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cdna preparation kit (superscript lambda system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cdna preparation kit (superscript lambda system
    Cdna Preparation Kit (Superscript Lambda System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript%E2%84%A2+lambda+system+kit/us07993643-464-27-33
    Average 90 stars, based on 1 article reviews
    cdna preparation kit (superscript lambda system - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice
    Article Snippet: Freshly isolated PHHs with over 90% viability were obtained from BioIVT (Baltimore, MD) and seeded at 0.75 × 10 6 cells/well in a 12-well biocoat plate in INVITROGRO CP Medium (BioIVT). .. On the second day after cells attached, PHHs were overlaid with Matrigel (0.25 mg/mL) for sandwich culture in complete Williams’ E medium before treatment with 0.1% DMSO, CITCO (1 μM), RIF (10 μM), FDN (10 μM), E2 (3 μM) and EE2 (3 μM) or FDN + E2 and FDN + EE2, respectively for another 24 h. Cells were harvested using TRIzol reagent (Thermo Fisher, Rockford, IL) and total RNA was isolated and reverse transcribed to cDNA using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) following the manufacturer’s instructions. .. RT-PCR assays were performed on an ABI StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Mastermix (Qiagen).

    Article Title: An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice.
    Article Snippet: Primary human hepatocyte (PHH) assay Freshly isolated PHHs with over 90% viability were obtained from BioIVT (Baltimore, MD) and seeded at 0.75 × 106 cells/well in a 12-well biocoat plate in INVITROGRO CPMedium (BioIVT). .. On the second day after cells attached, PHHswereoverlaidwithMatrigel (0.25mg/mL) for sandwich culture in complete Williams’ E medium before treatment with 0.1%DMSO, CITCO (1 μM), RIF (10 μM), FDN (10 μM), E2 (3 μM) and EE2 (3 μM) or FDN+ E2 and FDN+ EE2, respectively for another 24 h. Cells were harvested using TRIzol reagent (Thermo Fisher, Rockford, IL) and total RNAwas isolated and reverse transcribed to cDNA using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) following the manufacturer’s instructions. .. RT-PCR assays were performed on an ABI StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Mastermix (Qiagen).

    Article Title: Geniposide Protects Against Myocardial Infarction Injury via the Restoration in Gut Microbiota and Gut–Brain Axis
    Article Snippet: The supernatant was collected to assess malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) activity according to the instructions provided by the kits (NanJing JianCheng Bioengineering Institute) [ ]. .. Total RNA was isolated from myocardium using Trizol reagent (Invitrogen). cDNA was transcribed using a cDNA Archive Kit (Applied Biosystems). .. Quantitative real‐time PCR (qRT‐PCR) was performed using an ABI Prism 7900 Sequence Detection System (Applied Biosystems).

    Reverse Transcription:

    Article Title: An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice
    Article Snippet: Freshly isolated PHHs with over 90% viability were obtained from BioIVT (Baltimore, MD) and seeded at 0.75 × 10 6 cells/well in a 12-well biocoat plate in INVITROGRO CP Medium (BioIVT). .. On the second day after cells attached, PHHs were overlaid with Matrigel (0.25 mg/mL) for sandwich culture in complete Williams’ E medium before treatment with 0.1% DMSO, CITCO (1 μM), RIF (10 μM), FDN (10 μM), E2 (3 μM) and EE2 (3 μM) or FDN + E2 and FDN + EE2, respectively for another 24 h. Cells were harvested using TRIzol reagent (Thermo Fisher, Rockford, IL) and total RNA was isolated and reverse transcribed to cDNA using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) following the manufacturer’s instructions. .. RT-PCR assays were performed on an ABI StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Mastermix (Qiagen).

    Article Title: An abundant ginger compound furanodienone alleviates gut inflammation via the xenobiotic nuclear receptor PXR in mice.
    Article Snippet: Primary human hepatocyte (PHH) assay Freshly isolated PHHs with over 90% viability were obtained from BioIVT (Baltimore, MD) and seeded at 0.75 × 106 cells/well in a 12-well biocoat plate in INVITROGRO CPMedium (BioIVT). .. On the second day after cells attached, PHHswereoverlaidwithMatrigel (0.25mg/mL) for sandwich culture in complete Williams’ E medium before treatment with 0.1%DMSO, CITCO (1 μM), RIF (10 μM), FDN (10 μM), E2 (3 μM) and EE2 (3 μM) or FDN+ E2 and FDN+ EE2, respectively for another 24 h. Cells were harvested using TRIzol reagent (Thermo Fisher, Rockford, IL) and total RNAwas isolated and reverse transcribed to cDNA using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA) following the manufacturer’s instructions. .. RT-PCR assays were performed on an ABI StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Mastermix (Qiagen).

    other:

    Article Title: Estrogen-related receptor gamma is a regulator of mitochondrial, autophagy, and immediate-early gene programs in spiny projection neurons: Relevance for transcriptional changes in Huntington disease.
    Article Snippet: Mitochondrial dysfunction, transcriptional dysregulation, and protein aggregation are hallmarks of multiple neurodegenerative disorders, including Huntington’s disease (HD).. Strategies are needed to counteract these processes to restore neuronal health and function in HD.. Recent evidence indicates that the transcription factor estrogen-related receptor gamma (ERRγ/Esrrg) is required for normal expression of mitochondrial, synaptic, and autophagy genes in neurons.

    Article Title: Melatonin improves the lead tolerance in Plantago ovata by modulating ROS homeostasis, phytohormone status and expression of stress-responsive genes.
    Article Snippet: Key message Melatonin increases Pb tolerance in P. ovata seedlings via the regulation of growth and stress-related phytohormones, ROS scavenging and genes responsible for melatonin synthesis, metal chelation, and stress defense.. Abstract Lead (Pb) is a highly toxic heavy metal that accumulates in plants through soil and air contamination and impairs its plant growth and development.. Because of its pharmaceutical importance, improvements in Plantago ovata yield against abiotic stresses are necessary.

    Article Title: An immunocompetent mouse model of liposarcoma
    Article Snippet: RNA was quantified using the NanoDrop 2000 and 200 ng was used for reverse transcription with the High-Capacity cDNA RT kit (Applied Biosystems #4368814).

    Article Title: Epigenetic dysregulation of metabolic programs mediates liposarcoma cell plasticity
    Article Snippet: For cDNA synthesis, 0.5 - 1 μg of total RNA was reverse transcribed using the High-capacity cDNA RT kit (Applied Biosystems) according to the manufacturer’s instruction and performed in a PTC Tempo Thermal Cycler (BioRad).

    Article Title: Neutrophil- Leishmania infantum Interaction Induces Neutrophil Extracellular Traps, DAMPs, and Inflammatory Molecule Release.
    Article Snippet: RNA was collected from neutrophil cultures after 1, 4, or 24 h of infection, and RNA was collected in Trizol reagent (Invitrogen), followed by the extraction of total RNA, treatment with DNase, resuspension in nuclease-free water (Invitrogen), and quantification on a NanoDrop 2000 spectrophotometer (Thermo-Fisher, Waltham, Massachusetts). cDNA was generated using a high-capacity cDNA kit (Thermo-Fisher, Waltham, Massachusetts) according to the manufacturer’s instructions. qPCR reactions in 10 μL were performed on a QuantStudio 3 Real-Time PCR System (Thermo-Fisher, Waltham, Massachusetts).



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