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tmb 1 component hrp microwell substrate  (ImmunoChemistry Technologies)


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    ImmunoChemistry Technologies tmb 1 component hrp microwell substrate
    Tmb 1 Component Hrp Microwell Substrate, supplied by ImmunoChemistry Technologies, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/substrates/TMB+1-Component+HRP+Microwell+Substrate/pmc12006086-60-3-8
    Average 93 stars, based on 6 article reviews
    tmb 1 component hrp microwell substrate - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin and Its Antithrombotic Activity
    Article Snippet: Cholesterol was from Sigma-Aldrich (St. Louis, USA). .. Antibody coating buffer, Neptune block, Neptune sample diluent, ELISA wash buffer, TMB 1-component HRP microwell substrate (SUBT) and stop solution for TMB substrates (STOPT) were from ImmunoChemistry Technologies, LLC (Bloomington, MN). .. The mouse anti-TM monoclonal antibody PBS-01 was from Abcam (Cambridge, MA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin and Its Antithrombotic Activity
    Article Snippet: Cholesterol was from Sigma-Aldrich (St. Louis, USA). .. Antibody coating buffer, Neptune block, Neptune sample diluent, ELISA wash buffer, TMB 1-component HRP microwell substrate (SUBT) and stop solution for TMB substrates (STOPT) were from ImmunoChemistry Technologies, LLC (Bloomington, MN). .. The mouse anti-TM monoclonal antibody PBS-01 was from Abcam (Cambridge, MA).

    Article Title: Synthesis of Hydrophobic and Antifouling Wood-Polymer Materials through SI-ATRP: Exploring a Versatile Pathway for Wood Functionalization
    Article Snippet: The biological activity of adsorbed IgG (not labelled) was evaluated through a binding assay employing goat anti-mouse IgG labelled with Alexa Fluor 488 (Invitrogen). .. For conducting ELISA tests to determine protein adsorption on wood cubes TMB (3,3’,5,5’-tetramethylbenzidine, ImmunoChemistry Technologies, 6276), 1M HCl (Sigma-Aldrich) and rabbit anti-goat IgG (H+L) HRP-labeled secondary antibody (Invitrogen) were used. .. The proteins were dissolved in phosphate-buffered saline (PBS, Merck).

    Incubation:

    Article Title: A selective C5a-derived peptidomimetic enhances IgG response following inactivated SARS-CoV-2 immunization and confers rapid disease resolution following murine coronavirus infection
    Article Snippet: .. 75 μL of TMB 1-Component HRP Microwell Substrate (ImmunoChemistry Technologies, cat# 6276) was added to each well and incubated for 10 minutes, followed by the addition of 75 μL of Stop Solution (ImmunoChemistry Technologies, cat# 6282) per well. .. Plates were gently tapped to ensure complete mixing, and absorbances were measured at 450 and 570 nm using a BioTek Synergy LX microplate reader (BioTek Instruments, cat# SLXFA).

    Article Title: Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1
    Article Snippet: After washing the plates and blocking any remaining binding sites with Starting Block (Pierce), the plates were incubated with LPL preparations for 2 h at 4°C. .. Wells were then washed with PBS/Mg/Ca containing 0.1% Tween-20, and LPL binding to antibody 5D2 was detected with a horseradish peroxidase (HRP)-labeled rabbit polyclonal antibody against the V5 tag (0.5 μg/ml, Abcam) or an HRP-labeled rabbit polyclonal antibody against the S-protein tag (0.5 μg/ml, Abcam) followed by an incubation with TMB 1-Component HRP Microwell Substrate (SUB1, ImmunoChemistry Technologies). .. Effect of furin inhibition on secretion of LPL from transfected cells CHO-K1 cells were electroporated with different LPL expression vectors and were then grown in a medium in the presence or the absence of 50 μ m decanoyl-RVKR-chloromethylketone (CMK, Calbiochem) for 24 h. Cell extracts and samples of conditioned medium were then analyzed by western blotting.

    Article Title: Development of a Multispecies Double-Antigen Sandwich ELISA Using N and RBD Proteins to Detect Antibodies against SARS-CoV-2
    Article Snippet: Subsequently, 50 μL of N and RBD proteins conjugated with peroxidase (N-HRP, RBD-HRP) were added to each plate as appropriate (i.e., plates coated with N protein were revealed with N-HRP) at the same concentration as the coated proteins and were incubated for 30 min at RT. .. Afterward, 50 μL of the 3,3′,5,5′-tetramethylbenzidine (TMB, 1-Component HRP Microwell Substrate, ImmunoChemistry Technologies) reagent was added and incubated for 20 min in darkness at RT. ..

    Binding Assay:

    Article Title: Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1
    Article Snippet: After washing the plates and blocking any remaining binding sites with Starting Block (Pierce), the plates were incubated with LPL preparations for 2 h at 4°C. .. Wells were then washed with PBS/Mg/Ca containing 0.1% Tween-20, and LPL binding to antibody 5D2 was detected with a horseradish peroxidase (HRP)-labeled rabbit polyclonal antibody against the V5 tag (0.5 μg/ml, Abcam) or an HRP-labeled rabbit polyclonal antibody against the S-protein tag (0.5 μg/ml, Abcam) followed by an incubation with TMB 1-Component HRP Microwell Substrate (SUB1, ImmunoChemistry Technologies). .. Effect of furin inhibition on secretion of LPL from transfected cells CHO-K1 cells were electroporated with different LPL expression vectors and were then grown in a medium in the presence or the absence of 50 μ m decanoyl-RVKR-chloromethylketone (CMK, Calbiochem) for 24 h. Cell extracts and samples of conditioned medium were then analyzed by western blotting.

    Adsorption:

    Article Title: Synthesis of Hydrophobic and Antifouling Wood-Polymer Materials through SI-ATRP: Exploring a Versatile Pathway for Wood Functionalization
    Article Snippet: The biological activity of adsorbed IgG (not labelled) was evaluated through a binding assay employing goat anti-mouse IgG labelled with Alexa Fluor 488 (Invitrogen). .. For conducting ELISA tests to determine protein adsorption on wood cubes TMB (3,3’,5,5’-tetramethylbenzidine, ImmunoChemistry Technologies, 6276), 1M HCl (Sigma-Aldrich) and rabbit anti-goat IgG (H+L) HRP-labeled secondary antibody (Invitrogen) were used. .. The proteins were dissolved in phosphate-buffered saline (PBS, Merck).



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    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and <t>AS160</t> phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 <t>S318,</t> and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
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    Image Search Results


    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Journal: Journal of Sport and Health Science

    Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice

    doi: 10.1016/j.jshs.2025.101100

    Figure Lengend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Article Snippet: Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Akt substrate 160 (AS160 S318; #8619; Cell Signaling Technologies), AS160 T642 (#8881; Cell Signaling Technologies), eukaryotic translation initiation factor 4E binding protein (4E-BP1; #9452; Cell Signaling Technologies), and glyceraldehyde 3 phosphate dehydrogenase (GAPDH; #2118; Cell Signaling Technologies).

    Techniques: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay