ptyb 12 vector (New England Biolabs)
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New England Biolabs
ptyb 12 vector
Ptyb 12 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+program+stata+version+12/EcoRI/pmc04933167-156-19-27
Average 99 stars, based on 14890 article reviews
Ptyb 12 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 14890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+program+stata+version+12/EcoRI/pmc04933167-156-19-27
Average 99 stars, based on 14890 article reviews
ptyb 12 vector - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2314 (Ditty et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Article Title: Pan-cancer analysis identifies FKBP10 as a regulator of tumor immunosuppression and therapeutic response Article Snippet: .. The full-length human FKBP10 genetic sequence was amplified by PCR from a cDNA library using Phanta Super-Fidelity DNA Polymerase (Vazyme, China) and inserted into the LV-ECMV-PURO viral vector via the Bam HI and Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2991 (Ivleva et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Amplification:Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2314 (Ditty et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Article Title: Pan-cancer analysis identifies FKBP10 as a regulator of tumor immunosuppression and therapeutic response Article Snippet: .. The full-length human FKBP10 genetic sequence was amplified by PCR from a cDNA library using Phanta Super-Fidelity DNA Polymerase (Vazyme, China) and inserted into the LV-ECMV-PURO viral vector via the Bam HI and Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2991 (Ivleva et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Plasmid Preparation:Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2314 (Ditty et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2991 (Ivleva et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Purification:Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2314 (Ditty et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism Article Snippet: The neutral site 1 (NSI) plasmid pAM2991 (Ivleva et al. ) was linearized with EcoRI-HF (New England Biolabs) restriction enzyme. .. The PCR amplified gene fragment, EYFP DNA fragment and Transformation Assay:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Clone Assay:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Article Title: Isolation and characterization of Avs-1, a bacteriophage effective against the aquaculture pathogen Aeromonas veronii . Article Snippet: .. Putative endolysin gene in phage Lys40 was cloned into the vector pET28a via Hind III (New England Biolabs, Ipswich, MA, USA) and Sequencing:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Article Title: Pan-cancer analysis identifies FKBP10 as a regulator of tumor immunosuppression and therapeutic response Article Snippet: .. The full-length human FKBP10 genetic sequence was amplified by PCR from a cDNA library using Phanta Super-Fidelity DNA Polymerase (Vazyme, China) and inserted into the LV-ECMV-PURO viral vector via the Bam HI and shRNA:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Construct:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Generated:Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia. Article Snippet: Using pLJM1-Smc3 as template, the K38A mutation was introduced into Smc3 to generate pLJM1-Smc3_K38A via PCR (NEB #M0492) site-directed mutagenesis and Gibson Assembly (NEB #E2621). .. The reaction was then transformed into chemically competent E. coli (NEB #C3040) and positive clones were identified by Nanopore whole-plasmid sequencing (Plasmidsaurus). shRNA-expressing constructs was generated as follows: SGEP (Addgene #111170) or pLKO.1-puro (Addgene #8453) plasmids were subjected to Article Title: DNA supercoiling modulates bZIP transcription factor–DNA interaction Article Snippet: The commercial plasmid pUC19 (2686 bp) was isolated from E. coli using the PureLink HiPure Midiprep kit (Thermo Fisher Scientific). .. Nicked open-circular DNA was generated by incubating pDNA(SC-) (1 μg) with 1 U mg-1 of the strand-specific nicking endonuclease Nt.BspQI (New England Biolabs) in 1 x Nt.BspQI buffer at 50° C for 2 h. Linear DNA was produced by complete digestion of pDNA(SC-) with the cDNA Library Assay:Article Title: Pan-cancer analysis identifies FKBP10 as a regulator of tumor immunosuppression and therapeutic response Article Snippet: .. The full-length human FKBP10 genetic sequence was amplified by PCR from a cDNA library using Phanta Super-Fidelity DNA Polymerase (Vazyme, China) and inserted into the LV-ECMV-PURO viral vector via the Bam HI and Expressing:Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Article Title: Isolation and characterization of Avs-1, a bacteriophage effective against the aquaculture pathogen Aeromonas veronii . Article Snippet: .. Putative endolysin gene in phage Lys40 was cloned into the vector pET28a via Hind III (New England Biolabs, Ipswich, MA, USA) and Produced:Article Title: DNA supercoiling modulates bZIP transcription factor–DNA interaction Article Snippet: The commercial plasmid pUC19 (2686 bp) was isolated from E. coli using the PureLink HiPure Midiprep kit (Thermo Fisher Scientific). .. Nicked open-circular DNA was generated by incubating pDNA(SC-) (1 μg) with 1 U mg-1 of the strand-specific nicking endonuclease Nt.BspQI (New England Biolabs) in 1 x Nt.BspQI buffer at 50° C for 2 h. Linear DNA was produced by complete digestion of pDNA(SC-) with the Control:Article Title: Isolation and characterization of Avs-1, a bacteriophage effective against the aquaculture pathogen Aeromonas veronii . Article Snippet: .. Putative endolysin gene in phage Lys40 was cloned into the vector pET28a via Hind III (New England Biolabs, Ipswich, MA, USA) and |