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molecular biology nebnext ultra ii dna library prep kit for illumina  (New England Biolabs)


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    New England Biolabs molecular biology nebnext ultra ii dna library prep kit for illumina
    Molecular Biology Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/statistical+program+stata+version+12/NEBNext+Ultra+II+DNA+Library+Prep+Kit+for+Illumina/pm31943854-36-52-54
    Average 99 stars, based on 11439 article reviews
    molecular biology nebnext ultra ii dna library prep kit for illumina - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    DNA Library Preparation:

    Article Title: Outbreaks of human respiratory syncytial virus in wild gorillas highlight the importance of prevention measures and integrated surveillance for risk mitigation
    Article Snippet: .. Dual-index libraries were built from double stranded cDNA using the NEBNext Ultra II DNA library preparation kit (New England Biolabs). ..

    Construct:

    Article Title: N-acetylated chito-oligosaccharides and xylanase improve intestinal health and production performance in laying hens
    Article Snippet: The Agilent Bioanalyzer 2100 system and the Qubit@ 2.0 Fluorometer (Thermo Scientific) were used to evaluate the library's quality. (iv) Sequencing data processing: by coupling particular primers with barcodes, PCR was carried out. .. Using the NEBNext® UltraTM IIDNA Library Prep Kit (Cat No. E7645), the DNA libraries were constructed. .. Then, using the Illumina paired-end platform (Novaseq6000 platform PE250), 250 bp paired-end raw reads were generated by end repairing, adding A to tails, purification, and amplicon sequencing.

    Article Title: Tannic acid supplementation exerts biphasic effects on growth performance, immune function, and gut microbiota in Pekin duck
    Article Snippet: .. Sequencing libraries were constructed using the NEBNext® UltraTM II DNA Library Prep Kit (E7645) according to the manufacturer's instructions. .. Quality assessment was performed using a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100.

    Sequencing:

    Article Title: Tannic acid supplementation exerts biphasic effects on growth performance, immune function, and gut microbiota in Pekin duck
    Article Snippet: .. Sequencing libraries were constructed using the NEBNext® UltraTM II DNA Library Prep Kit (E7645) according to the manufacturer's instructions. .. Quality assessment was performed using a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100.

    Article Title: Interactions of outer membrane lipoproteins P. aeruginosa PA3214 and E. coli PqiC with their MCE protein binding partners, PA3213 and PqiB
    Article Snippet: .. Following PCR, DNA fragments were pooled in equimolar amounts to generate the unselected and selected samples for amplicon sequencing with Illumina MiSeq 2 x 250 paired-end sequencing (NEBNext Ultra II Library Prep kit (New England Biologs #E7645) used to generate libraries for sequencing). ..

    Article Title: Comprehensive genome sequencing associates elevated mitochondrial DNA and homoplasmy with early stage I of pancreatic cancer
    Article Snippet: .. Briefly, cfDNA was isolated using the Qiagen circulating nucleic acid kit (cat# 55114) used in Olou et al., and NGS libraries were prepared for sequencing using New England Bioloab’s (NEB) NEBNext Ultra II DNA library prep for Illumina (cat. #E7645, NEB, Ipswitch, MA, USA). .. Sample libraries were sequenced on the Illumina NextSeq (150 bp paired-end sequencing) to an average read depth of 45.5 million reads.

    Software:

    Article Title: Shotgun sequencing data and SSR mining data of aibika ( Abelmoschus manihot , Malvaceae)
    Article Snippet: Type of data , Raw sequencing reads (fastq files), Tables (genome assembly and primers data), Figure (factorial analysis). .. Data collection , Genomic DNA was extracted from dried leaves of aibika accessions. DNA library was prepared using the NEBNext® UltraTM II DNA Library Prep kit and sequenced in paired-end with an Illumina MiSeq system. Sequences were assembled using ABySS software. SSR loci were found using the MISA Perl script. Primers were designed using Primer3 software. The contigs were blasted with NCBI-BLAST on okra reference genome. Candidate SSRs were selected based on motif type, repeat length, amplicon size and occurrence blast. Polymerase chain reaction (PCR) products were analyzed on an ABI 3500 xL Genetic Analyzer.. ..

