dna polymerase i (New England Biolabs)
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New England Biolabs
dna polymerase i
Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+program+stata+version+12/DNA+Polymerase+I/us08557522-1051-33-36
Average 99 stars, based on 10189 article reviews
Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+program+stata+version+12/DNA+Polymerase+I/us08557522-1051-33-36
Average 99 stars, based on 10189 article reviews
dna polymerase i - by Bioz Stars,
2026-10
99/100 stars
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Reverse Transcription:Article Title: Macrophage MERTK restrains GRAMD1A-driven mitochondrial oxysterol trafficking to limit colitis Article Snippet: Poly(A) RNA was purified from 1 μg total RNA using Dynabeads Oligo(dT) (Thermo Fisher) through two rounds of purification, followed by fragmentation with the Magnesium RNA Fragmentation Module (NEB) at 94 °C for 5-7 min. .. The fragmented RNA was reverse-transcribed into cDNA using SuperScriptTM II Reverse Transcriptase (Invitrogen) and subsequently used to synthesize U-labeled second-strand DNA with Article Title: From disease model to therapeutic insight: An engineered hydrogel reveals the role of matrix viscous dissipation in intervertebral disc degeneration Article Snippet: .. The resulting RNA fragments were reverse-transcribed to create cDNA using SuperScriptTM Reverse Transcriptase (Invitrogen, USA), which was subsequently used to synthesize U-labeled second-stranded DNAs with Article Title: Screening of M6a methylated genes associated with preovulatory follicle development in Bashang long-tailed chicken Article Snippet: The cleaved RNA fragments were incubated for 2 h at 4°C with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630). .. The immunoprecipitated RNA was reverse-transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), followed by synthesis of U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat. m0209, USA), cDNA Synthesis:Article Title: Integrated transcriptomic, proteomic, and metabolomic analyses reveal molecular mechanisms underlying clutch length differences in goose ovaries Article Snippet: The purified mRNA was then fragmented and reverse-transcribed into first-strand cDNA using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific, MA, USA). .. Second-strand cDNA synthesis was performed incorporating dUTP (Thermo Fisher Scientific, MA, USA), Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet Article Snippet: First-strand cDNA synthesis was conducted with random primers and Superscript II Reverse Transcriptase (catalog no. 18064014, Invitrogen, Carlsbad, CA, USA). .. Subsequently, second-strand cDNA synthesis was performed, employing Immunoprecipitation:Article Title: Screening of M6a methylated genes associated with preovulatory follicle development in Bashang long-tailed chicken Article Snippet: The cleaved RNA fragments were incubated for 2 h at 4°C with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630). .. The immunoprecipitated RNA was reverse-transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), followed by synthesis of U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat. m0209, USA), Polymerase Chain Reaction:Article Title: Engineering of the glucose uptake system to increase 2,4-Dihydroxybutyric acid production in Escherichia coli Article Snippet: The kits for plasmid DNA isolation and PCR clean-up were purchased from New England Biolabs (Frankfurt am Main, Germany) and used according to the instructions of the manufacturer. .. PCR reactions were performed with Subcloning:Article Title: Engineering of the glucose uptake system to increase 2,4-Dihydroxybutyric acid production in Escherichia coli Article Snippet: The kits for plasmid DNA isolation and PCR clean-up were purchased from New England Biolabs (Frankfurt am Main, Germany) and used according to the instructions of the manufacturer. .. PCR reactions were performed with |