superscript iv reverse transcriptase cdna synthesis kit (TaKaRa)
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Superscript Iv Reverse Transcriptase Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 11140 article reviews
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Reverse Transcription:Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using cDNA Synthesis:Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using Quantitative RT-PCR:Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using Article Title: The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation. Article Snippet: Next, 1 lg of RNA was aliquoted to a new tube and treated with DNase I (Thermo Fisher Scientific, Vilnius, Lithuania) at 37°C for 30 mins and used for cDNA synthesis with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Vilnius, Lithuania) according to the kit’s instructions. .. RT-qPCR was performed with Article Title: Age-Dependent Flowering Transition in Cymbidium goeringii: Physiological Characterization and Small RNA Profiling. Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101-01, Vazyme, Nanjing, China) according to the manufacturer’s instructions. .. qRT-PCR was performed using Article Title: Inhibition of miR-125b-1-3p alleviates acute kidney injury and mitochondrial membrane potential loss by targeting GSTK1. Article Snippet: As a type of regulated necrosis driven by iron-dependent lipid peroxidation, ferroptosis critically contributes to acute kidney injury (AKI).. Currently, whether miR-125b-1-3p is engaged in this pathological process remains unknown.. Here, miR-125b-1-3p was upregulated in kidney tissues from an AKI model (GSE125305), in serum from a small exploratory cohort (15 AKI patients vs 15 controls), and in hypoxia/reoxygenation (H/R)-treated NRK-52E cells and erastin-induced ferroptosis models (n = 3). Article Title: PDHA1 Hyperactivation-Mediated Glucose Metabolism Reprogramming Fuels Neutrophil NETosis and Promotes Acute Lung Injury. Article Snippet: Genomic DNA was removed and cDNA synthesized from 1 μg total RNA using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, RR047A). .. RT-qPCR was performed in triplicate with Real-time Polymerase Chain Reaction:Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using Article Title: A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris. Article Snippet: .. 24 Real-time PCR was performed using an ABI 7500 Fast Real-Time Sequence Detection System and 25 Article Title: The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation. Article Snippet: Next, 1 lg of RNA was aliquoted to a new tube and treated with DNase I (Thermo Fisher Scientific, Vilnius, Lithuania) at 37°C for 30 mins and used for cDNA synthesis with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Vilnius, Lithuania) according to the kit’s instructions. .. RT-qPCR was performed with Article Title: NEK2 promotes trastuzumab resistance in gastric cancer through WTAP/YTHDF1-dependent m6A regulation of FN1. Article Snippet: 1 Department of Gastroenterology, Lishui Municipal Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, Zhejiang, China 2 Gastrointestinal Surgery, Lishui Municipal Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China Abstract Background Trastuzumab resistance remains a major challenge in the clinical management of gastric cancer (GC), and effective strategies to overcome resistance are still limited.. NEK2 has been implicated in GC progression and therapeutic resistance in several tumor types; however, its role in trastuzumab resistance in GC and the underlying mechanisms remain unclear.. Methods and Results Trastuzumab-resistant NCI-N87/TR cells were established from parental NCI-N87 cells by stepwise exposure to increasing concentrations of trastuzumab. Sequencing:Article Title: A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris. Article Snippet: .. 24 Real-time PCR was performed using an ABI 7500 Fast Real-Time Sequence Detection System and 25 other:Article Title: STING engages PKR to drive lysosomal cell death in lung ischemia-reperfusion injury. 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