Review



nebnext second strand synthesis reaction buffer  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    New England Biolabs nebnext second strand synthesis reaction buffer
    Nebnext Second Strand Synthesis Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/NEBNext+Second+Strand+Synthesis+(dNTP-free)+Reaction+Buffer/pm41590694-83-57-57
    Average 98 stars, based on 800 article reviews
    nebnext second strand synthesis reaction buffer - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Artificial Neural Network Elucidates the Role of Transport Proteins in Rhodopseudomonas palustris CGA009 During Lignin Breakdown Product Catabolism.
    Article Snippet: .. Subsequently, to perform first-strand cDNA synthesis, 8 μL of NEBNext Strand Specificity Reagent and https://doi.org/10.3390/metabo16010086 2 μL of NEBNext First Strand Synthesis Enzyme Mix was added to the reaction and the reaction was incubated at 25 ◦C 10 min, 42 ◦C 30 min, 70 ◦C 15 min. Second-strand reaction was then performed by adding 8 μL of NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix (10×), 4 μL of NEBNext Second Strand Synthesis Enzyme Mix, and 48 μL of nuclease-free water. .. The reaction was incubated at 16 ◦C 1 h. The cDNA was purified by 1.8× SPRIselect Beads (Beckman Coulter) and eluted in 50 μL of nuclease-free water.

    Article Title: Artificial Neural Network Elucidates the Role of Transport Proteins in Rhodopseudomonas palustris CGA009 During Lignin Breakdown Product Catabolism
    Article Snippet: .. Subsequently, to perform first-strand cDNA synthesis, 8 μL of NEBNext Strand Specificity Reagent and 2 μL of NEBNext First Strand Synthesis Enzyme Mix was added to the reaction and the reaction was incubated at 25 °C 10 min, 42 °C 30 min, 70 °C 15 min. Second-strand reaction was then performed by adding 8 μL of NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix (10×), 4 μL of NEBNext Second Strand Synthesis Enzyme Mix, and 48 μL of nuclease-free water. .. The reaction was incubated at 16 °C 1 h. The cDNA was purified by 1.8× SPRIselect Beads (Beckman Coulter) and eluted in 50 μL of nuclease-free water.

    Incubation:

    Article Title: Artificial Neural Network Elucidates the Role of Transport Proteins in Rhodopseudomonas palustris CGA009 During Lignin Breakdown Product Catabolism.
    Article Snippet: .. Subsequently, to perform first-strand cDNA synthesis, 8 μL of NEBNext Strand Specificity Reagent and https://doi.org/10.3390/metabo16010086 2 μL of NEBNext First Strand Synthesis Enzyme Mix was added to the reaction and the reaction was incubated at 25 ◦C 10 min, 42 ◦C 30 min, 70 ◦C 15 min. Second-strand reaction was then performed by adding 8 μL of NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix (10×), 4 μL of NEBNext Second Strand Synthesis Enzyme Mix, and 48 μL of nuclease-free water. .. The reaction was incubated at 16 ◦C 1 h. The cDNA was purified by 1.8× SPRIselect Beads (Beckman Coulter) and eluted in 50 μL of nuclease-free water.

    Article Title: Artificial Neural Network Elucidates the Role of Transport Proteins in Rhodopseudomonas palustris CGA009 During Lignin Breakdown Product Catabolism
    Article Snippet: .. Subsequently, to perform first-strand cDNA synthesis, 8 μL of NEBNext Strand Specificity Reagent and 2 μL of NEBNext First Strand Synthesis Enzyme Mix was added to the reaction and the reaction was incubated at 25 °C 10 min, 42 °C 30 min, 70 °C 15 min. Second-strand reaction was then performed by adding 8 μL of NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix (10×), 4 μL of NEBNext Second Strand Synthesis Enzyme Mix, and 48 μL of nuclease-free water. .. The reaction was incubated at 16 °C 1 h. The cDNA was purified by 1.8× SPRIselect Beads (Beckman Coulter) and eluted in 50 μL of nuclease-free water.

    Nick Translation:

    Article Title: Chromatin crosstalk between HDA19 and NuA4 sets thresholds for stress gene activation in Arabidopsis
    Article Snippet: First, reverse transcription reaction was carried out with barcoded and UMI-containing oligo(dT) primers and SuperScript III (Thermo Fisher Scientific, Cat. No 12574026). cDNA was pooled and next purified with AMPure beads (Beckman Coulter, Cat. No. A63881). .. A second strand synthesis was performed overnight with the nick translation reaction containing 1x NEBNext Second Strand Synthesis Reaction Buffer (New England Biolabs, Cat. No. B6117S), 1 U RNase H (New England Biolabs, Cat. No. M0297L), 1 U E . coli DNA ligase I (New England Biolabs, Cat. No. M0205L), 5 U E . coli DNA polymerase (New England Biolabs, Cat. No. M0209L) and 30 μM dNTPs. ..



    Similar Products

    95
    ABclonal Biotechnology abclonal first strand synthesis buffer
    Abclonal First Strand Synthesis Buffer, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/5X+First-Strand+Buffer/pm42514934-89-45-45
    Average 95 stars, based on 1 article reviews
    abclonal first strand synthesis buffer - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    ABclonal Biotechnology first strand synthesis reaction buffer
    First Strand Synthesis Reaction Buffer, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/5X+First-Strand+Buffer/pm42462662-64-26-25
    Average 95 stars, based on 1 article reviews
    first strand synthesis reaction buffer - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    98
    ABclonal Biotechnology bodipytm 493 503 invitrogen d3922 cell lysis buffer
    Bodipytm 493 503 Invitrogen D3922 Cell Lysis Buffer, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/ABscript+II+cDNA+First+Strand+Synthesis+kit/pm42087801-322-23-17
    Average 98 stars, based on 1 article reviews
    bodipytm 493 503 invitrogen d3922 cell lysis buffer - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    95
    Thermo Fisher 1x first strand buffer
    1x First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/Tris+EDTA+buffer%2C+for+molecular+biology%2C+DNAse%2C+RNAse%2C+Protease+free+ready+to+use%2C+pH+8%2E0/pm42002808-95-17-21
    Average 95 stars, based on 1 article reviews
    1x first strand buffer - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher first strand buffer
    First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/FSB+Buffer/us12582656-115-51-67
    Average 94 stars, based on 1 article reviews
    first strand buffer - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    96
    Thermo Fisher 5× first strand buffer
    RT mix-II
    5× First Strand Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strand+buffer/FSB+buffer/pmc12765176-63-0-11
    Average 96 stars, based on 1 article reviews
    5× first strand buffer - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Journal: STAR Protocols

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells

    doi: 10.1016/j.xpro.2025.104275

    Figure Lengend Snippet: RT mix-II

    Article Snippet: 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044.

    Techniques: