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    Qiagen stop solution
    Stop Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stop+solution/QIAzol+Lysis+Reagent/pmc13062092-270-6-14
    Average 99 stars, based on 9945 article reviews
    stop solution - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Tissue:

    Article Title: Hesperidin Attenuates Vincristine‐Induced Nephrotoxicity via Nrf2/HO‐1 and PI3K/Akt/mTOR Signaling
    Article Snippet: .. Total RNA was extracted from tissue samples using QIAzol Lysis Reagent (Qiagen, Germany; Cat. No. 79306). .. The RNA concentrations were standardized across samples, and the RNA purity and quantity were assessed using a NanoDrop spectrophotometer (BioTek Instruments, EPOCH, USA).

    Article Title: Cardioprotective Effect of Metformin Treatment in Mouse Model of Experimental Autoimmune Myocarditis
    Article Snippet: All stained sections were analyzed using ImageJ software (Version 1.52a, NIH, United States) and ImageScope software (Version 12.3.3, Leica Biosystems Imaging, Germany). .. Total RNA was isolated from mouse heart tissue using QIAzol Lysis Reagent (Qiagen, Germany), following the manufacturer′s protocol. .. Briefly, samples were lysed directly in QIAzol and homogenized using a TissueLyser II system (Qiagen, Germany).

    Article Title: Smooth muscle cell mineralocorticoid receptor does not contribute to atherosclerosis in male or female ApoE-knockout mice
    Article Snippet: .. Frozen tissue was homogenized in QIAzol lysis reagent (QIAGEN). .. After chloroform phase-separation, the aqueous phase was transferred to a fresh tube, mixed with 100% ethanol, and total RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions.

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Lysis:

    Article Title: Hesperidin Attenuates Vincristine‐Induced Nephrotoxicity via Nrf2/HO‐1 and PI3K/Akt/mTOR Signaling
    Article Snippet: .. Total RNA was extracted from tissue samples using QIAzol Lysis Reagent (Qiagen, Germany; Cat. No. 79306). .. The RNA concentrations were standardized across samples, and the RNA purity and quantity were assessed using a NanoDrop spectrophotometer (BioTek Instruments, EPOCH, USA).

    Article Title: Cardioprotective Effect of Metformin Treatment in Mouse Model of Experimental Autoimmune Myocarditis
    Article Snippet: All stained sections were analyzed using ImageJ software (Version 1.52a, NIH, United States) and ImageScope software (Version 12.3.3, Leica Biosystems Imaging, Germany). .. Total RNA was isolated from mouse heart tissue using QIAzol Lysis Reagent (Qiagen, Germany), following the manufacturer′s protocol. .. Briefly, samples were lysed directly in QIAzol and homogenized using a TissueLyser II system (Qiagen, Germany).

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Article Title: Pharmacologically Targeting DHODH-Dependent Pyrimidine Metabolism with HSA-Conjugated BRQ Suppresses Leukemogenesis.
    Article Snippet: .. Total RNA was extracted using QIAzol Lysis Reagent (79306, QIAGEN, Germantown, MD, USA) according to the manufacturer’s instructions. .. Reverse transcription was performed with 500–1000 ng of total RNA using the QuantiTect Reverse Transcription kit (205314, QIAGEN) according to the manufacturer’s instructions.

    Article Title: Smooth muscle cell mineralocorticoid receptor does not contribute to atherosclerosis in male or female ApoE-knockout mice
    Article Snippet: .. Frozen tissue was homogenized in QIAzol lysis reagent (QIAGEN). .. After chloroform phase-separation, the aqueous phase was transferred to a fresh tube, mixed with 100% ethanol, and total RNA was purified using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions.

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Article Title: Chrysin as a multi-target regulator of tramadol-induced testicular damage: Integration of inflammation, mitochondrial dynamics, and germ cell survival pathways.
    Article Snippet: Tramadol, although commonly utilized as an analgesic, is an opioid derivative that may induce significant adverse consequences on the reproductive system.. This study experimentally assessed the toxic effects of tramadol on testicular tissue and reproductive capabilities, as well as the protective capability of chrysin (CHR) against these effects.. The results indicated that tramadol treatment caused considerable functional impairment in testicular tissue, as reflected by a notable reduction in sperm count, motility, and morphology, an elevation in the Cosentino score, and a decline in the Johnsen score and germinal epithelial thickness.

    Article Title: microRNA and Regulatory Networks of Human Macrophage During Leishmania major In Vitro Infection.
    Article Snippet: To enable the simultaneous profiling of mRNA and miR from each single sample, total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Hilden, Germany). .. Cells were harvested and immediately lysed with 700 μL of QIAzol Lysis Reagent (Qiagen, Hilden, Germany), and the RNA extraction proceeded following the manufacturer’s instructions. .. RNA concentration and quality were determined using a NanoDrop microspectrophotometer and a Qubit fluorometer 3 (Thermofisher, Carlsbad, CA, USA).

