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stem loop primers  (Eurofins)

 
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    Eurofins stem loop primers
    Stem Loop Primers, supplied by Eurofins, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stem+loop+primers/primers+qpcr+rt/pm41816892-202-4-9
    Average 86 stars, based on 1 article reviews
    stem loop primers - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Purification:

    Article Title: One-Pot RT-LAMP CRISPR/Cas12b Platform for Rapid Detection of Tilapia Lake Virus.
    Article Snippet: .. The RT- LAMP primers and sgRNA were chemically synthesised and purified by standard desalting for optimal performance by Eurofins Scientific and Integrated DNA Technologies (IDT), respectively. ..

    Quantitative RT-PCR:

    Article Title: Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment
    Article Snippet: .. RT-qPCR primers for the six reference genes were designed using the IDT PrimerQuest ToolTM and were synthesized by Eurofins, Bengaluru, India. .. RT-qPCR was performed using the CFX96 Touch Real-Time PCR system (Bio-Rad, USA) and TB GreenTM Premix Ex TaqTM II (Takara Biotechnology, Japan).

    Article Title: Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam tolerance and host-pathogen interactions.
    Article Snippet: .. 200 201 202 AR TIC LE IN PR ES S 10 203 Table 3 - Primers used to quantify the mRNA expression of target genes by 204 qRT-PCR in M. smegmatis. qRT-PCR primersa Target gene Primer sequences Length (bp) %GC Tmb (°C) Product size (bp) AEc (%) PFwd 5' - ACACCGACCTGGAACTCG - 3' 18 61 58sigA (MSMEG_2758) PRv 5' - GACGCCTTGTCCTTCTCG - 3' 18 61 58 152 80 PFwd 5' - GGTAAGCGGGTCATCATCG - 3' 19 58 59cwlM (MSMEG_6935) PRv 5' - GGTCCCACAGGATGTCG - 3' 17 65 58 104 108 205 a - qRT-PCR primers were designed to avoid primer secondary structures and sequence 206 repeats, have at least 50% of GC content, and have a Tm between 57-63°C; b - 207 Calculated with the Eurofins Genomics Tm formula; c – AE designates the amplification 208 efficiency calculated for each pair of primers in a single-run qPCR amplification of the 209 calibrator sample (M. smegmatis WT), performed in triplicates. ..

    Article Title: Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment.
    Article Snippet: .. RT-qPCR primers for the six reference genes were designed using the IDT PrimerQuest ToolTM and were synthesized by Eurofins, Bengaluru, India. .. RT-qPCR was performed using the CFX96 Touch Real-Time PCR system (Bio-Rad, USA) and TB GreenTM Premix Ex TaqTM II (Takara Biotechnology, Japan).

    Synthesized:

    Article Title: Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment
    Article Snippet: .. RT-qPCR primers for the six reference genes were designed using the IDT PrimerQuest ToolTM and were synthesized by Eurofins, Bengaluru, India. .. RT-qPCR was performed using the CFX96 Touch Real-Time PCR system (Bio-Rad, USA) and TB GreenTM Premix Ex TaqTM II (Takara Biotechnology, Japan).

    Article Title: Stability analysis of reference genes in Maconellicoccus hirsutus across various conditions including dsRNA treatment.
    Article Snippet: .. RT-qPCR primers for the six reference genes were designed using the IDT PrimerQuest ToolTM and were synthesized by Eurofins, Bengaluru, India. .. RT-qPCR was performed using the CFX96 Touch Real-Time PCR system (Bio-Rad, USA) and TB GreenTM Premix Ex TaqTM II (Takara Biotechnology, Japan).

    Expressing:

    Article Title: Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam tolerance and host-pathogen interactions.
    Article Snippet: .. 200 201 202 AR TIC LE IN PR ES S 10 203 Table 3 - Primers used to quantify the mRNA expression of target genes by 204 qRT-PCR in M. smegmatis. qRT-PCR primersa Target gene Primer sequences Length (bp) %GC Tmb (°C) Product size (bp) AEc (%) PFwd 5' - ACACCGACCTGGAACTCG - 3' 18 61 58sigA (MSMEG_2758) PRv 5' - GACGCCTTGTCCTTCTCG - 3' 18 61 58 152 80 PFwd 5' - GGTAAGCGGGTCATCATCG - 3' 19 58 59cwlM (MSMEG_6935) PRv 5' - GGTCCCACAGGATGTCG - 3' 17 65 58 104 108 205 a - qRT-PCR primers were designed to avoid primer secondary structures and sequence 206 repeats, have at least 50% of GC content, and have a Tm between 57-63°C; b - 207 Calculated with the Eurofins Genomics Tm formula; c – AE designates the amplification 208 efficiency calculated for each pair of primers in a single-run qPCR amplification of the 209 calibrator sample (M. smegmatis WT), performed in triplicates. ..

    Sequencing:

    Article Title: Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam tolerance and host-pathogen interactions.
    Article Snippet: .. 200 201 202 AR TIC LE IN PR ES S 10 203 Table 3 - Primers used to quantify the mRNA expression of target genes by 204 qRT-PCR in M. smegmatis. qRT-PCR primersa Target gene Primer sequences Length (bp) %GC Tmb (°C) Product size (bp) AEc (%) PFwd 5' - ACACCGACCTGGAACTCG - 3' 18 61 58sigA (MSMEG_2758) PRv 5' - GACGCCTTGTCCTTCTCG - 3' 18 61 58 152 80 PFwd 5' - GGTAAGCGGGTCATCATCG - 3' 19 58 59cwlM (MSMEG_6935) PRv 5' - GGTCCCACAGGATGTCG - 3' 17 65 58 104 108 205 a - qRT-PCR primers were designed to avoid primer secondary structures and sequence 206 repeats, have at least 50% of GC content, and have a Tm between 57-63°C; b - 207 Calculated with the Eurofins Genomics Tm formula; c – AE designates the amplification 208 efficiency calculated for each pair of primers in a single-run qPCR amplification of the 209 calibrator sample (M. smegmatis WT), performed in triplicates. ..

    Amplification:

    Article Title: Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam tolerance and host-pathogen interactions.
    Article Snippet: .. 200 201 202 AR TIC LE IN PR ES S 10 203 Table 3 - Primers used to quantify the mRNA expression of target genes by 204 qRT-PCR in M. smegmatis. qRT-PCR primersa Target gene Primer sequences Length (bp) %GC Tmb (°C) Product size (bp) AEc (%) PFwd 5' - ACACCGACCTGGAACTCG - 3' 18 61 58sigA (MSMEG_2758) PRv 5' - GACGCCTTGTCCTTCTCG - 3' 18 61 58 152 80 PFwd 5' - GGTAAGCGGGTCATCATCG - 3' 19 58 59cwlM (MSMEG_6935) PRv 5' - GGTCCCACAGGATGTCG - 3' 17 65 58 104 108 205 a - qRT-PCR primers were designed to avoid primer secondary structures and sequence 206 repeats, have at least 50% of GC content, and have a Tm between 57-63°C; b - 207 Calculated with the Eurofins Genomics Tm formula; c – AE designates the amplification 208 efficiency calculated for each pair of primers in a single-run qPCR amplification of the 209 calibrator sample (M. smegmatis WT), performed in triplicates. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam tolerance and host-pathogen interactions.
    Article Snippet: .. 200 201 202 AR TIC LE IN PR ES S 10 203 Table 3 - Primers used to quantify the mRNA expression of target genes by 204 qRT-PCR in M. smegmatis. qRT-PCR primersa Target gene Primer sequences Length (bp) %GC Tmb (°C) Product size (bp) AEc (%) PFwd 5' - ACACCGACCTGGAACTCG - 3' 18 61 58sigA (MSMEG_2758) PRv 5' - GACGCCTTGTCCTTCTCG - 3' 18 61 58 152 80 PFwd 5' - GGTAAGCGGGTCATCATCG - 3' 19 58 59cwlM (MSMEG_6935) PRv 5' - GGTCCCACAGGATGTCG - 3' 17 65 58 104 108 205 a - qRT-PCR primers were designed to avoid primer secondary structures and sequence 206 repeats, have at least 50% of GC content, and have a Tm between 57-63°C; b - 207 Calculated with the Eurofins Genomics Tm formula; c – AE designates the amplification 208 efficiency calculated for each pair of primers in a single-run qPCR amplification of the 209 calibrator sample (M. smegmatis WT), performed in triplicates. ..

    Article Title: Differential Expression of Defensins and Other Innate Immune Effectors by Indian Isolates of Chikungunya Virus.
    Article Snippet: Chikungunya virus (CHIKV) infection has been associated with heightened disease burden and fatality rate, especially post its resurgence in 2006, with presentation of various atypical symptoms.. Its re‐emergence and the subsequent periodic outbreaks indicate the constant evolution of the virus due to the incorporation of mutations in its genome.. This can potentially elicit a differential induction of innate immune response of varying degrees, which has been poorly studied in the case of CHIKV.

    Article Title: Understanding Plasmodium vivax recurrent infections using an amplicon deep sequencing assay, identity-by-descent and model-based classification
    Article Snippet: PvAmpSeq Primers/Probes (Primary, Secondary, Index) , Eurofins , Sequences provided in Zenodo: https://doi.org/10.5281/zenodo.17474689. .. Duplex TaqMan qPCR Primers/Probes (Pv and Pf 18s rRNA) , Eurofins , Sequences listed in ref. 36. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Sulfated glycosaminoglycans inhibit LCMV entry and modulate antiviral immunity and pathology.
    Article Snippet: In case of in vivo experiments, organs were extracted into lysis buffer from RNeasy Kit (QIAGEN), homogenized using a Tissue Lyser (QIAGEN), and total cellular RNA was purified using RNeasy Kit according to the manufacturer’s protocol. mRNA expression levels were analyzed using iTaq Universal SYBR Green 1-Step Kit (Bio-Rad) according to the manufacturer’s protocol. .. RT-PCR primers were obtained from Eurofins Scientific (Table 1). ..



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    Image Search Results


    miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Journal: Bioactive Materials

    Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury

    doi: 10.1016/j.bioactmat.2026.06.009

    Figure Lengend Snippet: miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Article Snippet: The miRNA stem-loop primers (Shanghai Jierui Bioengineering) are listed in .

    Techniques: Functional Assay, Quantitative RT-PCR, Biomarker Discovery, Flow Cytometry, Cell Culture, Activity Assay, Expressing