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one component tmb substrate solution  (Elabscience Biotechnology)


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    Elabscience Biotechnology one component tmb substrate solution
    One Component Tmb Substrate Solution, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/substrate/One-component+TMB+Substrate/pmc13030106-150-2-7
    Average 94 stars, based on 20 article reviews
    one component tmb substrate solution - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Designing multifunctional recombinant vaccines: an engineering strategy based on innovative epitope prediction-guided splicing.
    Article Snippet: .. After incubation, the plate was washed three times with PBS and incubated with HRP-goat anti-mouse IgG (Trans, HS201-01) for 1 h. Following another wash cycle with PBS three times, TMB Substrate (Elabscience, E-IR-R201) was added to initiate the reaction. ..

    Article Title: Epidemiological Survey of Porcine Circovirus Types 2 and 3 in Liaoning Region of China and Preparation of Monoclonal Antibodies Against PCV3 Cap Protein.
    Article Snippet: .. After washing, one-component TMB substrate solution (E-IR-R201; Elabscience, Wuhan, China) was added and incubated at 37 ◦C in the dark, and the reaction was terminated by the addition of 50 μL stop solution (E-ELIR-006; Elabscience, Wuhan, China). .. OD450 values were measured using a microplate reader (Infinite F200; Tecan, Männedorf, Switzerland).

    Article Title: Epidemiological Survey of Porcine Circovirus Types 2 and 3 in Liaoning Region of China and Preparation of Monoclonal Antibodies Against PCV3 Cap Protein
    Article Snippet: .. After washing, one-component TMB substrate solution (E-IR-R201; Elabscience, Wuhan, China) was added and incubated at 37 °C in the dark, and the reaction was terminated by the addition of 50 μL stop solution (E-ELIR-006; Elabscience, Wuhan, China). .. OD 450 values were measured using a microplate reader (Infinite F200; Tecan, Männedorf, Switzerland).

    Selection:

    Article Title: Neutralization of Crimean-Congo hemorrhagic fever pseudotyped virions with heavy chain antibodies.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Neutralization of Crimean-Congo hemorrhagic fever pseudotyped virions with heavy chain antibodies
    Article Snippet: .. After three rounds of selection, individual colonies were screened by ELISA using HRP-labeled M13 monoclonal antibody (Catalog No. 1973-MM05T-H, Sino Biological, Beijing, China) for detection, with tetramethylbenzidine (TMB; Catalog No. E-IR-R201, Elabscience, Wuhan, China) developed for 20 min before stopping with 50 μL stop solution (Catalog No. C1058, Solarbio, Beijing, China). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neutralization of Crimean-Congo hemorrhagic fever pseudotyped virions with heavy chain antibodies.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Neutralization of Crimean-Congo hemorrhagic fever pseudotyped virions with heavy chain antibodies
    Article Snippet: .. After three rounds of selection, individual colonies were screened by ELISA using HRP-labeled M13 monoclonal antibody (Catalog No. 1973-MM05T-H, Sino Biological, Beijing, China) for detection, with tetramethylbenzidine (TMB; Catalog No. E-IR-R201, Elabscience, Wuhan, China) developed for 20 min before stopping with 50 μL stop solution (Catalog No. C1058, Solarbio, Beijing, China). ..

    other:

    Article Title: A novel dual-effect bimodal chip cancer research platform: Chips system interconnected vascularized tumor organoids culture with real-time exploration and detection from bench to bedside.
    Article Snippet: 23 Nowadays, cancer researches widely employed organoids as research model. Organoids 24 provide a realistic applicate scene but how to real-time detect and quantified the organoid 25 status is still a bottleneck.. To bridge this knowledge gap, we developed an all-in-one 26 microfluidic platform that, for the first time, seamlessly integrates a vascularized tumor 27 organoids-on-a-chip model (VOoC) with a real-time ELISA detection module for advanced 28 tumor research and point of care testing (POCT).. The platform features a unique integration 29 of two cutting-edge technologies: (1) VOoC consists of a perfusable self-forming vessel 30 network throughout the tumor organoid, emulates in vivo tumor angiogenesis, and (2) a real31 time microfluidic diagnostic chip equipped with ELISA for simultaneously monitoring of 32 tumor biomarker expression levels.



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    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and <t>AS160</t> phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 <t>S318,</t> and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
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    Image Search Results


    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Journal: Journal of Sport and Health Science

    Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice

    doi: 10.1016/j.jshs.2025.101100

    Figure Lengend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Article Snippet: Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Akt substrate 160 (AS160 S318; #8619; Cell Signaling Technologies), AS160 T642 (#8881; Cell Signaling Technologies), eukaryotic translation initiation factor 4E binding protein (4E-BP1; #9452; Cell Signaling Technologies), and glyceraldehyde 3 phosphate dehydrogenase (GAPDH; #2118; Cell Signaling Technologies).

    Techniques: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay