stemdiff cardiomyocyte differentiation kit (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc stemdiff cardiomyocyte differentiation kit

Activation and Killing by T Cells Is Selective for iPSC-β (A) Experimental design: PBMCs co-cultured with autologous iPSC-derived cells. (B, C, and F) CD25 or CD69 expression shown as MFI. (B) PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous iPSC-β (purple) or iPSC-α (red). (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (C) Donor-matched PBMCs (CD3 + gated) (n = 1 T1D, n = 3 differentiation batches per donor line) co-cultured for 48 h with autologous iPSC-β (purple) or <t>iPSC-cardiomyocytes</t> (orange). (D) Percentage of live iPSC-β (C-peptide + /glucagon − ) or iPSC-α (C-peptide − /glucagon + ) from iPSC-β or iPSC-α differentiations, respectively (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (F) Representative flow cytometry histograms after 48 h of co-culture. Dashed histogram represents the control (untreated target cells). (E) Percentage of apoptotic (apopxin + ) iPSC-β (CD49a + /CD26 − ) or iPSC-α (CD49a − /CD26 + ) from iPSC-β or iPSC-α differentiations (n = 1 T1D iPSC donor, n = 3 differentiation batches per donor line). (G) Unmatched PBMCs (CD3 + gated cells) co-cultured for 48 h with iPSC-α (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (H) Donor-matched PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous enriched iPSC-β or iPSC-α. Data are means ± SEMs, 2-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, and ∗∗∗∗ p < 0.0001; ns, non-significant. See also <xref ref-type=

Figure S4 . " width="250" height="auto" />
Stemdiff Cardiomyocyte Differentiation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemdiff+co+kit/stemdiff+cardiomyocyte+differentiation+kit/pmc07359783-67-0-5
Average 90 stars, based on 1 article reviews

stemdiff cardiomyocyte differentiation kit - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells"

Article Title: Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells

Journal: Cell Reports

doi: 10.1016/j.celrep.2020.107894

Figure S4 . " title="... for 48 h with autologous iPSC-β (purple) or iPSC-cardiomyocytes (orange). (D) Percentage of live iPSC-β (C-peptide + ..." property="contentUrl" width="100%" height="100%"/>
Figure Legend Snippet: Activation and Killing by T Cells Is Selective for iPSC-β (A) Experimental design: PBMCs co-cultured with autologous iPSC-derived cells. (B, C, and F) CD25 or CD69 expression shown as MFI. (B) PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous iPSC-β (purple) or iPSC-α (red). (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (C) Donor-matched PBMCs (CD3 + gated) (n = 1 T1D, n = 3 differentiation batches per donor line) co-cultured for 48 h with autologous iPSC-β (purple) or iPSC-cardiomyocytes (orange). (D) Percentage of live iPSC-β (C-peptide + /glucagon − ) or iPSC-α (C-peptide − /glucagon + ) from iPSC-β or iPSC-α differentiations, respectively (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (F) Representative flow cytometry histograms after 48 h of co-culture. Dashed histogram represents the control (untreated target cells). (E) Percentage of apoptotic (apopxin + ) iPSC-β (CD49a + /CD26 − ) or iPSC-α (CD49a − /CD26 + ) from iPSC-β or iPSC-α differentiations (n = 1 T1D iPSC donor, n = 3 differentiation batches per donor line). (G) Unmatched PBMCs (CD3 + gated cells) co-cultured for 48 h with iPSC-α (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (H) Donor-matched PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous enriched iPSC-β or iPSC-α. Data are means ± SEMs, 2-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, and ∗∗∗∗ p < 0.0001; ns, non-significant. See also Figure S4 .

Techniques Used: Activation Assay, Cell Culture, Derivative Assay, Expressing, Flow Cytometry, Co-Culture Assay, Control

Figure Legend Snippet:

Techniques Used: Virus, Recombinant, Software, Real-time Polymerase Chain Reaction

Transfection:

Article Title: AEOL-induced NRF2 activation and DWORF overexpression mitigate myocardial I/R injury.
Article Snippet: hiPSCMs were transfected with specific siRNAs targeting NRF2 (Accell Human NFE2L2 siRNA, DHARMACON) or DWORF (SIRGT66230 WQ-2OMe, CREATIVE BIOLABS) using LipofectamineTM MessengerMAXTM Transfection reagent (Thermo Fisher). .. Cells were seeded 2 days before transfection in 6-well plates with 2 mL STEMdiff Cardiomyocyte Support Medium (STEMCELL Technologies) at 60% confluence. ..

Centrifugation:

Article Title: Propionic Acidemia-Induced Proarrhythmic Electrophysiological Alterations in Human iPSC-Derived Cardiomyocytes.
Article Snippet: Magnetic separation was subsequently performed according to the manufacturer's instructions using LS columns and pre- separation filters (70 μm) (both from Miltenyi Biotec), and an appropriate MACS separator. .. After a final centrifugation at 200×g for 5 min, the enriched cardiomyocytes were resuspended in STEMdiff Cardiomyocyte Support Medium (StemCell Technologies) containing 10 μM Rock inhibitor and seeded onto Matrigel (Corning Matrigel Basement Membrane Matrix)- coated 24- well plates with a cover slip (12 mm diameter, Epredia, DA Breda, Netherlands). .. The culture medium was replaced with STEMdiff Cardiomyocyte Maintenance Kit (StemCell Technologies) the next day, and the medium was subsequently changed every two days over a period of 7–9 days.

Article Title: Propionic Acidemia‐Induced Proarrhythmic Electrophysiological Alterations in Human iPSC ‐Derived Cardiomyocytes
Article Snippet: Magnetic separation was subsequently performed according to the manufacturer's instructions using LS columns and pre‐separation filters (70 μm) (both from Miltenyi Biotec), and an appropriate MACS separator. .. After a final centrifugation at 200×g for 5 min, the enriched cardiomyocytes were resuspended in STEMdiff Cardiomyocyte Support Medium (StemCell Technologies) containing 10 μM Rock inhibitor and seeded onto Matrigel (Corning Matrigel Basement Membrane Matrix)‐coated 24‐well plates with a cover slip (12 mm diameter, Epredia, DA Breda, Netherlands). .. The culture medium was replaced with STEMdiff Cardiomyocyte Maintenance Kit (StemCell Technologies) the next day, and the medium was subsequently changed every two days over a period of 7–9 days.

Membrane:

other:

Article Title: In vivo assessment of iPSC-cardiomyocyte loaded auxetic cardiac patches following chronic myocardial infarction.
Article Snippet: Novel cardiac patch designs achieved by advanced 3D manufacturing continue to have favorable impacts on the repair and regeneration of the myocardium after injury.. Briefly, auxetic units with a negative Poisson’s ratio have already shown remarkable promise for serving as a next-generation complex scaffold in left ventricular disease.. In this study we biofabricated a 3D printed polycaprolactone (PCL) cardiac auxetic patch loaded with high density contractile induced pluripotent stem cell-derived cardiomyocytes (iCMs) and examined the synergist effect of iCM auxetic patches on a chronic myocardial infarct rodent model compared to a stiffer non-auxetic control patch architecture.

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