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rneasy total rna kit (Qiagen)

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Qiagen rneasy total rna kit

FIGURE 1. Der f dose dependently induces the expression of proin- ﬂammatory mediators of AMs. AMs were stimulated for 24 h with a non- cytotoxic dose of Der f, OVA, or LPS. The accumulation of proinﬂam- matory mediators in supernatants was evaluated (see Materials and Methods). Data are the means SEM of three to six separate experiments performed in triplicate. , p 0.05 compared with medium control (A). <t>Total</t> <t>RNA</t> extracted from stimulated AMs was evaluated by RT-PCR. A representative RT-PCR proﬁle from three independent experiments is shown (B). Nuclear proteins were extracted from AMs 24 h after incuba- tion with Der f, and activation of NF-B was evaluated by EMSA. Super- shift was performed using Abs to the p50 (p50) and p65 (p65) subunits of NF-B. Unlabeled oligonucleotides containing binding sites for NF-B (B), AP-1, and speciﬁcity protein 1 were used for competition. Figure is representative of three independent experiments (C).

Rneasy Total Rna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stratascript+h-reverse+transcriptase/pm12496440-96-9-13?v=Qiagen
Average 99 stars, based on 1969 article reviews

rneasy total rna kit - by Bioz Stars, 2026-07

99/100 stars

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1) Product Images from "House dust mite Dermatophagoides farinae augments proinflammatory mediator productions and accessory function of alveolar macrophages: implications for allergic sensitization and inflammation."

Article Title: House dust mite Dermatophagoides farinae augments proinflammatory mediator productions and accessory function of alveolar macrophages: implications for allergic sensitization and inflammation.

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.170.1.528

Figure Legend Snippet: FIGURE 1. Der f dose dependently induces the expression of proin- ﬂammatory mediators of AMs. AMs were stimulated for 24 h with a non- cytotoxic dose of Der f, OVA, or LPS. The accumulation of proinﬂam- matory mediators in supernatants was evaluated (see Materials and Methods). Data are the means SEM of three to six separate experiments performed in triplicate. , p 0.05 compared with medium control (A). Total RNA extracted from stimulated AMs was evaluated by RT-PCR. A representative RT-PCR proﬁle from three independent experiments is shown (B). Nuclear proteins were extracted from AMs 24 h after incuba- tion with Der f, and activation of NF-B was evaluated by EMSA. Super- shift was performed using Abs to the p50 (p50) and p65 (p65) subunits of NF-B. Unlabeled oligonucleotides containing binding sites for NF-B (B), AP-1, and speciﬁcity protein 1 were used for competition. Figure is representative of three independent experiments (C).

Techniques Used: Expressing, Control, Reverse Transcription Polymerase Chain Reaction, Activation Assay, Binding Assay

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rneasy total rna kit (Qiagen)

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1) Product Images from "House dust mite Dermatophagoides farinae augments proinflammatory mediator productions and accessory function of alveolar macrophages: implications for allergic sensitization and inflammation."

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