Lysis:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Extraction:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Modification:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Article Title: Enzyme‐Linked Cycloaddition Assay (ELCA) for Rapid, Ultra‐Sensitive Monitoring of Secreted Sialoglycoproteins
Article Snippet: A375 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with high glucose, 4.0 m m L‐glutamine, and sodium pyruvate (SH30243.01, Cytiva). .. THP‐1 cells (TIB‐202, ATCC) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (ATCC modification; A1049101, Gibco). .. The respective growth media were supplemented with 10% (v/v) heat‐inactivated fetal bovine serum (FBS; FBS‐HI‐12A, Capricorn Scientific) and 1% (v/v) penicillin‐streptomycin solution (P4333‐100ML, Sigma‐Aldrich).
Article Title: PLK1 inhibition by onvansertib enhances paclitaxel efficacy and overcomes resistance in HR+/HER2- breast cancer refractory to endocrine therapy and CDK4/6 inhibitors
Article Snippet: .. MCF7 cells were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; 118 ATCC, Cat. No. 30-2002) supplemented with 10% fetal bovine serum (FBS; ATCC, Cat. No. 30-119 2020), while all other parental cell lines were maintained in RPMI-1640 medium (ATCC, Cat. No. 120 30-2001) with 10% FBS. .. The fulvestrant-resistant MCF7 cell line, MCF7/164R-7 (MCF7/FR; 121 CancerTools, Cat. No. 152103; RRID: CVCL_1D39), was cultured in phenol red–free DMEM/F12 122 (Gibco, Cat. No. 11039021) supplemented with 1% FBS and 100 nM fulvestrant (Tocris, Cat. No. 123 1047).
Recombinant:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Western Blot:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Transfection:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Membrane:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Tissue:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Protease Inhibitor:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
SYBR Green Assay:Article Title: The COX2-PGE 2 -PKA axis suppresses antiviral immunity by inhibiting mtDNA-dependent STING activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies STING (D2P2F) Rabbit mAb Cell Signaling Technology Cat#13647; RRID: AB_2732796 Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit mAb Cell Signaling Technology Cat#5483, RRID: AB_10693472 TBK1/NAK (D1B4) Rabbit mAb Cell Signaling Technology Cat#3504; RRID: AB_2255663 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell Signaling Technology Cat#4947; RRID: AB_823547 IRF-3 (D83B9) Rabbit mAb Cell Signaling Technology Cat#4302; RRID: AB_1904036 GAPDH (14C10) Rabbit mAb Cell Signaling Technology Cat#2118; RRID: AB_561053 TFAM (D5C8) Rabbit mAb Cell Signaling Technology Cat#8076; RRID: AB_10949110 HSP90 Antibody Cell Signaling Technology Cat#4874; RRID: AB_2121214 PINK1 Antibody - BSA Free Novus Biologicals Cat#BC100; RRID: AB_10127658 Phospho-Ubiquitin (Ser65) (E2J6T) Rabbit mAb Cell Signaling Technology Cat#62802; RRID: AB_2799632 COX IV (4D11-B3-E8) Mouse mAb Cell Signaling Technology Cat#11967; RRID: AB_2797784 SLP-2 (D2I9X) Rabbit Cell Signaling Technology Cat#89199; RRID: AB_2800134 PKA RI-alpha/beta Antibody Cell Signaling Technology Cat#3927; RRID: AB_1658217 PKA C-alpha (D38C6) Rabbit mAb Cell Signaling Technology Cat#5842; RRID: AB_10706172 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb Cell Signaling Technology Cat#9624; RRID: AB_331817 Anti-FLAG M2 Mouse mAb Millipore Sigma Cat#F3165; RRID: AB_259529 Vinculin (E1E9V) Rabbit mAb Cell Signaling Technology Cat#13901; RRID:AB_2728768 Goat anti-mouse IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#1030–05; RRID:AB_2619742 Goat anti-rabbit IgG (H + L) Secondary Antibody, HRP Southern Biotech Cat#4030–05; RRID:AB_2687483 Bacterial and virus strains Herpes Simplex Virus 1 (HSV-1)-GFP Vector Builder Cat#: HSVSP(HSV001) Chikungunya virus (CHIKV) strain 181/25 ATCC Cat#: VR-3360 Chemicals, peptides, and recombinant proteins 16,16-dimethyl Prostaglandin E2 Cayman Chemical Cat#:14750 Forskolin Millipore Sigma Cat#:F3917 BLU0588 TargetMol Cat#:T60169 ONO AE3 208 Tocris Bioscience Cat#:3565 IBMX Millipore Sigma Cat#:I5879 Quant-iTTM PicoGreenTM dsDNA Reagent Invitrogen Cat#:P11495 MitoTracker Deep Red FM Thermo Fisher Cat#:M22426 MitoTracker Green FM Thermo Fisher Cat#:M7514 NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33342) Invitrogen Cat#:R37605 2′,3′-Dideoxycytidine Millipore Sigma Cat#:D5782 Celecoxib Selleckchem Cat#:S1261 Cycloheximide Millipore Sigma Cat#:C4859 DYKDDDDK Fab-Trap Agarose Proteintech Cat#:ffa; RRID:AB_2894836 (Continued on next page) 20 Cell Reports 45, 118154, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER RIPA Lysis and Extraction Buffer Thermo Scientific Cat#: 89900 Pierce IP Lysis Buffer Thermo Scientific Cat#:87788 HaltTM Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Scientific Cat#:78446 Sodium Orthovanadate (Vanadate) New England Biolabs Cat#:P0758 4x Laemmli Sample Buffer Biorad Cat#:1610747 DMEM Millipore Sigma Cat#:D6429 RPMI 1640 Medium (ATCC modification) Gibco Cat#:A1049101 FluoroBrite DMEM Gibco Cat#:A1896701 Fetal Bovine Serum (FBS) Millipore Sigma Cat#:12103C Antibiotic-Antimycotic Gibco Cat#:15240062 2-mercaptoethanol Gibco Cat#:21985023 Phorbol 12-myristate 13-acetate Millipore Sigma Cat#:P8139 Advanced Dulbecco’s Modified Eagle’s Medium/F12 medium Gibco Cat#:12634010 Mouse M-CSF Recombinant Protein PeproTech Cat#:315–02 Human M-CSF Recombinant Protein PeproTech Cat#:300–25–1 Trypsin-EDTA Gibco Cat#:25300054 Pierce ECL Western Blotting Substrate Thermo Scientific Cat#:32106X4 Immobilon ECL UltraPlus Western HRP Substrate Millipore Sigma Cat#:WBULP Rp-8-AHA-cAMPS-Agarose Biolog Cat#:A012 8-AHA-cAMPS-Agarose Biolog Cat#:A028 HaloLink-Agarose Promega Cat#:G1914 HSV-60 - CDS Agonist InvivoGen Cat#: tlrl-hsv60n EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#:AM9260G HEPES (1M) Gibco Cat#:15630080 Polybrene Millipore Sigma Cat#:107689 LR Clonase Invitrogen Cat#:11791100 Lipofectamine RNAiMAX Invitrogen Cat#:13778150 Puromycin Gibco Cat#:A1113803 Turbofect Transfection Reagent Thermo Scientific Cat#:R0531 Lenti-X concentrator Takara Cat#:631232 Immobilon PVDF Membrane Millipore Sigma Cat#:IPVH304F0 DNeasy Blood & Tissue Kit Qiagen Cat#:69504 Benzonase Millipore Sigma Cat#:E1014-25KU Complete Protease Inhibitor Cocktail Millipore Sigma Cat#:11697498001 FLAG peptide Millipore Sigma Cat#:F3290-4MG RapiGest Waters Corporation Cat#:186001861 Trypsin Promega Cat#:V5113 C18 tips NEST Group Cat#:HNS S18V DPBS, no calcium, no magnesium Gibco Cat#:14190136 Critical commercial assays RNeasy Mini Kit (50) Qiagen Cat#:74104; SuperScriptTM VILOTM Master Mix Invitrogen Cat#11755050; Fast SYBR Green Master Mix Applied Biosystems Cat#:4385612; (Continued on next page) Cell Reports 45, 118154, October 27, 2026 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prostaglandin E2 ELISA Kit - Monoclonal Cayman Chemical Cat#514010; QIAquick Nucleotide Removal Kit Qiagen Cat#28306; Mitochondria Isolation Kit for Cultured Cells Thermo Scientific Cat#89874; Human IFN-Beta TCM ELISA, High Sensitivity PBL Assay Science Cat#41435; SYBRTM Green Universal Master