Polymerase Chain Reaction:Article Title: Multi-omics profiling reveals MAGEL2-driven defects in human corticogenesis shared across Prader-Willi and Schaaf-Yang syndromes
Article Snippet: For the allele identification, gDNA was isolated with the Genomic DNA Purification Kit (STEMCELL Technologies #79020) according to the manufacturer’s instructions . .. SmaI digestion (New England Biolabs #R0141S) with subsequent PCR allowed for amplification of only the methylated maternal allele, resulting in the distinction between paternal and maternal heterozygous MAGEL2 deletions . ..
Article Title: La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathways
Article Snippet: The ΔP6 plasmid was recovered using PCR product and GeneArtTM Gibson Assembly HiFi Master Mix (Thermo Fisher Scientific, # A46624 ) according to the manufacturer’s instructions, mutation was verified by plasmid sequencing. .. AtTR fragments ΔT/PK, ΔP6, and P4/5/6 for Y3H were PCR-amplified (primers specified in ) and ligated into the SmaI site of pIIIA/MS2-2 vector using NEBuilder ® HiFi DNA Assembly Master Mix (New England Biolabs, #E2621S). .. The Gateway cloning system (Invitrogen) was used to introduce DNA fragments encoding desired proteins and protein fragments into pDEST15 or pDEST17 Gateway destination vectors according to the manufacturer’s instructions.
Amplification:Article Title: Multi-omics profiling reveals MAGEL2-driven defects in human corticogenesis shared across Prader-Willi and Schaaf-Yang syndromes
Article Snippet: For the allele identification, gDNA was isolated with the Genomic DNA Purification Kit (STEMCELL Technologies #79020) according to the manufacturer’s instructions . .. SmaI digestion (New England Biolabs #R0141S) with subsequent PCR allowed for amplification of only the methylated maternal allele, resulting in the distinction between paternal and maternal heterozygous MAGEL2 deletions . ..
Methylation:Article Title: Multi-omics profiling reveals MAGEL2-driven defects in human corticogenesis shared across Prader-Willi and Schaaf-Yang syndromes
Article Snippet: For the allele identification, gDNA was isolated with the Genomic DNA Purification Kit (STEMCELL Technologies #79020) according to the manufacturer’s instructions . .. SmaI digestion (New England Biolabs #R0141S) with subsequent PCR allowed for amplification of only the methylated maternal allele, resulting in the distinction between paternal and maternal heterozygous MAGEL2 deletions . ..
Agarose Gel Electrophoresis:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
DNA Extraction:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Transfection:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Plasmid Preparation:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Magnetic Beads:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Protease Inhibitor:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
cDNA Synthesis:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Real-time Polymerase Chain Reaction:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
CCK-8 Assay:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Modification:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Saline:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
In Situ:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Affinity Purification:Article Title: hMPV M2-2 protein modulates host mRNA alternative splicing to favor virus replication