    Isolation:

    Article Title: Cardioprotective Effect of Metformin Treatment in Mouse Model of Experimental Autoimmune Myocarditis
    Article Snippet: All stained sections were analyzed using ImageJ software (Version 1.52a, NIH, United States) and ImageScope software (Version 12.3.3, Leica Biosystems Imaging, Germany). .. Total RNA was isolated from mouse heart tissue using QIAzol Lysis Reagent (Qiagen, Germany), following the manufacturer′s protocol. .. Briefly, samples were lysed directly in QIAzol and homogenized using a TissueLyser II system (Qiagen, Germany).

    Cell Culture:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Crocin Bleaching Assay:

    Article Title: A natural compound derivative targeting HADHA disrupts mitochondrial homeostasis and suppresses renal cell carcinoma.
    Article Snippet: Ara Jo, Sanjeevram Dhandapani, Ziqin Wang, Hyeryeon Jung, Hongjun Jang, Morgan A. Bailey, Andrew Tran, Brandon Tsai, Jinjae Park, Hyejin Lee, Hye-Jin Park, Yoojin Lim, Dolce Feenaghty, Victor M. Moncada, Damu Wu, Keaton Chien, Nancy Chen, Yunsol Jo, Jiazheng Miao, Zhujun Yao, Jihoon Lee, Karthigayan Shanmugasundaram, Gang Greg Wang, Andrew Armstrong, Daniel George, David Howell, Jiaoti Huang, Michael C. Fitzgerald, Jiyong Hong, Jung Wook Park

    Protease Inhibitor:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Glass:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Saline:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Transcranial Magnetic Stimulation:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Mass Spectrometry:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    cDNA Synthesis:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    BIA-KA:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Purification:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    CCK-8 Assay:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Titration:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    Sequencing:

