencing:

    Article Title: A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris.
    Article Snippet: .. 24 Real-time PCR was performed using an ABI 7500 Fast Real-Time Sequence Detection System and 25 TB Green Premix Ex Taq (Takara Bio Inc.). ..

    other:

    Article Title: STING engages PKR to drive lysosomal cell death in lung ischemia-reperfusion injury.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0521; RRID: AB_2923334 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC Beyotime Cat# A0562; RRID: AB_2923335 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0516; RRID: AB_2893015 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Beyotime Cat# A0473; RRID: AB_2891322 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0502; RRID: AB_3065047 InVivoSIM anti-human IFNAR1 (Anifrolumab Biosimilar) BioXcell Cat# SIM0022; RRID: AB_3696361 Chemicals, Peptides, and Recombinant Proteins Phosphatase inhibitor cocktail Sigma-Aldrich Cat# P5726 Methyl Cellulose Sigma-Aldrich Cat# M0512 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 Lipofectamine 2000 Reagent ThermoFisher Scientific Cat# L11668019 Lipofectamine 3000 Reagent ThermoFisher Scientific Cat# L3000001 Lipo8000 Transfection Reagent Beyotime Cat# C0533 2’3′-cGAMP Invivogen Cat# tlrl-nacga23-02 DNase I Roche Cat# 9003-98-9 Collagenase Type I ThermoFisher Scientific Cat# 17100017 Dispase Roche Cat# 40104ES80 Palbociclib MedChemExpress Cat# HY-50767 Amlexanox MedChemExpress Cat# HY-B0713 BAY 11-7082 MedChemExpress Cat# HY-13453 2’,3’-dideoxycytidine MedChemExpress Cat# HY-17392 GSK2606414 MedChemExpress Cat# HY-18072 Brefeldin A MedChemExpress Cat# HY-16592 5Z-7-Oxozeaenol MedChemExpress Cat#HY-12686 TUDCA MedChemExpress Cat#HY-19696 LLOMe MedChemExpress Cat#HY-129905 Bafilomycin A1 MedChemExpress Cat#HY-100558 E64d MedChemExpress Cat#HY-100229 CA-074Me MedChemExpress Cat#HY-100350 Z-VAD-FMK MedChemExpress Cat#HY-16658B Ferrostatin-1 MedChemExpress Cat#HY-100579 Recombinant Human IFN-β Peprotech Cat#300-02BC Triton X-100 Sigma-Aldrich Cat#T8787 Digitonin Sigma-Aldrich Cat# 300410 Cls IV Sigma-Aldrich Cat# C4BIOC Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Critical Commercial Assay TRIzol Reagent ThermoFisher Scientific Cat# 15596-018 TB Green Premix Ex Taq Takara Cat# RR420B Pierce Co-immunoprecipitation kit ThermoFisher Scientific Cat# 26149 (Continued on next page) ll Article Molecular Cell 86, 1–19.e1–e11, November 5, 2026 e2



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    Fisher Scientific superscript iii cellsdirect cdna synthesis kit
    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control