ky626045 strain ma5  (Nobilis Therapeutics)

Journal: Synthetic and Systems Biotechnology

Article Title: Metabolic engineering of Saccharomyces cerevisiae for co-production of ergothioneine, salidroside and gadusol

doi: 10.1016/j.synbio.2026.04.010

Figure Lengend Snippet: Multicopy integration enhances EGT production. (A) Schematic of the multicopy integration strategy. (B) EGT titer of strains after Gene 1 integration. (C) EGT titer upon multicopy integration of Gene 2 into the E68 strain. (D) Fed-batch fermentation of strain E103 was performed in a 5-L bioreactor. Intracellular: intracellular EGT content, Extracellular: extracellular EGT content, Ethanol: ethanol content in the culture medium, Glucose: Glucose content in the culture medium. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Article Snippet: Transformants were randomly selected from selective plates for fermentation validation, yielding strain E68, which achieved an EGT titer of 50 mg L −1 ( B).

Techniques:

Journal: Journal of Immunology Research

Article Title: Pdcd1 −/− Mice Exhibit High Susceptibility to Respiratory Syncytial Virus Infection

doi: 10.1155/jimr/1223280

Figure Lengend Snippet: Pdcd1 −/− mice are susceptible to intranasal RSV infection. Mice were inoculated intranasally with 10 6 PFU of purified RSV A2 strain. (A) Body weight was monitored daily for 7 dpi. At the time of inoculation (0 dpi), the body weights of mice were set as the baseline, and subsequent weight changes relative to the baseline are shown ( n = 3–5 mice per group). (B) Viral loads in the lungs of RSV‐infected mice were measured at the indicated time points. n = 5 mice per group. (C) Lungs were collected on 0, 4, and 7 dpi and subjected to H&E staining. Scale bars, 50 μm. (D) Statistical analysis based on histological scores for perivascular and alveolar wall inflammatory cell infiltration. n = 5 mice per group. All data are presented as mean ± SD. Statistical significance was determined by two‐way ANOVA with Tukey’s post hoc test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001 versus WT mice; ns, not significant.

Article Snippet: RSV strains long (ATCC VR‐26) and A2 (ATCC VR‐1540) were used for mouse challenge experiments.

Techniques: Infection, Purification, Staining, Analysis

Journal: Chemistry & Biodiversity

Article Title: Green Acid Mediated Synthesis of Imidazo[1,2‐a]Pyrimidine–Thiazole/1,3,4‐Thiadiazole Hybrids: Design, Characterization and In‐Vitro Biological Evaluation

doi: 10.1002/cbdv.71857

Figure Lengend Snippet: Antibacterial activity of compounds B1–B37 (zone of inhibition, mm).

Article Snippet: All target molecules (B1–B37) were tested for antibacterial activity through the zone of inhibition method against four bacterial types: two Gram‐negative strains, Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853), and two Gram‐positive strains, Staphylococcus aureus (ATCC 23235) and Bacillus subtilis (ATCC 6051).

Techniques: Activity Assay, Inhibition, Bacteria