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stedycon  (Carl Zeiss)


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    Structured Review

    Carl Zeiss stedycon
    Stedycon, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stedycon/stedycon/pm40128797-286-11-16
    Average 90 stars, based on 1 article reviews
    stedycon - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel.
    Article Snippet: Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments GmbH, Göttingen) with excitation lasers at 488, 561, and 640 nm, and a STED laser at 775 nm (maximum intensity 1.25 W; all lasers are pulsed with 40 MHz repetition rate). .. The STEDYCON was mounted on the camera port of an AxioImager Z2 H. Bahri et al. BBA - Molecular Cell Research 1868 (2021) 118942 upright microscope (Zeiss, Jena, Germany), equipped with a 100× objective (alpha Plan-Apochromat, Oil, DIC, Vis, NA 1.46; Zeiss). ..

    Article Title: Dynamic Regulation of Synaptopodin and the Axon Initial Segment in Retinal Ganglion Cells During Postnatal Development.
    Article Snippet: Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments GmbH, Göttingen) with excitation lasers at 488, 561, and 640 nm, and a STED laser at 775 nm (maximum intensity 1.25 W; all lasers are pulsed with 40 MHz repetition rate). .. The STEDYCON was mounted on the camera port of an AxioImager.Z2 upright microscope (Zeiss, Jena, Germany), equipped with a 100x objective (alpha Plan-Apochromat, Oil, DIC, Vis, NA 1.46; Zeiss). ..

    Article Title: TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel
    Article Snippet: Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments GmbH, Göttingen) with excitation lasers at 488, 561, and 640 nm, and a STED laser at 775 nm (maximum intensity 1.25 W; all lasers are pulsed with 40 MHz repetition rate). .. The STEDYCON was mounted on the camera port of an AxioImager Z2 upright microscope (Zeiss, Jena, Germany), equipped with a 100x objective (alpha Plan-Apochromat, Oil, DIC, Vis, NA 1.46; Zeiss). ..

    Article Title: Functional genomic profiling of O-GlcNAc reveals its context-specific interplay with RNA polymerase II.
    Article Snippet: Cells were washed once with PBS 1X, excess of solution was drained and coverslips were properly mounted with Abberior Mount Solid Antifade (Abberior #MM- 2013- 2X15ML). .. Imaging by stimulated emission depletion (STED) microscopy was performed using a STEDYCON mounted on an upright Zeiss microscope in confocal or STED modes. .. Samples were imaged with a Zeiss 100 Å ~ 1.46 NA objective, 20 nm pixel size, 5 μs pixel dwell time, 15-line accumulations, and a pinhole of 64 μm.

    Imaging:

    Article Title: Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics
    Article Snippet: The images were captured either by an sCMOS camera (C14440-20UP, Hamamatsu) or an EMCCD camera (iXon3 897, Andor). .. Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments) with excitation lasers at 450 nm, 594 nm, and 640 nm and a STED laser at 775 nm wavelength (all pulsed), and it was controlled by its own software, Smart Control (SN: SY210901). .. The STEDYCON was mounted at the camera port of a Zeiss Axio Observer Inverted microscope equipped with a ×100/1.40 oil, UPlanSApo objective.

    Article Title: Functional genomic profiling of O-GlcNAc reveals its context-specific interplay with RNA polymerase II.
    Article Snippet: Cells were washed once with PBS 1X, excess of solution was drained and coverslips were properly mounted with Abberior Mount Solid Antifade (Abberior #MM- 2013- 2X15ML). .. Imaging by stimulated emission depletion (STED) microscopy was performed using a STEDYCON mounted on an upright Zeiss microscope in confocal or STED modes. .. Samples were imaged with a Zeiss 100 Å ~ 1.46 NA objective, 20 nm pixel size, 5 μs pixel dwell time, 15-line accumulations, and a pinhole of 64 μm.

    Software:

    Article Title: Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics
    Article Snippet: The images were captured either by an sCMOS camera (C14440-20UP, Hamamatsu) or an EMCCD camera (iXon3 897, Andor). .. Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments) with excitation lasers at 450 nm, 594 nm, and 640 nm and a STED laser at 775 nm wavelength (all pulsed), and it was controlled by its own software, Smart Control (SN: SY210901). .. The STEDYCON was mounted at the camera port of a Zeiss Axio Observer Inverted microscope equipped with a ×100/1.40 oil, UPlanSApo objective.

    Control:

    Article Title: Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics
    Article Snippet: The images were captured either by an sCMOS camera (C14440-20UP, Hamamatsu) or an EMCCD camera (iXon3 897, Andor). .. Stimulated Emission Depletion (STED) imaging was performed using a STEDYCON (Abberior Instruments) with excitation lasers at 450 nm, 594 nm, and 640 nm and a STED laser at 775 nm wavelength (all pulsed), and it was controlled by its own software, Smart Control (SN: SY210901). .. The STEDYCON was mounted at the camera port of a Zeiss Axio Observer Inverted microscope equipped with a ×100/1.40 oil, UPlanSApo objective.



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    Nikon nikon stedycon microscope operating
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    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x <t>STED</t> immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001
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    Image Search Results


    (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Journal: bioRxiv

    Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

    doi: 10.1101/2025.07.09.663961

    Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

    Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

    Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay