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stem loop primers  (Thermo Fisher)


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    Thermo Fisher stem loop primers
    Stem Loop Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem+loop+primers/SUCROSE+EP%2FBP%2FNF+12KG/pm41816892-204-2-20
    Average 99 stars, based on 1 article reviews
    stem loop primers - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Bacteria:

    Article Title: Anti-αβTCR antibody
    Article Snippet: USA, 77(7):4216-4220), 293 (ATCC Accession No. CRL-1573), HeLa (ATCC Accession No. CCL-2), CVI (ATCC Accession No. CCL-70), WOP (Dailey, L., et al. (1985) J. .. PerC6 (Crucell, Leiden, NL), B. subtilis and/or other suitable bacteria; eukaryotic cells, such as fungal or yeast cells (e.g., Pichia pastoris, Aspergillus sp., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Neurospora crassa), or other lower eukaryotic cells, and cells of higher eukaryotes such as those from insects (e.g., Drosophila Schnieder S2 cells, Sf9 insect cells) (WO 94/126087 (O'Connor)), BTI-TN-5B1-4 (High FiveTM) insect cells (Invitrogen), mammals (e.g., COS cells, such as COS-1 (ATCC Accession No. CRL-1650) and COS-7 (ATCC Accession No. CRL-1651), CHO (e.g., ATCC Accession No. CRL-9096), CHO DG44 (Urlaub, G. and Chasin, L. A. (1980) Proc. ..

    Amplification:

    Article Title: Re-engineering segment 8 facilitates generation of a versatile live-attenuated influenza A virus vector platform for secretory protein delivery.
    Article Snippet: .. Segment 8 was amplified from the viral RNAs via segment-specific RT-PCR using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) and primers 4F and 4R (see Table S2). .. Amplicons were confirmed by Sanger sequencing (Eton Bioscience).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Re-engineering segment 8 facilitates generation of a versatile live-attenuated influenza A virus vector platform for secretory protein delivery.
    Article Snippet: .. Segment 8 was amplified from the viral RNAs via segment-specific RT-PCR using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) and primers 4F and 4R (see Table S2). .. Amplicons were confirmed by Sanger sequencing (Eton Bioscience).

    One Step RT-PCR:

    Article Title: Re-engineering segment 8 facilitates generation of a versatile live-attenuated influenza A virus vector platform for secretory protein delivery.
    Article Snippet: .. Segment 8 was amplified from the viral RNAs via segment-specific RT-PCR using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) and primers 4F and 4R (see Table S2). .. Amplicons were confirmed by Sanger sequencing (Eton Bioscience).

    Platinum:

    Article Title: Re-engineering segment 8 facilitates generation of a versatile live-attenuated influenza A virus vector platform for secretory protein delivery.
    Article Snippet: .. Segment 8 was amplified from the viral RNAs via segment-specific RT-PCR using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) and primers 4F and 4R (see Table S2). .. Amplicons were confirmed by Sanger sequencing (Eton Bioscience).

    Purification:

    Article Title: Bivalent mRNA booster encoding virus-like particles elicits potent polyclass receptor-binding domain antibodies in pre-vaccinated mice
    Article Snippet: .. Briefly, Avi/His-tagged S trimers and His-tagged RBD and S2 proteins were purified from transiently transfected Expi293F cells (A14527; Thermo Fisher; authenticated by supplier; negative for mycoplasma) by nickel affinity chromatography (HisTrap, Cytiva) and size-exclusion chromatography (SEC) (Superose 6 Increase 10/300, Cytiva) ( ; ; ). ..

    Transfection:

    Article Title: Bivalent mRNA booster encoding virus-like particles elicits potent polyclass receptor-binding domain antibodies in pre-vaccinated mice
    Article Snippet: .. Briefly, Avi/His-tagged S trimers and His-tagged RBD and S2 proteins were purified from transiently transfected Expi293F cells (A14527; Thermo Fisher; authenticated by supplier; negative for mycoplasma) by nickel affinity chromatography (HisTrap, Cytiva) and size-exclusion chromatography (SEC) (Superose 6 Increase 10/300, Cytiva) ( ; ; ). ..

    Affinity Chromatography:

    Article Title: Bivalent mRNA booster encoding virus-like particles elicits potent polyclass receptor-binding domain antibodies in pre-vaccinated mice
    Article Snippet: .. Briefly, Avi/His-tagged S trimers and His-tagged RBD and S2 proteins were purified from transiently transfected Expi293F cells (A14527; Thermo Fisher; authenticated by supplier; negative for mycoplasma) by nickel affinity chromatography (HisTrap, Cytiva) and size-exclusion chromatography (SEC) (Superose 6 Increase 10/300, Cytiva) ( ; ; ). ..

    Size-exclusion Chromatography:

    Article Title: Bivalent mRNA booster encoding virus-like particles elicits potent polyclass receptor-binding domain antibodies in pre-vaccinated mice
    Article Snippet: .. Briefly, Avi/His-tagged S trimers and His-tagged RBD and S2 proteins were purified from transiently transfected Expi293F cells (A14527; Thermo Fisher; authenticated by supplier; negative for mycoplasma) by nickel affinity chromatography (HisTrap, Cytiva) and size-exclusion chromatography (SEC) (Superose 6 Increase 10/300, Cytiva) ( ; ; ). ..

    Concentration Assay:

    Article Title: Targeting the polyene chain represents an adjuvant strategy for optimizing polyene antifungals
    Article Snippet: The synergistic effect of adjuvants with PAs was measured by a chequerboard assay following the protocol from Clinical and Laboratory Standards Institute document M27 ( ). .. Strains ( SI Appendix , Table S2 ) were diluted to a final concentration of 5 × 10 3 cells/mL in RPMI 1640 medium (Gibco), RPMI 1640 medium supplemented with 10% fetal bovine serum, or interstitial fluid, and treated with PAs (0.125 to 8 μg/mL; AmB, candicidin, hamycin, mepartricin A, nystatin, filipin III, and natamycin) alone or in combination with adjuvants (0.25 to 16 μg/mL; vitamin D 3 , vitamin D 2 , 25(OH)D 3 , and alfacalcidol) for 48 h. The vehicle-treated strains were used as a growth reference. .. Growth inhibition was determined by a visual and multifunction microplate reader (Varioskan LUX, Thermo Scientific).

    Article Title: Multilevel synergistic mechanisms of a nanocarrier-enabled topramezone herbicide against invasive weeds.
    Article Snippet: .. To further examine biosafety at the cellular level, Drosophila S2 cells were cultured in Schneider’s Drosophila medium (Gibco, USA) supplemented with 10% fetal bovine serum at 28 °C, which were then seeded into 96-well plates at a density of 1 × 104 cells per well, and exposed to TOP@HLDP (mass ratio of 1:1.16; TOP concentration: 6, 9, 12, 15, 18, 21 and 24 mg/L), free TOP or ddH2O (control) for 24 h. For alive/dead cell discrimination, cells were further stained using Calcein-AM/propidium iodide (PI) dual-fluorescence viability kit according to the manufacturer’s instructions. ..

    Cell Culture:

    Article Title: Multilevel synergistic mechanisms of a nanocarrier-enabled topramezone herbicide against invasive weeds.
    Article Snippet: .. To further examine biosafety at the cellular level, Drosophila S2 cells were cultured in Schneider’s Drosophila medium (Gibco, USA) supplemented with 10% fetal bovine serum at 28 °C, which were then seeded into 96-well plates at a density of 1 × 104 cells per well, and exposed to TOP@HLDP (mass ratio of 1:1.16; TOP concentration: 6, 9, 12, 15, 18, 21 and 24 mg/L), free TOP or ddH2O (control) for 24 h. For alive/dead cell discrimination, cells were further stained using Calcein-AM/propidium iodide (PI) dual-fluorescence viability kit according to the manufacturer’s instructions. ..

    Control:

    Article Title: Multilevel synergistic mechanisms of a nanocarrier-enabled topramezone herbicide against invasive weeds.
    Article Snippet: .. To further examine biosafety at the cellular level, Drosophila S2 cells were cultured in Schneider’s Drosophila medium (Gibco, USA) supplemented with 10% fetal bovine serum at 28 °C, which were then seeded into 96-well plates at a density of 1 × 104 cells per well, and exposed to TOP@HLDP (mass ratio of 1:1.16; TOP concentration: 6, 9, 12, 15, 18, 21 and 24 mg/L), free TOP or ddH2O (control) for 24 h. For alive/dead cell discrimination, cells were further stained using Calcein-AM/propidium iodide (PI) dual-fluorescence viability kit according to the manufacturer’s instructions. ..

    Staining:

    Article Title: Multilevel synergistic mechanisms of a nanocarrier-enabled topramezone herbicide against invasive weeds.
    Article Snippet: .. To further examine biosafety at the cellular level, Drosophila S2 cells were cultured in Schneider’s Drosophila medium (Gibco, USA) supplemented with 10% fetal bovine serum at 28 °C, which were then seeded into 96-well plates at a density of 1 × 104 cells per well, and exposed to TOP@HLDP (mass ratio of 1:1.16; TOP concentration: 6, 9, 12, 15, 18, 21 and 24 mg/L), free TOP or ddH2O (control) for 24 h. For alive/dead cell discrimination, cells were further stained using Calcein-AM/propidium iodide (PI) dual-fluorescence viability kit according to the manufacturer’s instructions. ..

    In Vitro:

    Article Title: Germ cell-directed exosomal delivery enables in vivo genetic modification in birds.
    Article Snippet: .. ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Fig. S2 Lipid nanoparticle (LNP)-mediated gene delivery in vitro. (A) Cryo-electron microscopy (cryo-EM) images of LNP formulations encapsulating EGFP mRNA at different N/P ratios (4:1, 6:1, and 8:1). ..

    Cryo-Electron Microscopy:

    Article Title: Germ cell-directed exosomal delivery enables in vivo genetic modification in birds.
    Article Snippet: .. ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Fig. S2 Lipid nanoparticle (LNP)-mediated gene delivery in vitro. (A) Cryo-electron microscopy (cryo-EM) images of LNP formulations encapsulating EGFP mRNA at different N/P ratios (4:1, 6:1, and 8:1). ..

    Solvent:

    Article Title: Li-Doped Front Interface Engineering Enables Flexible Kesterite Solar Cell With 12.31% Efficiency and Module With 7.85% Efficiency.
    Article Snippet: Performance of flexible kesterite Cu2 ZnSn(S, Se)4 (CZTSSe) solar cell is mainly limited by large open-circuit voltage deficit ( Voc, def ) due to severe carrier recombination primarily caused by numerous defects at CZTSSe/CdS interface.. Here, a modification strategy by doping Li ions at front interface is proposed to reducing carrier recombination, where the sample uniformly doped Li ions into CZTSSe absorber is the reference group.. In this process, the secondary phases and defects in the CZTSSe absorber are effectively inhibited.



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    Image Search Results


    miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Journal: Bioactive Materials

    Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury

    doi: 10.1016/j.bioactmat.2026.06.009

    Figure Lengend Snippet: miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Article Snippet: The miRNA stem-loop primers (Shanghai Jierui Bioengineering) are listed in .

    Techniques: Functional Assay, Quantitative RT-PCR, Biomarker Discovery, Flow Cytometry, Cell Culture, Activity Assay, Expressing