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ip1 stimulation buffer  (Cisbio Bioassays)

 
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    Cisbio Bioassays ip1 stimulation buffer
    Ip1 Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stimulation+buffer/ip1+stimulation+buf/us12012419-481-14-17
    Average 90 stars, based on 1 article reviews
    ip1 stimulation buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Stable Transfection:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Expressing:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Cell Culture:

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Article Title: Heterocyclic compound
    Article Snippet: To a 384-well white plate (Greiner) was added 4 μL of the test compound diluted with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA containing 80 nM acetylcholine. .. Freeze stock of CHO-K1 cells stably expressing human M1 receptor (hCHRM1) was prepared with IP1 Stimulation buffer (CisBio) containing 0.1% fatty acid-free BSA, 4 μL thereof was added (10,000 cells/well) and the mixture was cultured in a 5% CO2 incubator at 37° C. for 1 hr. .. 4 μL of a solution (CisBio) containing IP1-d2 and Anti-IP1-cryptate Tb conjugate was added, the mixture was incubated at room temperature for 1 hr, and the time-resolved fluorescence signal was measured on an Envision plate reader (PerkinElmer).

    Transfection:

    Article Title: The intramolecular agonist is obligate for activation of glycoprotein hormone receptors.
    Article Snippet: To perform the assay, Cisbio's IP-One Tb kit (Cisbio, Codolet, France) was used. .. Forty-eight hours after transfection, cells were stimulated for 1 hour at 37°C with 35 μL 1 × IP1 stimulation buffer (Cisbio) containing the respective reagents. .. Cells were lysed by adding 30 μL lysis buffer (Cisbio) per well and kept frozen at −20°C until measurement.

    Article Title: Mapping the Binding Sites of UDP and Prostaglandin E2 Glyceryl Ester in the Nucleotide Receptor P2Y 6
    Article Snippet: .. Then, 48 h after transfection, cells were stimulated 30 min at 37 °C with 35 μl 1× IP 1 stimulation buffer (Cisbio) containing the respective reagents (concentrations as indicated). .. Next, cells were lysed by adding 30 μl lysis buffer (Cisbio) per well and kept frozen at −20 °C until measurement.



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    Image Search Results


    (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Journal: PLOS Biology

    Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode

    doi: 10.1371/journal.pbio.3002838

    Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

    Article Snippet: The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C.

    Techniques: Inhibition, Transfection, Control, Expressing