superscript cdna synthesis kit (TaKaRa)
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Superscript Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quantitative RT-PCR:Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis. Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a Fluorescence:Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis. Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Real-time Polymerase Chain Reaction:Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis. Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a Synthesized:Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis. Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs. Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA Synthesis:Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis. Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD Article Snippet: .. Reverse transcription was carried out using a commercial Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs. Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer Article Title: Dietary supplementation with Bacillus velezensis enhances the intestinal health of broiler chickens challenged with necrotic enteritis via reshaping the structure and function of the intestinal flora Article Snippet: .. Then, the gDNA Eraser (Takara, Dalian, China) was used to remove contaminating genomic DNA, and Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome? Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one Reverse Transcription:Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis Article Snippet: .. Reverse transcription was performed using a Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD Article Snippet: .. Reverse transcription was carried out using a commercial Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome? Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one Sequencing:Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer Polymerase Chain Reaction:Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs. Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer Article Title: Antigen-binding molecule for promoting disappearance of antigen via FcγRIIB Article Snippet: Alternatively, kits for extracting total mRNA directly from cells, such as the QuickPrep mRNA Purification Kit (GE Healthcare Bioscience), are also commercially available. mRNAs can be prepared from hybridomas using such kits. cDNAs encoding the antibody V region can be synthesized from the prepared mRNAs using a reverse transcriptase. cDNAs can be synthesized using the AMV Reverse Transcriptase First-strand cDNA Synthesis Kit (Seikagaku Co.) or such. .. Furthermore, the Amplification:Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs. Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer Article Title: Antigen-binding molecule for promoting disappearance of antigen via FcγRIIB Article Snippet: Alternatively, kits for extracting total mRNA directly from cells, such as the QuickPrep mRNA Purification Kit (GE Healthcare Bioscience), are also commercially available. mRNAs can be prepared from hybridomas using such kits. cDNAs encoding the antibody V region can be synthesized from the prepared mRNAs using a reverse transcriptase. cDNAs can be synthesized using the AMV Reverse Transcriptase First-strand cDNA Synthesis Kit (Seikagaku Co.) or such. .. Furthermore, the |
