first strand buffer (Thermo Fisher)

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Thermo Fisher first strand buffer
First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/strand+buffer/FSB+Buffer/pmc12857400-346-35-40
Average 94 stars, based on 1 article reviews

first strand buffer - by Bioz Stars, 2026-11

94/100 stars

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Saline:

Article Title: Consequences of irradiation on blood-brain tumor barrier model of Diffuse Midline Glioma: characterization of physical and metabolic properties.
Article Snippet: .. After several rinses with Phosphate-Buffered Saline Calcium and Magnesium Free (PBS-CMF; 136 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8mM Na2PO4 12H2O), pericytes were gently scraped off the filter and non-specific sites were blocked with pure Fish Serum Blocking buffer (SBB, 37527, Thermo Scientific, Rockford, USA). .. For ECs staining, the filters were incubated with primary antibody solution for 1 h (diluted in PBS-CMF containing 5% SBB) for ZO-1 (rabbit anti-ZO-1, 61-7300, Invitrogen), VE-Cadherin (rabbit anti-VE-cadherin, ab33168, Abcam), Claudin-5 (rabbit anti-CLDN-5, 34-1600, Invitrogen), phospho-Histone H2A.X (mouse anti-phosphoHistone H2A.X (Ser139) monoclonal antibody (3F2), MA1-2022, Invitrogen).

Blocking Assay:

Amplification:

Article Title: Nucleotide analogues
Article Snippet: “Phospho” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and Compound C. “Fluoro” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and Compound B. .. “Hydroxy” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and Compound A. .. “Commercial Inhibitor” This lane was loaded with the amplification product obtained from amplifying genomic RNA from the 2019 Novel Coronavirus; Strain: 2019-nCoV/USA-WA1/2020 (Reference: ATCC-VR-1986D, ATCC), using the NSP12/RdRp catalytic domain of RNA-directed RNA polymerase (Reference: PX-COV-P006, ProteoGenix) of SARS-CoV2 and the RNA primers (same as the lane “RNA Primer”), using the 10× First Strand Buffer and RNase Inhibitor from Amino Allyl MessageAmpTM II aRNA Amplification Kit (Reference: AM1753, Thermo), and rNTPs, in equimolar amounts, except that the standard amount for rATP was split 50:50 between rATP and the commercial inhibitor.

Incubation:

Article Title: Pharmacologic Modulation of ARID3A with Rimegepant Reactivates Type I Interferon Signaling and Sensitizes Triple-Negative Breast Cancer to PD-1 Blockade.
Article Snippet: Cell viability was assessed using the Fixable iability Dye Red 780 (Elabscience, Cat# E-CK-A168; displayed as LD R780” in all flow cytometry plots). .. In selected experiments, ell suspensions from EO771-OVA models were incubated with E-labeled H-2Kb OVA (SIINFEKL) tetramer (Yeasen Biotechnolgy, Cat# 13903) for 30 min at room temperature in the dark prior o surface antibody staining. or intracellular cytokine staining (ICS), single-cell suspensions ere stimulated with PMA and ionomycin in the presence of rotein transport inhibitors (e.g., Brefeldin A) prior to fixation nd permeabilization using the Foxp3/Transcription Factor Stainng Buffer Set (eBioscience). .. Cells were subsequently stained ith antibodies against IFN- γ (APC, BioLegend, Clone XMG1.2, at# 505810), Granzyme B (PerCP/Cyanine5.5, BioLegend, Clone B11, Cat# 372212), TNF- α (Brilliant Violet 510, BioLegend, Clone P6-XT22, Cat# 506339), Ki-67 (Brilliant Violet 605, BioLeend, Clone 16A8, Cat# 652413), and FOXP3 (Alexa Fluor 700, Bioscience, Clone 150D, Cat# 126422). ata acquisition was performed based on assay complexity: highimensional multicolor analyses of tumor-infiltrating immune dvanced Science, 2026 cells were acquired on a BD LSRFortessa flow cytometer, whereas selected low-parameter in vitro assays were acquired on a BD Accuri C6 flow cytometer.

Article Title: Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed.
Article Snippet: .. Generation and circularization of single-stranded DNA (ssDNA) from isolated RNA footprints Ligated footprints were incubated with dNTP mix, 12.5 μM linker RT, FSB buffer (Invitrogen), murine RNAse inhibitor (New England Biolabs), 0.01 M DTT and 2 μl of Superscript III (Invitrogen) for 30 min at 50 °C. .. The reaction was terminated by the addition of 1 N NaOH (Ambion).

Staining:

Single Cell:

Isolation:

SYBR Green Assay:

Article Title: Antimicrobial resistance genes in wild coyotes ( Canis latrans ) from Guanacaste and Central conservation areas of Costa Rica
Article Snippet: ARGs were screened using quantitative PCR (qPCR) on a CFX96 Real-Time System (Bio-Rad, USA). .. The reaction mixture consisted of 5 μL of PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific), 0.4 μL of each forward and reverse primer (10 μM) (Invitrogen), 0.25 μL of Yellow Sample Buffer (Thermo Fisher Scientific), 2.95 μL of RNase-free water, and 1 μL of template DNA, for a final volume of 10 μL. ..

other:

Article Title: Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapy
Article Snippet: Then, RNA extraction was performed with the RNeasy Mini Kit (Qiagen).

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