superscrip buffer (Thermo Fisher)
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Thermo Fisher
superscrip buffer
Superscrip Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscrip+buffer/FSB+buffer/us07393684-1807-17-19
Average 99 stars, based on 1 article reviews
Superscrip Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscrip+buffer/FSB+buffer/us07393684-1807-17-19
Average 99 stars, based on 1 article reviews
superscrip buffer - by Bioz Stars,
2026-09
99/100 stars
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Reverse Transcription:Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications. Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl Article Title: Multifaceted B cell response to transient HIV viremia in elite controllers. Article Snippet: .. Each well then received 5.4 μL of reverse transcription mix containing Superscript II reverse transcriptase (50 units) (Thermo Fisher), RNAse inhibitor (10 units) (Tarka Bio), Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Article Title: Altered placental expression of neurotransmitter and stress-related molecules in first-episode psychosis during pregnancy: Implications for cytoarchitecture and function. Article Snippet: .. A reverse transcription reaction mix was prepared, containing 2.8 μL of Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly. Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells Article Snippet: .. Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect Article Snippet: .. To this initial mixture, we added a 25 μl mixture of working concentrations of Incubation:Article Title: CD13 is a bona-fide marker of bovine pre-adipocytes with potential in cultivated fat applications. Article Snippet: RNA (300 ng) was incubated with 0.5 μl random primers (250ng/μl, Promega, 1181), 1 μl dNTPs (10mM, Invitrogen, 18427- 013) and nuclease free-water in a thermocycler (Biometra) at 65 °C for 5 min, followed by 5 min at 4 °C. .. Then, 1 μl DTT (0.1 M, Invitrogen, 18080-93), 1 μl RNasin Plus RNase inhibitor (40 U/μL, Promega, N2611), 1 μl SuperScript III reverse transcriptase (Invitrogen, 18080-93), and 4 μl Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS. Article Snippet: .. Cells were washed and resuspended in 500 μl Lysis:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Isolation:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × cDNA Synthesis:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × Recombinant:Article Title: cxcl18b -defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina Article Snippet: .. Interesting cells were collected into a 1.5 ml tube containing lysis buffer (20 mg/ml PK in TE buffer) and total RNA was isolated using this lysis buffer. cDNA synthesis was conducted by adding RT mix containing: 200 U Superscript II reverse transcriptase (Invitrogen, 18064‐014), 1 × FACS:Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS. Article Snippet: .. Cells were washed and resuspended in 500 μl Staining:Article Title: Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS. Article Snippet: .. Cells were washed and resuspended in 500 μl Concentration Assay:Article Title: Impaired function of Vγ9Vδ2 T cells in frail elderly. Article Snippet: .. The RT reaction mixture contained 0.5 μL SuperScript II reverse transcriptase (200 U/μL, Invitrogen), 0.25 μL RNase inhibitor (40 U/μL, Clontech), 2 μL 5× SuperScript II |