    Amplification:

    Article Title: Shotgun sequencing data and SSR mining data of aibika ( Abelmoschus manihot , Malvaceae)
    Article Snippet: Type of data , Raw sequencing reads (fastq files), Tables (genome assembly and primers data), Figure (factorial analysis). .. Data collection , Genomic DNA was extracted from dried leaves of aibika accessions. DNA library was prepared using the NEBNext® UltraTM II DNA Library Prep kit and sequenced in paired-end with an Illumina MiSeq system. Sequences were assembled using ABySS software. SSR loci were found using the MISA Perl script. Primers were designed using Primer3 software. The contigs were blasted with NCBI-BLAST on okra reference genome. Candidate SSRs were selected based on motif type, repeat length, amplicon size and occurrence blast. Polymerase chain reaction (PCR) products were analyzed on an ABI 3500 xL Genetic Analyzer.. ..

    Article Title: Interactions of outer membrane lipoproteins P. aeruginosa PA3214 and E. coli PqiC with their MCE protein binding partners, PA3213 and PqiB
    Article Snippet: .. Following PCR, DNA fragments were pooled in equimolar amounts to generate the unselected and selected samples for amplicon sequencing with Illumina MiSeq 2 x 250 paired-end sequencing (NEBNext Ultra II Library Prep kit (New England Biologs #E7645) used to generate libraries for sequencing). ..

    Polymerase Chain Reaction:

    Article Title: Shotgun sequencing data and SSR mining data of aibika ( Abelmoschus manihot , Malvaceae)
    Article Snippet: Type of data , Raw sequencing reads (fastq files), Tables (genome assembly and primers data), Figure (factorial analysis). .. Data collection , Genomic DNA was extracted from dried leaves of aibika accessions. DNA library was prepared using the NEBNext® UltraTM II DNA Library Prep kit and sequenced in paired-end with an Illumina MiSeq system. Sequences were assembled using ABySS software. SSR loci were found using the MISA Perl script. Primers were designed using Primer3 software. The contigs were blasted with NCBI-BLAST on okra reference genome. Candidate SSRs were selected based on motif type, repeat length, amplicon size and occurrence blast. Polymerase chain reaction (PCR) products were analyzed on an ABI 3500 xL Genetic Analyzer.. ..

    Article Title: Interactions of outer membrane lipoproteins P. aeruginosa PA3214 and E. coli PqiC with their MCE protein binding partners, PA3213 and PqiB
    Article Snippet: .. Following PCR, DNA fragments were pooled in equimolar amounts to generate the unselected and selected samples for amplicon sequencing with Illumina MiSeq 2 x 250 paired-end sequencing (NEBNext Ultra II Library Prep kit (New England Biologs #E7645) used to generate libraries for sequencing). ..

    Isolation:

    Article Title: Comprehensive genome sequencing associates elevated mitochondrial DNA and homoplasmy with early stage I of pancreatic cancer
    Article Snippet: .. Briefly, cfDNA was isolated using the Qiagen circulating nucleic acid kit (cat# 55114) used in Olou et al., and NGS libraries were prepared for sequencing using New England Bioloab’s (NEB) NEBNext Ultra II DNA library prep for Illumina (cat. #E7645, NEB, Ipswitch, MA, USA). .. Sample libraries were sequenced on the Illumina NextSeq (150 bp paired-end sequencing) to an average read depth of 45.5 million reads.

    Next-Generation Sequencing:

    Article Title: Comprehensive genome sequencing associates elevated mitochondrial DNA and homoplasmy with early stage I of pancreatic cancer
    Article Snippet: .. Briefly, cfDNA was isolated using the Qiagen circulating nucleic acid kit (cat# 55114) used in Olou et al., and NGS libraries were prepared for sequencing using New England Bioloab’s (NEB) NEBNext Ultra II DNA library prep for Illumina (cat. #E7645, NEB, Ipswitch, MA, USA). .. Sample libraries were sequenced on the Illumina NextSeq (150 bp paired-end sequencing) to an average read depth of 45.5 million reads.



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