Article Snippet: .. TransStart FastPfu Fly DNA polymerase (TransGen Biotech, Beijing, China; Cat#AP231-22-V4), XhoI (New England Biolabs, Massachusetts, USA; Cat#R0146S), SmaI (New England Biolabs, Massachusetts, USA; CatR0141), rCutSmart buffer (New England Biolabs, Massachusetts, USA; Cat#B6004SVIAL), agarose gel DNA extraction kit (Enhanced) (Tiangen, Beijing, China; Cat#DP219), DH5α competent cells (Weidi Biotechnology, Shanghai, China; Cat#EC1023M), Stbl3 Competent Cells (Weidi Biotechnology, Shanghai, China; Cat#DL1046M), Lipofectamine � RNAiMAX transfection reagent (Thermo Fisher Scientific, Massachusetts, USA; Cat#13778150), plasmid mini kit (Omega Bio-tek, Georgia, USA; Cat#D6904-03), EndoFree midi plasmid kit (Tiangen, Beijing, China; Cat#DP118), Hoechst 33258 (Thermo Fisher Scientific, Massachusetts, USA; Cat#H3569), ANTI-FLAG M2 magnetic beads (SigmaAldrich, Missouri, USA; Cat#M8823-5 mL), 33Flag peptide (GLPBIO, California, USA; Cat#402750-12-3), ProteinSafe� protease inhibitor cocktail (1003) (TransGen Biotech, Beijing, China; Cat#DI111-02), 23 Taq master mix (Vazyme Biotech, Jiangsu, China; Cat#P112-03), TransScript � onestep gDNA removal and cDNA synthesis supermix kit (TransGen Biotech, Beijing, China; Cat#AT311-03), PerfectStart � green qPCR supermix (TransGen Biotech, Beijing, China; Cat#AQ601-04-V2), cell counting kit-8 (Dojindo, Kumamoto Prefecture, Japan; Cat#CK04-500tests), jetOPTIMUS � transfection reagent (Polyplus-transfection, Grand Est, France; Cat#117-07), ampicillin (Sigma-Aldrich, Missouri, USA; Cat#A-9518), Dulbecco’s modified Eagle medium (DMEM) (Gibco, New York, USA; Cat#C11965500BT), Dulbecco’s phosphate -buffered saline (DPBS) (Gibco, New York, USA; Cat#14200075), fetal bovine serum (FBS) (Sigma-Aldrich, Missouri, USA; Cat#F0193), 1% penicillin/ streptomycin antibiotics (Gibco, New York, USA; Cat#15140163), 0.25% trypsin-EDTA (Gibco, New York, USA; Cat#25200114), Duolink � in situ red detection kit (Sigma-Aldrich, Missouri, USA; Cat#DUO92008), affinity-purified donkey anti-rabbit IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92002), affinity-purified donkey anti-mouse IgG (H + L) (Sigma-Aldrich, Missouri, USA; Cat#DUO92004), ProLong gold antifade reagent (Life Technologies, California, USA; Cat#P36930), 4% paraformaldehyde (Biosharp, Anhui, China; Cat#BL539A), BsmBI-v2 (New England Vol. ..
Electrophoresis:Article Title: Comprehensive analysis of antimicrobial resistance and virulence determinants in clinical methicillin-resistant Staphylococcus aureus isolates collected in South Korea.
Article Snippet: The plugs were then washed five times with TE buffer for 20 min each at 55°C using CHEF screened caps (Bio-Rad, Hercules, CA, USA). .. Plug sections were digested with SmaI (New England Biolabs, Ipswich, MA, USA) at 25°C for 4 h. For the reference strain Salmonella enterica ATCC BAA664, plug slices were digested with XbaI (Roche Diagnostics GmbH, Mannheim, Germany) at 37°C for 2.5 h. Digested plug slices were loaded onto 1.2% SeaKem Gold agarose gels (LONZA, Rockland, ME, USA) prepared in 0.5x TBE buffer (Bioneer, Daejeon, Korea) containing 3.75 mM Thiourea (Sigma–Aldrich, St. Louis, MO, USA), and separated using a CHEF Mapper® XA Pulsed Field Electrophoresis System (Bio-Rad, Hercules, CA, USA) under the following conditions: gradient, 6 V/cm; run Time, 18 h; included angle, 120°; initial switch time, 5.16 s; final switch time, 40.17 s; ramping factor, 0. .. Gel images were acquired using the Gel Doc XR+ Gel Documentation system (Bio-Rad, Hercules, CA, USA) and analyzed with BioNumerics version 7.5 (Applied Maths, Sint-Martens-Latem, Belgium).
other:Article Title: Lewis blood group genetic and phenotypic diversity in a Thai population.
Article Snippet: Background: The Lewis blood group (ISBT system 007) is a plasma antigen adsorbed onto the red blood cell surface.. Relatively few studies have examined the genetic characteristics and expression of this blood group or the associated secretor status in different populations.. This study aimed to determine the prevalence of FUT2 and FUT3 gene variants and to investigate the Lewis phenotype and secretor status in a Thai population.
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