    Article Title: A systemic circadian nicotinic acid riboside (NaR) signal engages the unfolded protein response and adipogenesis via the prefoldin complex.
    Article Snippet: REAGENT or RESOURCES SOURCE IDENTIFIER Antibodies anti-CEBPA Cell Signaling Cat#8178; RRID: AB_11178517 anti-ATF4 Cell Signaling Cat#11815; RRID: AB_2616025 anti-XBP1s Cell Signaling Cat#40435; RRID: AB_2891025 anti-ATF6 Novus Biologicals Cat#40256; RRID: AB_2058774 anti-alpha-TUBULIN Cell Signaling Cat#9099; RRID: AB_10695471 anti-P84 Genetex Cat#GTX70220; RRID: AB_372637 Rabbit IgG-HRP Sigma-Aldrich Cat#A9169; RRID: AB_258434 Mouse IgG-HRP Sigma-Aldrich Cat#5278; RRID: AB_258232 Biological samples Mouse skeletal muscle, C57BL/6 This study N/A Mouse epididymal white adipose tissue (eWAT), C57BL/6 This study N/A Mouse brain tissue, C57BL/6 This study N/A Chemicals, peptides, and recombinant proteins DharmaFECT 1 Transfection Reagent Horizon Discovery Cat#T-2001-02 DMEM 1.0g/L Glucose w/o L-Gln Lonza Cat#12-707F SYBR-green Master Mix Bio-Rad Cat#1708884 Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat#RPN2232 Blotto Immunoanalytical Grade (Non-Fat Dry Milk) BioNordika Cat#B501-0500 DTT Sigma-Aldrich Cat#10197777001 2-Mercaptoethanol Sigma-Aldrich Cat#M3148-100ML RIPA buffer Thermo Fisher Scientific Cat#89901 FGF2 human Sigma-Aldrich Cat#F0291 Insulin (liquid, ready to use, 10mg/mL, 5mL) Sigma-Aldrich Cat#I9278 T3 Sigma-Aldrich Cat#T6397 Transferrin Sigma-Aldrich Cat#T8158 IBMX (3-Isobutyl-1-methylxanthin) - for human cell line Sigma-Aldrich Cat#I5879 Rosiglitazone (100 mg) Cayman Chemicals Cat#71740 Dexamethasone Sigma-Aldrich Cat#D1756 IBMX (3-Isobutyl-1-methylxanthin) - for murine cell lines Sigma-Aldrich Cat#7018 Bovine insulin Sigma-Aldrich Cat#I6634 0.5% Trypsin/EDTA (10x) Invitrogen (GIBCO) Cat#15400-054 Penicilline G 10000U/mL/Streptomycine 10000μg/mL Invitrogen (GIBCO) Cat#15140-122 HEPES 1M Invitrogen (GIBCO) Cat#15630-056 Ham’s F-12 Nutrient Mix Invitrogen (GIBCO) Cat#21765-037 DMEM, low glucose, pyruvate (+glutamine) Invitrogen (GIBCO) Cat#31885-023 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 Bodipy 493/503 Thermo Fisher Scientific Cat#D3922 Hoechst Thermo Fisher Scientific Cat#34580 (Continued on next page) 18 Cell Reports 45, 118136, October 27, 2026 .. REAGENT or RESOURCES SOURCE IDENTIFIER Precision Plus Protein Blue-Stained Protein Standards 10-250kDa Bio-Rad Cat#1610373 Complete, EDTA-free Protease Inhibitor Cocktail, Tablets in glass vials Sigma-Aldrich Cat#5056489001 PhosSTOP Sigma-Aldrich Cat#4906837001 QIAzol lysis reagent Qiagen Cat79306 DMEM/F12, HEPES Invitrogen (GIBCO) Cat#11330032 Donor Bovine Serum, New Zealand Origin Invitrogen (GIBCO) Cat#16030074 Fetal Bovine Serum Invitrogen (GIBCO) Cat#A5256701 1X phosphate-buffered saline, pH 7.4 Invitrogen (GIBCO) Cat#10010023 Antibiotic-Antimycotic (100X) Invitrogen (GIBCO) Cat#15240062 DMEM - high glucose Sigma-Aldrich Cat#D6429 L-Glutamine Solution 200 mM Sigma-Aldrich Cat#59202C Nicotinic acid riboside - for cells Toronto Research Chemicals Inc. Cat#TRC-N429400 Nicotinic acid riboside - for oral gavage AmBeed Cat#17720-18-2 β- nicotinamide mononucleotide (NMN) Sigma-Aldrich Cat #N3501 Sodium chloride solution Sigma-Aldrich Cat #S8776 4μ8C Sigma-Aldrich Cat#SML0949 Ceapin-A7 Sigma-Aldrich Cat#SML2330 GSK2656157 Sigma-Aldrich Cat#17372 Tunicamycin from Streptomyces sp. Sigma-Aldrich Cat#T7765 TBS with TweenTM (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Thermo Fisher Scientific Cat#J77500.K2 Iodoacetamide Sigma-Aldrich Cat#I1149 Lysyl Endopeptidase MS Wako Chemicals Cat#125-05061 Acetonitrile Sigma-Aldrich Cat#1000291000 Hydroxylamine Sigma-Aldrich Cat#159417 NP-40 Thermo Fisher Scientific Cat#85124 Insulin-Transferrin-Selenium (ITS -G) (100X) Thermo Fisher Scientific Cat#41400045 60% high fat diet Ssniff Spezialdiäten Gmbh Cat#D12492 Critical commercial assays iScript cDNA Synthesis kit Bio-Rad Cat#170-8891 Pierce BCA Protein Assay kit Thermo Fisher Scientific Cat#23227 NucleoSpin RNA, Mini kit for RNA purification Macherey-Nagel Cat#32–740955.250 Cell Counting Kit-8 MedChemExpress Cat#HY-K0301 RNeasy Lipid Tissue Mini Kit Qiagen Cat#74804 Stainless Steel Beads, 5 mm (200) Qiagen Cat#69989 Deposited data NaR titration sequencing This study GSE320452 Conditioned media sequencing This study GSE333546 Adipocyte proteomics raw and processed data This study ProteomeXchange: PXD074620; MassIVE: MSV000100889 Experimental models: Cell lines 3T3-L1 Provided by Anna Krook’s lab N/A HT-22 Milipore Sigma Cat#SCC129 C2C12 Provided by Anna Krook’s lab N/A Human preadipocytes Provided by Ryden/ Mejhert’s lab N/A AML12 in house N/A (Continued on next page) Cell Reports 45, 118136, October 27, 2026 19 ..

    RNA Extraction:

    Article Title: microRNA and Regulatory Networks of Human Macrophage During Leishmania major In Vitro Infection.
    Article Snippet: To enable the simultaneous profiling of mRNA and miR from each single sample, total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Hilden, Germany). .. Cells were harvested and immediately lysed with 700 μL of QIAzol Lysis Reagent (Qiagen, Hilden, Germany), and the RNA extraction proceeded following the manufacturer’s instructions. .. RNA concentration and quality were determined using a NanoDrop microspectrophotometer and a Qubit fluorometer 3 (Thermofisher, Carlsbad, CA, USA).



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