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Thermo Fisher strand buffer

a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release <t>mRNAs.</t> <t>First-strand</t> cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strand+buffer/BETAINE/pmc13009247-443-26-36
Average 96 stars, based on 1 article reviews

strand buffer - by Bioz Stars, 2026-11

96/100 stars

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1) Product Images from "hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development"

Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development

Journal: Nature Communications

doi: 10.1038/s41467-026-69176-8

a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

Figure Legend Snippet: a Schematic illustration of the SCAN-seq method. Briefly, control and Hnrnpm cKO GV oocytes were lysed to release mRNAs. First-strand cDNAs were synthesized using a 24-nt barcode RT primer. Following amplification, the cDNAs derived from multiple cells were combined, pooled together, and purified. Subsequently, the full-length cDNAs were used for the construction of nanopore libraries and sequencing. The mouse, oocyte, and nucleic acid schematics are modified adaptations of original resources from SciDraw and Servier Medical Art, respectively, and are employed under a CC BY 4.0 license. b – j PCR and Sanger sequencing validation of genes ( Nlrp14, Meikin, Vrk1 , and Zar1l ) with novel isoforms identified in GV oocytes. Ref. Reference. Representative results shown in ( b – e ) were obtained from at least three independent experiments with similar results. Source data ( b – e ) are provided as a Source data file.

Techniques Used: Control, Synthesized, Amplification, Derivative Assay, Purification, Sequencing, Modification, Biomarker Discovery

Concentration Assay:

Article Title: Array and method for detecting spatial information of nucleic acids
Article Snippet: .. Final Ingredient Volume (ul) concentration Superscript II First strand buffer 40 1× (5×), Thermo Fisher Betaine (5M), Aladdin 40 1M dNTP (10 mM), Thermo Fisher 20 1 mM MgCl2 (100 mM), Aladdin 15 7.5 mM TSO sequence (50 uM), synthesized 10 1 uM by Beijing Liuhe BGI Superscript II RT (200 U/ul), 10 10 U/ul Thermo Fisher DTT (100 mM) 10 5 mM RNase inhibitor (40 U/ul), 5 1 u/ul Thermo Fisher NF H2O 50 4. ..

Article Title: Array and method for detecting spatial information of nucleic acids
Article Snippet: .. Final Ingredient Volume (ul) concentration Superscript II First strand buffer 40 1× (5×), Thermo Fisher Betaine (5M), Aladdin 40 1M dNTP (10 mM), Thermo Fisher 20 1 mM MgCl2 (100 mM), Aladdin 15 7.5 mM TSO sequence (50 uM), synthesized 10 1 uM by Beijing Liuhe BGI Superscript II RT (200 U/ul), 10 10 U/ul Thermo Fisher DTT(100 mM) 10 5 mM RNase inhibitor (40 U/ul), 5 1 u/ul Thermo Fisher Nucleic acid-free molecular water 50 (NF H2O) 4. ..

Sequencing:

Article Title: Array and method for detecting spatial information of nucleic acids
Article Snippet: .. Final Ingredient Volume (ul) concentration Superscript II First strand buffer 40 1× (5×), Thermo Fisher Betaine (5M), Aladdin 40 1M dNTP (10 mM), Thermo Fisher 20 1 mM MgCl2 (100 mM), Aladdin 15 7.5 mM TSO sequence (50 uM), synthesized 10 1 uM by Beijing Liuhe BGI Superscript II RT (200 U/ul), 10 10 U/ul Thermo Fisher DTT(100 mM) 10 5 mM RNase inhibitor (40 U/ul), 5 1 u/ul Thermo Fisher Nucleic acid-free molecular water 50 (NF H2O) 4. ..

Synthesized:

Article Title: Array and method for detecting spatial information of nucleic acids
Article Snippet: .. Final Ingredient Volume (ul) concentration Superscript II First strand buffer 40 1× (5×), Thermo Fisher Betaine (5M), Aladdin 40 1M dNTP (10 mM), Thermo Fisher 20 1 mM MgCl2 (100 mM), Aladdin 15 7.5 mM TSO sequence (50 uM), synthesized 10 1 uM by Beijing Liuhe BGI Superscript II RT (200 U/ul), 10 10 U/ul Thermo Fisher DTT(100 mM) 10 5 mM RNase inhibitor (40 U/ul), 5 1 u/ul Thermo Fisher Nucleic acid-free molecular water 50 (NF H2O) 4. ..

Reverse Transcription:

Article Title: Neocortical temporal patterning by a two-layered regulatory network
Article Snippet: Briefly, RNA was eluted into 4 μL elution mix containing 1 μL RT primer (10 μM), 1 μL dNTP mix (10 mM each, New England Biolabs, N0447L), 1 μL RNase Inhibitor (4 U/μL), and 1 μL H 2 O. Eluted samples were incubated at 72 °C for 3 min and immediately placed on ice. .. Each sample was added with 7 μL reverse transcription (RT) mix containing 0.75 μL H 2 O, 0.1 μL Maxima Reverse Transcriptase (Thermo Fisher Scientific, EP0741), 2 μL 5 × RT buffer (Thermo Fisher Scientific, EP0741), 2 μL Betaine (5 M, Sigma-Aldrich, B0300), 0.9 μL MgCl 2 (100 mM), 1 μL TSO primer (10 μM), 0.25 μL RNase Inhibitor (40 U/μL). ..

Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development
Article Snippet: .. Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers. ..

Article Title: hnRNPM cooperates with BCAS2 to modulate alternative splicing during oocyte development.
Article Snippet: .. Then, each sample was mixed with 2.83 μL of reverse transcription mixture, comprising SuperScript IV reverse transcriptase (Invitrogen, 18090050), RNase inhibitor (Takara, Cat. 2313A), SuperScript IV first-strand buffer, 1 M betaine (Sigma-Aldrich, B0300-1VL), 100 mM DTT (Invitrogen, 18090050), 50 mM MgCl2 (Sangon, A610328-0500), and template-switching oligo primers. ..

other:

Article Title: ThermiQuant™ AquaStream: A Portable Instrument for Quantitative Colorimetric Isothermal Nucleic Acid Amplification Reactions in Paper and Tube Formats
Article Snippet: For the preparation of 200 μL of the final LAMP master mix, 125 μL of the 2× LAMP mix was combined with 25 μL of 10× primer mix (16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LF/LB; final concentrations: 1.6 μM FIP/BIP, 0.2 μM F3/B3, 0.4 μM LF/LB; see Table S4), 0.67 μL Bst 2.0 DNA polymerase, 1 μL betaine (5 M; Sigma-Aldrich, B03005VL), 3.13 μL bovine serum albumin (BSA) (40 mg/mL; Sigma-Aldrich, A2153), 36.0 μL trehalose (1.75 M; Thermo Scientific Chemicals, 182550250), and 9.2 μL nuclease-free water.

Article Title: MEN1 Promotes Ferroptosis by Disrupting CD44 Alternative Splicing to Suppress Lung Cancer
Article Snippet: Tumors and normal tissues in different regions were isolated by using MMI CellCut laser microdissection (Molecular Machines & Industries) and collected into sterile IsolationCaps.

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