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superscript cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa superscript cdna synthesis kit
    Superscript Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+cdna+kit/SMARTer+PCR+cDNA+Synthesis+Kit/pm40921884-94-9-13
    Average 96 stars, based on 6067 article reviews
    superscript cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis.
    Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Fluorescence:

    Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis.
    Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Real-time Polymerase Chain Reaction:

    Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis.
    Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Synthesized:

    Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis.
    Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata
    Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Takara Biotechnology, Dalian, China). ..

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    cDNA Synthesis:

    Article Title: Targeting DYRK1A with harmine abrogates the FOXO1-NF-κB axis to alleviate cellular senescence and renal fibrosis.
    Article Snippet: Journal Pre-proof Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue, Limin Liu PII: S0047-6374(26)00036-9 DOI: https://doi.org/10.1016/j.mad.2026.112184 Reference: MAD112184 To appear in: Mechanisms of Ageing and Development Received date: 21 September 2025 Revised date: 7 April 2026 Accepted date: 16 April 2026 Please cite this article as: Pengfei Qiao, Yixuan Zhu, Junming Zhang, Yukun Gan, Yan Wang, Yushan Li, Zhichao Li, Haiyan Xue and Limin Liu, Targeting DYRK1A with Harmine Abrogates the FOXO1-NF-κB Axis to Alleviate Cellular Senescence and Renal Fibrosis, Mechanisms of Ageing and Development, (2026) doi:https://doi.org/10.1016/j.mad.2026.112184 This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata
    Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Takara Biotechnology, Dalian, China). ..

    Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD
    Article Snippet: .. Reverse transcription was carried out using a commercial cDNA synthesis kit (Takara, Shiga, Japan). ..

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Article Title: Dietary supplementation with Bacillus velezensis enhances the intestinal health of broiler chickens challenged with necrotic enteritis via reshaping the structure and function of the intestinal flora
    Article Snippet: .. Then, the gDNA Eraser (Takara, Dalian, China) was used to remove contaminating genomic DNA, and cDNA synthesis kit (Takara, Dalian, China) was used to reverse it into stabilized cDNA. .. The stable cDNA, the primers for the target gene, and the fluorescent dye were mixed and tested on the fluorescence quantitative PCR instrument (an applied biosystems 7500 fast Real-time PCR system, Foster City, CA).

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094

    Reverse Transcription:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD
    Article Snippet: .. Reverse transcription was carried out using a commercial cDNA synthesis kit (Takara, Shiga, Japan). ..

    Article Title: Exosomal CNP and CNP-Related microRNAs: An Open Window into Brugada Syndrome?
    Article Snippet: RNA integrity, purity, and concentration were assessed by measuring absorbance at 260 and 280 nm (NanoDrop Thermofisher, Waltham, MA, USA) and applying the Beer–Lambert law with expected values between 1.8 and 2.1, for protein contamination. .. Exosomal miRNA reverse transcription was performed using an all-in-one cDNA Synthesis Kit (Takara Bio Inc., Kusatsu, Shiga, Japan) that provides a comprehensive solution for the efficient synthesis of first-strand miRNA cDNA from RNA samples (Mir-X miRNA First-Strand Synthesis Kit, Takara, Takara Bio Inc., Kusatsu, Shiga, Japan). .. The reverse transcription was performed in a thermocycler (My Cycler, (Bio-Rad Laboratories, Hercules, CA, USA) with an incubation step for 1 h at 37 ◦C; then the reaction was stopped by heating the mixture for 5 min at 85 ◦C, to inactivate the enzymes, prior to a final hold at 4 ◦C. https://doi.org/10.3390/biomedicines14051094

    Sequencing:

    Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata
    Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Takara Biotechnology, Dalian, China). ..

    Polymerase Chain Reaction:

    Article Title: Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata
    Article Snippet: Illumina RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit following the manufacturer’s protocols and sequenced on an Illumina NovaSeq 6000 platform. .. For single-molecule real-time isoform sequencing (Iso-Seq) sequencing, full-length cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Takara Biotechnology, Dalian, China). ..

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Article Title: Antigen-binding molecule for promoting disappearance of antigen via FcγRIIB
    Article Snippet: Alternatively, kits for extracting total mRNA directly from cells, such as the QuickPrep mRNA Purification Kit (GE Healthcare Bioscience), are also commercially available. mRNAs can be prepared from hybridomas using such kits. cDNAs encoding the antibody V region can be synthesized from the prepared mRNAs using a reverse transcriptase. cDNAs can be synthesized using the AMV Reverse Transcriptase First-strand cDNA Synthesis Kit (Seikagaku Co.) or such. .. Furthermore, the SMART RACE cDNA Amplification Kit (Clontech) and the PCR-based 5′-RACE method (Proc. ..

    Amplification:

    Article Title: Alternative splicing and polyadenylation facilitate transcriptome diversity underlying high-altitude adaptation in pigs.
    Article Snippet: .. Poly(A)+ mRNA cDNA was synthesized using the SMARTer cDNA synthesis kit (Clontech, USA), and full-length cDNA was then amplified through PCR employing PrimeSTAR GXL DNA polymerase (Takara, China). ..

    Article Title: Antigen-binding molecule for promoting disappearance of antigen via FcγRIIB
    Article Snippet: Alternatively, kits for extracting total mRNA directly from cells, such as the QuickPrep mRNA Purification Kit (GE Healthcare Bioscience), are also commercially available. mRNAs can be prepared from hybridomas using such kits. cDNAs encoding the antibody V region can be synthesized from the prepared mRNAs using a reverse transcriptase. cDNAs can be synthesized using the AMV Reverse Transcriptase First-strand cDNA Synthesis Kit (Seikagaku Co.) or such. .. Furthermore, the SMART RACE cDNA Amplification Kit (Clontech) and the PCR-based 5′-RACE method (Proc. ..



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    Image Search Results


    RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Journal: eLife

    Article Title: Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

    doi: 10.7554/eLife.97400

    Figure Lengend Snippet: RT-PCR analysis revealed the endogenous expression of krt4, krtt1c19e, notch1a, notch 2, notch 3, and dlc in keratinocytes. These cells were FACS-sorted for EGFP+ cells from Tg(krt4:lyn-EGFP ) and for tdTomato+ cells from Tg(krtt1c19e:tdTomato ). Whole genome cDNA was used as a positive control, and reactions without a template served as negative controls. Figure 4—figure supplement 1—source data 1. Original gel images for RT-PCR analysis displayed in , with labels. Figure 4—figure supplement 1—source data 2. Original files for RT-PCR analysis displayed in , without labels.

    Article Snippet: Commercial assay or kit , SuperScript III CellsDirect cDNA Synthesis Kit , Fisher Scientific , 18-080-200 , .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control