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stedycon smart control software  (abberior instruments)

 
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    Structured Review

    abberior instruments stedycon smart control software
    Stedycon Smart Control Software, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stedycon/stedycon+software/pm36913580-303-1-0
    Average 90 stars, based on 1 article reviews
    stedycon smart control software - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Software:

    Article Title: Structural heterogeneity of the ion and lipid channel TMEM16F
    Article Snippet: Stimulated emission depletion (STED) imaging and confocal z-sectioning was performed with a STEDYCON microscope (Abberior GmbH). .. Images were acquired with NIS-Elements (Nikon) and STEDYCON (Abberior GmbH) acquisition software and analyzed with ImageJ 1.47 v (NIH) and Image Gallery 9 (Abberior GmbH). ..

    Article Title: In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells
    Article Snippet: STED imaging was performed on the Abberior STEDYCON coupled to a Leica SP5 II confocal microscope equipped with a 100×/1.44 NA oil immersion lens. .. Abberior STEDYCON smart control software was used for imaging with the STED 775-nm laser line, set at 42-nm resolution, and a fixed pixel size of 25 nm. .. Cells were immune-stained with an anti-GFP primary antibody (Abcam, ab6556;1:100 dilution) followed by a ATTO647N goat anti-rabbit IgG (Sigma, 18373; 1:50 dilution).

    Article Title: A lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins.
    Article Snippet: Acute lysosomal membrane damage reduces the cellular population of functional lysosomes.. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in cells depleted in TFEB and TFE3.. We combined proximity-labelling-based proteomics, biochemistry and high-resolution microscopy to unravel a lysosomal membrane regeneration pathway that depends on ATG8, the lysosomal membrane protein LIMP2, the RAB7 GTPase-activating protein TBC1D15 and proteins required for autophagic lysosomal reformation, including dynamin-2, kinesin-5B and clathrin.

    Article Title: Layer 5 myelination gates corticothalamic coincidence detection
    Article Snippet: .. Nodes of Ranvier were imaged with a Zeiss Axiovert 200M controlled by STEDYCON software (Version 9, Abberior Instruments, Göttingen, Germany). ..

    Article Title: Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin
    Article Snippet: .. All acquisition operations were controlled by the STEDYCON Software (Abberior Instruments). by Bitplane. .. STED images of OVSAHO cells were filmed using an Expert Line easy3D STED microscope system (Abberior Instruments) with the 100 x/1.4NA UPLSAPO100x oil objective (Olympus) and an avalanche photodiode detector (APD).

    Article Title: Structural heterogeneity of the ion and lipid channel TMEM16F.
    Article Snippet: Stimulated emission depletion (STED) imaging and confocal z-sectioning was performed with a STEDYCON microscope (Abberior GmbH). .. Images were acquired with NIS-Elements (Nikon) and STEDYCON (Abberior GmbH) acquisition software and analyzedwith ImageJ 1.47 v (NIH) and ImageGallery 9 (AbberiorGmbH). ..

    Control:

    Article Title: In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells
    Article Snippet: STED imaging was performed on the Abberior STEDYCON coupled to a Leica SP5 II confocal microscope equipped with a 100×/1.44 NA oil immersion lens. .. Abberior STEDYCON smart control software was used for imaging with the STED 775-nm laser line, set at 42-nm resolution, and a fixed pixel size of 25 nm. .. Cells were immune-stained with an anti-GFP primary antibody (Abcam, ab6556;1:100 dilution) followed by a ATTO647N goat anti-rabbit IgG (Sigma, 18373; 1:50 dilution).

    Article Title: In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells.
    Article Snippet: STED imaging was performed on the Abberior STEDYCON coupled to a Leica SP5 II confocal microscope equipped with a 100×/1.44 NA oil immersion lens. .. Abberior STEDYCON smart control software was used for imaging with the STED 775- nm laser line, set at 42-nm resolution, and a fixed pixel size of 25 nm. .. Cells were immune-stained with an anti-GFP primary antibody (Abcam, ab6556;1:100 dilution) followed by a ATTO647N goat anti-rabbit IgG (Sigma, 18373; 1:50 dilution).

    Imaging:

    Article Title: In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells
    Article Snippet: STED imaging was performed on the Abberior STEDYCON coupled to a Leica SP5 II confocal microscope equipped with a 100×/1.44 NA oil immersion lens. .. Abberior STEDYCON smart control software was used for imaging with the STED 775-nm laser line, set at 42-nm resolution, and a fixed pixel size of 25 nm. .. Cells were immune-stained with an anti-GFP primary antibody (Abcam, ab6556;1:100 dilution) followed by a ATTO647N goat anti-rabbit IgG (Sigma, 18373; 1:50 dilution).

    Article Title: In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells.
    Article Snippet: STED imaging was performed on the Abberior STEDYCON coupled to a Leica SP5 II confocal microscope equipped with a 100×/1.44 NA oil immersion lens. .. Abberior STEDYCON smart control software was used for imaging with the STED 775- nm laser line, set at 42-nm resolution, and a fixed pixel size of 25 nm. .. Cells were immune-stained with an anti-GFP primary antibody (Abcam, ab6556;1:100 dilution) followed by a ATTO647N goat anti-rabbit IgG (Sigma, 18373; 1:50 dilution).



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    Image Search Results


    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Journal: bioRxiv

    Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

    doi: 10.1101/2025.07.09.663961

    Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

    Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay