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stemfit basic02 medium  (Ajinomoto Althea)


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    Ajinomoto Althea stemfit basic02 medium
    Stemfit Basic02 Medium, supplied by Ajinomoto Althea, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemfit+basic02+medium/StemFit%C3%82+Basic02+-+Stem+Cell+Culture+Media/us12351605-474-16-19
    Average 94 stars, based on 50 article reviews
    stemfit basic02 medium - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Enhanced survival of hypoimmunogenic otic progenitors following intracochlear xenotransplantation – Repercussions for stem cell therapy in hearing loss models
    Article Snippet: .. First, undifferentiatied iPSCs were cultured in StemFit Basic02 media (Ajinomoto CO., INC, Tokyo, Japan) on iMatrix-511TM (Nippi, Tokyo, Japan). .. After magnetic purification of undifferentiated iPSCs using an anti-TRA-1-60 microbeads kit, LS Columns, and a MidiMACS Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), cells were seeded into a chemically defined medium containing: 40% DMEM/F12 medium (Gibco, Gaithersburg, MD, USA), 40% Neurobasal medium (Gibco) and 20% StemFit for Differentiation supplement (Ajinomoto).

    Article Title: Modelling co-development between the somites and neural tube in human Trunk-like Structures (hTLS)
    Article Snippet: .. Human iPS cells were cultured routinely in either StemFit Basic 02 (Ajinomoto, discontinued in EU) supplemented with 80 ng/mL bFGF (Peprotech, 100-18B) or mTeSR TM 1 (STEMCELL Technologies, 85850) on 0.25 μg/cm 2 recombinant Laminin iMatrix-511 silk E8- coated plates (AMSbio, AMS892 021). .. Cultures of 80-90% confluency were routinely passaged using Accutase TM (STEMCELL Technologies, 07922) and single cells were seeded with medium supplemented with 10 μM Y- 27632 (ROCK inhibitor; Tocris Biosciences, 1254).

    Article Title: Optically controllable FGFR stimulation using wireless controlled cellular lighting system
    Article Snippet: Ten ng/mL of FGF2 (R&D Systems) was added after sterile filtration, and the cells were fed daily and passaged weekly using either 6 U/mL Dispase (Life Technologies) or mechanical means. .. For feeder-free culture conditions, hPSCs were cultured onto recombinant human Laminin 521 (Life Technologies)-coated plates with StemFit® Basic02 medium (Ajinomoto). .. The porcine induced pluripotent stem cells (induced from P350-05; PCASMC, porcine coronary artery smooth muscle cells from Sigma-Aldrich) were cultured with mitotically inactivated MEFs in the PSC medium, and the cells were fed daily and passaged weekly using either 6 U/mL Dispase or mechanical means.

    other:

    Article Title: Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial integration
    Article Snippet: 6 human iPSCs (passage 30–50) were maintained in either StemFit® Basic02 (Ajinomoto, Japan) supplemented with FGF2 (10 ng/ml) (Peprotech, #100-18B) or StemFit® Basic04 Complete type (Ajinomoto, Japan) in 6-well tissue culture plates (Falcon, #353046) coated with 1% vol/ LDEV-Free hESC-qualified Geltrex (Life Technologies, #A1413302) in a 37°C incubator with 5% CO2.

    Article Title: Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial integration.
    Article Snippet: 6 human iPSCs (passage 30–50) were maintained in either StemFit® Basic02 (Ajinomoto, Japan) supplemented with FGF2 (10 ng/ml) (Peprotech, #100-18B) or StemFit® Basic04 Complete type (Ajinomoto, Japan) in 6-well tissue culture plates (Falcon, #353046) coated with 1% vol/ LDEV-Free hESC-qualified Geltrex (Life Technologies, #A1413302) in a 37°C incubator with 5%CO2.

    Recombinant:

    Article Title: Modelling co-development between the somites and neural tube in human Trunk-like Structures (hTLS)
    Article Snippet: .. Human iPS cells were cultured routinely in either StemFit Basic 02 (Ajinomoto, discontinued in EU) supplemented with 80 ng/mL bFGF (Peprotech, 100-18B) or mTeSR TM 1 (STEMCELL Technologies, 85850) on 0.25 μg/cm 2 recombinant Laminin iMatrix-511 silk E8- coated plates (AMSbio, AMS892 021). .. Cultures of 80-90% confluency were routinely passaged using Accutase TM (STEMCELL Technologies, 07922) and single cells were seeded with medium supplemented with 10 μM Y- 27632 (ROCK inhibitor; Tocris Biosciences, 1254).

    Article Title: Optically controllable FGFR stimulation using wireless controlled cellular lighting system
    Article Snippet: Ten ng/mL of FGF2 (R&D Systems) was added after sterile filtration, and the cells were fed daily and passaged weekly using either 6 U/mL Dispase (Life Technologies) or mechanical means. .. For feeder-free culture conditions, hPSCs were cultured onto recombinant human Laminin 521 (Life Technologies)-coated plates with StemFit® Basic02 medium (Ajinomoto). .. The porcine induced pluripotent stem cells (induced from P350-05; PCASMC, porcine coronary artery smooth muscle cells from Sigma-Aldrich) were cultured with mitotically inactivated MEFs in the PSC medium, and the cells were fed daily and passaged weekly using either 6 U/mL Dispase or mechanical means.



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