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abberior instruments abberior stedycon
Abberior Stedycon, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stedycon/abberior+stedycon/pm38634253-323-58-60
Average 90 stars, based on 1 article reviews
abberior stedycon - by Bioz Stars, 2026-09
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Images

Related Articles

Microscopy:

Article Title: Early resolution of sister chromatids during C. elegans meiosis
Article Snippet: .. 2D STED images were acquired as in ( Almanzar et al . 2023 ), using Abberior STEDYCON mounted on a Nikon Eclipse Ti microscope body with a 100x 1.45 NA oil immersion objective. ..

Article Title: Distinct input-specific mechanisms enable presynaptic homeostatic plasticity.
Article Snippet: .. Briefly, STED imaging was performed using an Abberior STEDYCON system mounted on a Nikon Eclipse FN1 upright microscope equipped with four excitation lasers (640, 561, 488, and 405 nm), a pulsed STED laser at 775 nm, and three avalanche photodiode detectors that operate in a single photon counting mode. .. Multichannel 2D STED images were acquired using a 100× Nikon Plan APO 1.45 NA oil immersion objective with a 15- nm fixed pixel size and a 10- μs dwell time using 15× line accumulation in photon counting mode and a field of view of one to two boutons.

Article Title: The synaptonemal complex stabilizes meiosis in allotetraploid Brassica napus and autotetraploid Arabidopsis thaliana
Article Snippet: The slides were examined under the SOPTOP RX50 fluorescence microscope (Sunny Optical Technology, Ningbo, China) equipped with a ×100 oil immersion objective. .. For the STED images of ASY1 in zyp1 mutants, Abberior STAR Red (Goat anti‐Rat) was used as the secondary antibody, and images were capture using an Olympus IX81 fluorescence microscope (Tokyo, Japan) equipped with an Abberior STEDYCON (Göttingen, Germany). ..

Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The cells were then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and stained with nephrin and phalloidin-488. .. Images were obtained using Stedycon Abberior (Göttingen, Germany) and Olympus 1X81 (Tokyo, Japan) confocal microscope available at the Advanced Light Microscopy Core Facility at the Colorado University Anschutz Medical Campus. ..

Imaging:

Article Title: Distinct input-specific mechanisms enable presynaptic homeostatic plasticity.
Article Snippet: .. Briefly, STED imaging was performed using an Abberior STEDYCON system mounted on a Nikon Eclipse FN1 upright microscope equipped with four excitation lasers (640, 561, 488, and 405 nm), a pulsed STED laser at 775 nm, and three avalanche photodiode detectors that operate in a single photon counting mode. .. Multichannel 2D STED images were acquired using a 100× Nikon Plan APO 1.45 NA oil immersion objective with a 15- nm fixed pixel size and a 10- μs dwell time using 15× line accumulation in photon counting mode and a field of view of one to two boutons.

Article Title: Harnessing an MMP-Independent NIR Probe Unveiling the Different Mitochondrial Cristae Changes during Mitophagy and Ferroptosis under STED Microscopy
Article Snippet: .. For co-localization imaging experiments, live cells were incubated with commercial dyes for 10 minutes, then incubated with CSA or CSN for 30 minutes, then washed twice with 1 mL PBS, and finally imaged with Abberior STEDYCON. ..

Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth
Article Snippet: Slides were imaged using a Zeiss spinning disk and a BC43 spinning disk confocal microscopes, or using Keyence BZ-X810 for widefield microscopy. .. For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit. .. Images were acquired as single plane to obtain maximal X,Y resolution (∼30nm), using a Leica HC PL APO 100x/140 oil CS2 objective.

Fluorescence:

Article Title: The synaptonemal complex stabilizes meiosis in allotetraploid Brassica napus and autotetraploid Arabidopsis thaliana
Article Snippet: The slides were examined under the SOPTOP RX50 fluorescence microscope (Sunny Optical Technology, Ningbo, China) equipped with a ×100 oil immersion objective. .. For the STED images of ASY1 in zyp1 mutants, Abberior STAR Red (Goat anti‐Rat) was used as the secondary antibody, and images were capture using an Olympus IX81 fluorescence microscope (Tokyo, Japan) equipped with an Abberior STEDYCON (Göttingen, Germany). ..

Light Microscopy:

Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The cells were then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and stained with nephrin and phalloidin-488. .. Images were obtained using Stedycon Abberior (Göttingen, Germany) and Olympus 1X81 (Tokyo, Japan) confocal microscope available at the Advanced Light Microscopy Core Facility at the Colorado University Anschutz Medical Campus. ..

Incubation:

Article Title: Harnessing an MMP-Independent NIR Probe Unveiling the Different Mitochondrial Cristae Changes during Mitophagy and Ferroptosis under STED Microscopy
Article Snippet: .. For co-localization imaging experiments, live cells were incubated with commercial dyes for 10 minutes, then incubated with CSA or CSN for 30 minutes, then washed twice with 1 mL PBS, and finally imaged with Abberior STEDYCON. ..

other:

Article Title: miR-204-5p Protects Nephrin from Enzymatic Degradation in Cultured Mouse Podocytes Treated with Nephrotoxic Serum.
Article Snippet: The images were obtained using a Stedycon Abberior (STED) (Göttingen, Germany) and Olympus 1X81(Tokyo, Japan) confocal microscope and 100X objective lens.



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Image Search Results


(A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Journal: bioRxiv

Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

doi: 10.1101/2025.07.09.663961

Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay