akt substrate 160 (Cell Signaling Technology Inc)
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akt substrate 160
Akt Substrate 160, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/substrate/Phospho-AS160+(Ser318)+Rabbit+mAb/pmc12816905-99-63-69
Average 93 stars, based on 31 article reviews
Akt Substrate 160, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/substrate/Phospho-AS160+(Ser318)+Rabbit+mAb/pmc12816905-99-63-69
Average 93 stars, based on 31 article reviews
akt substrate 160 - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice"
Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice
Journal: Journal of Sport and Health Science
doi: 10.1016/j.jshs.2025.101100
Figure Legend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
Techniques Used: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay
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Molecular Weight:Article Title: Liver acts as a metabolic gate for the traumatic brain injury pathology: Protective action of thyroid hormone Article Snippet: Bound antibody was visualized using HRP conjugated secondary antibody (1 h at room temperature) and membrane was developed using gel documentation instrument (Bio-Rad, USA) ( ). .. Antibody Company Catalog No. Molecular Weight (kDa) pAMPKα (thr 172) Cell signaling 2535 62 AMPKα Cell signaling 2603 62 pACC Cell signaling 3661 280 ACC Cell signaling 3662 280 pAKT (ser 473) Cell signaling 4060 60 AKT Cell signaling 4691 60 pGSK3β (ser 9) Cell signaling 9336 46 GSK3β Cell signaling 12456 46 FAS Cell signaling 3180 273 AceCS1 Cell signaling 3658 78 other:Article Title: Liver acts as a metabolic gate for the traumatic brain injury pathology: Protective action of thyroid hormone Article Snippet: Ubiquitin Proteomics:Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice Article Snippet: .. Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice. Article Snippet: .. Standard insulin treatment and immunoblotting procedures were performed as previously described.26,30 The plantaris was chosen based on previous studies showing efficacious response to exercise training as well as insulin response.30 Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4 (COX4; #11967; Cell Signaling Technologies), Binding Assay:Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice Article Snippet: .. Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice. Article Snippet: .. Standard insulin treatment and immunoblotting procedures were performed as previously described.26,30 The plantaris was chosen based on previous studies showing efficacious response to exercise training as well as insulin response.30 Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4 (COX4; #11967; Cell Signaling Technologies), Western Blot:Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice. Article Snippet: .. Standard insulin treatment and immunoblotting procedures were performed as previously described.26,30 The plantaris was chosen based on previous studies showing efficacious response to exercise training as well as insulin response.30 Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4 (COX4; #11967; Cell Signaling Technologies), |