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Sangon Biotech superscript ii cdna synthesis kit
Superscript Ii Cdna Synthesis Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+cdna+kit/cdna+first+kit+mirna+strand+synthesis/pm42277600-52-21-26
Average 86 stars, based on 1 article reviews
superscript ii cdna synthesis kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions. ..

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzolTM Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file. mRNA or lncRNA expression was calculated, referring to that of the gene Gapdh , while piRNA expression was calculated, referring to that of the gene U6 . .. Each experiment was repeated three times. mRNA or lncRNA PCR amplification was performed using a PCR kit (Phanta®Max Super-Fidelity DNA Polymerase, Vazyme, Cat# P505-d1) on a PCR machine (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

cDNA Synthesis:

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions. ..

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: FTO-Catalysed Demethylation of LUR1 mRNA Suppresses Macrophage Lipid Accumulation and Aortic Atherosclerosis.
Article Snippet: .. RNA was extracted using TRIzol Reagent (Sangon Biotech, B511311, China). cDNA was prepared by amplifying 500 ng of RNA with a SuperScript- II cDNA Synthesis Kit (Sangon Biotech, B639277, China). .. Quantitative PCR was performed using a RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech, B300538, China) with 1 mg of RNA, and qRT–PCR was performed using an UltraSYBR One Step RT–qPCR Kit (Sangon Biotech, B300540, China) following the manufacturer's instructions.

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzolTM Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file. mRNA or lncRNA expression was calculated, referring to that of the gene Gapdh , while piRNA expression was calculated, referring to that of the gene U6 . .. Each experiment was repeated three times. mRNA or lncRNA PCR amplification was performed using a PCR kit (Phanta®Max Super-Fidelity DNA Polymerase, Vazyme, Cat# P505-d1) on a PCR machine (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

Article Title: Molecular Insights into Sex Differentiation of Rhinogobio nasutus via Integrated mRNA and miRNA Profiling
Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM First Strand cDNA Synthesis Kit (Takara, Kyoto, Japan) for mRNAs and the miRNA First Strand cDNA Synthesis Kit (Sangon Biotech, Shanghai, China) for miRNAs. .. Primers were designed with Primer Premier 6.0 (Tables 1 and 2). qRT PCR reactions were performed in triplicate on an ABI7500 Real-Time PCR system using TB Green® Premix Ex TaqTM II (Takara, Japan).

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Article Title: Cross-kingdom noncoding RNA regulation facilitates Nosema bombycis proliferation
Article Snippet: Total RNAs of various samples from mature spores, N. bombycis -infected BmE cells, and the N. bombycis -infected midgut were extracted using TRIzol reagent (Agbio, China) according to the manufacturer’s protocols. .. Next, the total RNAs were used for cDNA synthesis with the PrimeScriptTM RT reagent Kit (Takara, Japan) and miRNA first-strand cDNA synthesis (Tailing Reaction) (Sangon Biotech, China). ..

Synthesized:

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: Molecular Insights into Sex Differentiation of Rhinogobio nasutus via Integrated mRNA and miRNA Profiling
Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM First Strand cDNA Synthesis Kit (Takara, Kyoto, Japan) for mRNAs and the miRNA First Strand cDNA Synthesis Kit (Sangon Biotech, Shanghai, China) for miRNAs. .. Primers were designed with Primer Premier 6.0 (Tables 1 and 2). qRT PCR reactions were performed in triplicate on an ABI7500 Real-Time PCR system using TB Green® Premix Ex TaqTM II (Takara, Japan).

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.

Reverse Transcription:

Article Title: RNA structure programs endogenous ADAR for precise and efficient editing.
Article Snippet: Article RNA structure programs endogenous ADAR for precise and efficient editing

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzolTM Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file. mRNA or lncRNA expression was calculated, referring to that of the gene Gapdh , while piRNA expression was calculated, referring to that of the gene U6 . .. Each experiment was repeated three times. mRNA or lncRNA PCR amplification was performed using a PCR kit (Phanta®Max Super-Fidelity DNA Polymerase, Vazyme, Cat# P505-d1) on a PCR machine (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

SYBR Green Assay:

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzolTM Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file. mRNA or lncRNA expression was calculated, referring to that of the gene Gapdh , while piRNA expression was calculated, referring to that of the gene U6 . .. Each experiment was repeated three times. mRNA or lncRNA PCR amplification was performed using a PCR kit (Phanta®Max Super-Fidelity DNA Polymerase, Vazyme, Cat# P505-d1) on a PCR machine (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

Expressing:

Article Title: The pachytene-specific lncRNA 1700008K24Rik is essential for mouse spermatogenesis and functions as a conserved piRNA precursor
Article Snippet: RNA extraction was performed using TRIzol reagent (TRIzolTM Reagent, Thermo Scientific, Cat#15596026). .. Reverse transcription of mRNAs or lncRNAs was performed using a reverse transcription kit (HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper), Vazyme, Cat# R212-01), while reverse transcription of piRNAs was carried out using a piRNA reverse transcription kit (miRNA First Strand cDNA Synthesis Kit (Tailing Reaction), Sangon Biotech, Cat# B532451). qPCR was performed via qPCR reagent (AceQ qPCR SYBR Green Master Mix, Vazyme, Cat# Q111-02) on a qPCR detection system (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file. mRNA or lncRNA expression was calculated, referring to that of the gene Gapdh , while piRNA expression was calculated, referring to that of the gene U6 . .. Each experiment was repeated three times. mRNA or lncRNA PCR amplification was performed using a PCR kit (Phanta®Max Super-Fidelity DNA Polymerase, Vazyme, Cat# P505-d1) on a PCR machine (Bio-Rad) using the specific primers listed in in the “Multimedia component 3” file.

Purification:

Article Title: Zebrafish (Danio rerio)-based assessment of Rhododendron dauricum developmental and cardiotoxicity: Calcium overload, oxidative stress, and apoptosis mediated by rhododendrol.
Article Snippet: Ethnopharmacological relevance: Rhododendron dauricum L. is used in traditional medicine for respiratory disorders, but its developmental and cardiac safety remains insufficiently defined.. Aim of the study: To characterize developmental/cardiac phenotypes induced by Rhododendron dauricum hotwater extract (RDWE), identify toxicity-enriched fractions and candidate constituents, and assess whether selective fraction removal reduces toxicity-related effects.. Materials and methods: Zebrafish embryos/larvae were exposed to RDWE, four membrane fractions, and candidate monomers.



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Image Search Results


RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Journal: eLife

Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

doi: 10.7554/eLife.97400

Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control