Mix Applied Biosystems Cat#4309155; 2′3′-cGAMP ELISA Kit Cayman Cat#501700; cAMP ELISA kit Enzo Cat#:ADI-900-067A; Dual-Glo Luciferase Assay System Promega Cat#E2920; Deposited data Original 4D imaging data analysis This paper Zenodo: https://doi.org/10.5281/ zenodo.22713939 Raw bulk RNA-Seq data This paper GEO: GSE324662 Raw MS data files, search results and individual spectral libraries This paper ProteomeXchange Consortium: PXD075490 Experimental models: Cell lines THP-1 ATCC Cat#:TIB-202; RRID:CVCL_0006 THP-1 ISRE-Luc2 ATCC Cat#:TIB-202-ISRE-LUC2; RRID:CVCL_A4CC HEK293 ATCC Cat#:CRL-1573; RRID:CVCL_0045 BHK-21 [C-13] ATCC Cat#:CCL-10; RRID:CVCL_1915 Primary Peripheral Blood Mononuclear Cells (PBMC), Normal, Human ATCC Cat#:PCS-800–010; THP-1 mt-mKeima This paper NA THP-1 STOML2 WT This paper NA THP-1 STOML2 S29A This paper NA THP-1 STOML2 S29D This paper NA Experimental models: Organisms/strains Mouse: C57BL/6 The Jackson Laboratory CAT#:000664; RRID:IMSR_JAX:000664 Oligonucleotides All oligonucleotides used in this study are listed in Table S1 This paper Table S1 Recombinant DNA pCMV-STOML2-WT-3xFLAG This paper NA pCMV-STOML2-S29A-3xFLAG This paper NA pCMV-STOML2 WT-3xFLAG This paper NA pLenti-STOML2-WT-3xFLAG This paper NA pLenti-STOML2-S29A-3xFLAG This paper NA pLenti-STOML2-S29D-3xFLAG This paper NA pCHAC-mt-mKeima Addgene Cat#:72342; RRID:Addgene_72342 pLenti-mt-mKeima This paper NA pLVX-TetOn-PRKACA-WT-3XFLAG This paper NA pLVX-TetOn-PRKACA-W197R3XFLAG This paper NA (Continued on next page) 22 Cell Reports 45, 118154, October 27, 2026
Cell Culture:
Article Title: Enzyme‐Linked Cycloaddition Assay (ELCA) for Rapid, Ultra‐Sensitive Monitoring of Secreted Sialoglycoproteins
Article Snippet: A375 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) with high glucose, 4.0 m m L‐glutamine, and sodium pyruvate (SH30243.01, Cytiva). .. THP‐1 cells (TIB‐202, ATCC) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (ATCC modification; A1049101, Gibco). .. The respective growth media were supplemented with 10% (v/v) heat‐inactivated fetal bovine serum (FBS; FBS‐HI‐12A, Capricorn Scientific) and 1% (v/v) penicillin‐streptomycin solution (P4333‐100ML, Sigma‐Aldrich).
Article Title: PLK1 inhibition by onvansertib enhances paclitaxel efficacy and overcomes resistance in HR+/HER2- breast cancer refractory to endocrine therapy and CDK4/6 inhibitors
Article Snippet: .. MCF7 cells were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; 118 ATCC, Cat. No. 30-2002) supplemented with 10% fetal bovine serum (FBS; ATCC, Cat. No. 30-119 2020), while all other parental cell lines were maintained in RPMI-1640 medium (ATCC, Cat. No. 120 30-2001) with 10% FBS. .. The fulvestrant-resistant MCF7 cell line, MCF7/164R-7 (MCF7/FR; 121 CancerTools, Cat. No. 152103; RRID: CVCL_1D39), was cultured in phenol red–free DMEM/F12 122 (Gibco, Cat. No. 11039021) supplemented with 1% FBS and 100 nM fulvestrant (Tocris, Cat. No. 123 1047).
Cell Adhesion Assay:Article Title: OGT-mediated O-GlcNAcylation of TEAD1 at Serine 132 Enhances TEAD1 Stability and Activity and Contributes to Atherosclerosis Progression
Article Snippet: .. 389 2.15 Monocyte Adhesion Assay 390 THP-1 human monocytic cells (ATCC) were maintained in RPMI-1640 medium 391 supplemented with 10% FBS and 1% penicillin/streptomycin. .. For adhesion 392 assays, THP-1 cells were labeled with 5 μM Calcein-AM (Invitrogen) for 30 393 minutes at 37°C, washed twice with serum-free medium, and resuspended in 394 RPMI-1640 at a density of 1 × 10^6 cells/mL.
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