lambda midi kit (Qiagen)

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Qiagen lambda midi kit
Lambda Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1469 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+lambda+system+kit/QIAGEN+Large-Construct+Kit/pmc03464210-222-6-9
Average 97 stars, based on 1469 article reviews

lambda midi kit - by Bioz Stars, 2026-11

97/100 stars

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BAC Assay:

Article Title: Zika virus produces two isoforms of the NS2A protein that play mutually exclusive roles in viral replication and virion assembly.
Article Snippet: .. Resulting ZIKVPR cDNA BAC were purified using the QIAGEN Large-Construct kit (Qiagen), and sequences were verified by Oxford Nanopore sequencing (Plasmidsaurus). ..

Article Title: Visualizing Changes in Brain-Derived Neurotrophic Factor Expression in Living Mice Using the All-Engineered Bioluminescence Imaging System AkaBLI.
Article Snippet: Brain-derived neurotrophic factor (BDNF) plays a pivotal role in neuronal development, synaptic plasticity, and cognitive function, and its dysregulation is implicated in various neurodegenerative and neuropsychiatric disorders.. To noninvasively monitor dynamic changes in Bdnf expression in vivo, we developed a novel transgenic mouse line, Bdnf-AkaLuc transgenic (Tg) mice, in which the coding region of BDNF was replaced in a BAC transgene with a mutant luciferase, AkaLuc.. This luciferase is optimized for the synthetic substrate AkaLumine, which emits near-infrared bioluminescence suitable for deeptissue imaging.

Article Title: The Y chromosome gene draupnir reveals constraints on engineering Y-linked sex-ratio distorters in malaria mosquitoes
Article Snippet: .. Two positive clones were identified, BAC-2C and BAC-5B, which were isolated using the QIAGEN Large-Construct kit (QIAGEN) for and sequenced using Single-Molecule Real-Time (SMRT) PacBio sequencing. ..

Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving 'chromosome'.
Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1, Sts, and Asmt genes. .. Since the mouse PAR is mostly GCrich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

Article Title: Comparative sequence analysis of the mouse pseudoautosomal region from three inbred strains reveals it to be the most rapidly evolving ‘chromosome’
Article Snippet: .. The BAC DNA was purified using QIAGEN Large-Construct Kit DNA sequencing by primer walking initially started from both ends of the BAC clone and from every exon of Mid1 , Sts , and Asmt genes. .. Since the mouse PAR is mostly GC-rich, we always used a 1:1 mixture of dGTP BigDye Terminator v3.0 (Applied Biosystems) and BigDye Terminator v3.1 Cycle Sequencing kits supplemented with dimethyl sulfoxide (final 10%(v/v)).

Purification:

Article Title: Synthesis and Biological Evaluation of Modified Peptide Derivatives Targeting the SARS-CoV‑2 Nsp3 Macrodomain (Mac1) Replication Domain
Article Snippet: .. Large-scale purification of the ΔS-Luc-GFP bacmid DNA was performed using the QIAGEN Large-Construct Kit (Cat. No. 12462) according to the manufacturer’s instructions. ..

Construct:

Article Title: Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolution
Article Snippet: Commercial assay or kit , Zymo DNA Clean & Concentrator Kit , Zymo Research , cat. #D4013 , . .. Commercial assay or kit , Large construct DNA isolation kit , Qiagen , cat. #12462 , . .. Commercial assay or kit , Qubit dsDNA HS Assay Kit , Thermo Fisher Scientific , cats. # Q32851 and # Q32856 , .

DNA Extraction:

Clone Assay:

Isolation:

PacBio Sequencing:

DNA Sequencing:

Chromosome Walking:

other:

Article Title: Baculoviruses exploit the mitotic kinase CDK1 to disrupt the nuclear lamina
Article Snippet: The resulting fragments were cloned into the pET-28a(+) vector (Novagen, Madison, USA), which contains an N-terminal 6 × His tag, to generate plasmids pET28a-His:lamin B (1–150) and pET28a-His:lamin B (1–150, S47A).

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