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Fisher Scientific superscript iii cellsdirect cdna synthesis kit
RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Superscript Iii Cellsdirect Cdna Synthesis Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/capacity+cdna+high+kit+reverse+transcription/pmc12327944-57-5-12
Average 86 stars, based on 1 article reviews
superscript iii cellsdirect cdna synthesis kit - by Bioz Stars, 2026-09
86/100 stars

Images

1) Product Images from "Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish"

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

Journal: eLife

doi: 10.7554/eLife.97400

RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
Figure Legend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control

Related Articles

cDNA Synthesis:

Article Title: Uncovering the mechanisms of clinically relevant altered antibiotic responses of Staphylococcus aureus under wound infection-mimetic conditions.
Article Snippet: Aliquots (6 mL) of the cultures were collected, centrifuged at 3,320 × g for 10 min. RNA was extracted using Monarch Total RNA miniprep (New England Biolabs), followed by treatment with Invitrogen Turbo DNA-free DNase (Fisher Scientific) following the manufacturer’s protocol. .. RNA purity was assessed by measuring the ratio of absorbance at 260 nm to 280 nm (values obtained ∼2.0). cDNA synthesis and RT-PCR RNA was converted to cDNA with SuperscriptTM III First-Strand Synthesis System (Fisher Scientific) following the manufacturer’s protocol. .. RT-PCR was performed using the QuantStudioTM 5 Real-Time PCR (Thermo Fisher Scientific) with FastStart SYBR Green (Roche).

Article Title: Matrix-bound Tenascin-C directs neuronal differentiation through stiffness-tuned MeHA hydrogels mimicking the spinal cord microenvironment.
Article Snippet: Spinal cord injury (SCI) leads to a complex remodeling of the extracellular matrix (ECM), where Tenascin-C (TNC) is strongly upregulated during the early phases of the injury cascade.. While TNC is known to influence neural cell behavior, its functional role and mode of presentation in guiding neuronal differentiation remains unclear.. In this study, we developed a stiffness-controlled methacrylated hyaluronic acid (MeHA) hydrogel platform that mimics the mechanical properties of the spinal cord and enables defined matrix immobilization of TNC.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Uncovering the mechanisms of clinically relevant altered antibiotic responses of Staphylococcus aureus under wound infection-mimetic conditions.
Article Snippet: Aliquots (6 mL) of the cultures were collected, centrifuged at 3,320 × g for 10 min. RNA was extracted using Monarch Total RNA miniprep (New England Biolabs), followed by treatment with Invitrogen Turbo DNA-free DNase (Fisher Scientific) following the manufacturer’s protocol. .. RNA purity was assessed by measuring the ratio of absorbance at 260 nm to 280 nm (values obtained ∼2.0). cDNA synthesis and RT-PCR RNA was converted to cDNA with SuperscriptTM III First-Strand Synthesis System (Fisher Scientific) following the manufacturer’s protocol. .. RT-PCR was performed using the QuantStudioTM 5 Real-Time PCR (Thermo Fisher Scientific) with FastStart SYBR Green (Roche).

Reverse Transcription:

Article Title: Matrix-bound Tenascin-C directs neuronal differentiation through stiffness-tuned MeHA hydrogels mimicking the spinal cord microenvironment.
Article Snippet: Spinal cord injury (SCI) leads to a complex remodeling of the extracellular matrix (ECM), where Tenascin-C (TNC) is strongly upregulated during the early phases of the injury cascade.. While TNC is known to influence neural cell behavior, its functional role and mode of presentation in guiding neuronal differentiation remains unclear.. In this study, we developed a stiffness-controlled methacrylated hyaluronic acid (MeHA) hydrogel platform that mimics the mechanical properties of the spinal cord and enables defined matrix immobilization of TNC.

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

Viscosity:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

Modification:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

dsDNA Assay:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.



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RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome <t>cDNA</t> was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.
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Image Search Results


RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Journal: eLife

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

doi: 10.7554/eLife.97400

Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control