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human aml cell lines ht93  (DSMZ)


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    Structured Review

    DSMZ human aml cell lines ht93
    A FASN mRNA levels in <t>AML</t> blasts, CD34 + progenitor cells and granulocytes from healthy donors were quantified by qPCR. All the samples were obtained from the Inselspital, Bern, Switzerland. AML patient cells and granulocytes were isolated using Ficoll gradient density centrifugation. Values are the differences in Ct-values between FASN and the housekeeping genes HMBS and ABL1. MNW * p < 0.05, ** p < 0.0. B Blood spot data bank analysis of FASN expression in AML blasts compared to granulocytes from healthy donors. MNW * p < 0.05, ** p < 0.01. C Western blot analysis of FASN regulation in NB4 and <t>HT93</t> APL cells upon ATRA treatment at different time points (1, 2 and 3 days). Total protein was extracted and submitted to immunoblotting using anti-FASN antibody. Total protein is shown as loading control. The relative protein expressions were normalized to total protein and quantified using ImageJ software (NIH, Bethesda, MD, USA). Data are represented as a mean ( n = 3), Error bars: SD. D Evaluation of FASN transcript levels upon ATRA treatment was done by qPCR. Values were normalized to the HMBS housekeeping gene. Results of at least three independent experiments are shown as n-fold regulation compared with non-treated cells.
    Human Aml Cell Lines Ht93, supplied by DSMZ, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/statistical+analysis+software%2C+program+version+9%2E3/HT-93/pmc08329134-35-1-19
    Average 88 stars, based on 4 article reviews
    human aml cell lines ht93 - by Bioz Stars, 2026-10
    88/100 stars

    Images

    1) Product Images from "Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy"

    Article Title: Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy

    Journal: Cell Death and Differentiation

    doi: 10.1038/s41418-021-00768-1

    A FASN mRNA levels in AML blasts, CD34 + progenitor cells and granulocytes from healthy donors were quantified by qPCR. All the samples were obtained from the Inselspital, Bern, Switzerland. AML patient cells and granulocytes were isolated using Ficoll gradient density centrifugation. Values are the differences in Ct-values between FASN and the housekeeping genes HMBS and ABL1. MNW * p < 0.05, ** p < 0.0. B Blood spot data bank analysis of FASN expression in AML blasts compared to granulocytes from healthy donors. MNW * p < 0.05, ** p < 0.01. C Western blot analysis of FASN regulation in NB4 and HT93 APL cells upon ATRA treatment at different time points (1, 2 and 3 days). Total protein was extracted and submitted to immunoblotting using anti-FASN antibody. Total protein is shown as loading control. The relative protein expressions were normalized to total protein and quantified using ImageJ software (NIH, Bethesda, MD, USA). Data are represented as a mean ( n = 3), Error bars: SD. D Evaluation of FASN transcript levels upon ATRA treatment was done by qPCR. Values were normalized to the HMBS housekeeping gene. Results of at least three independent experiments are shown as n-fold regulation compared with non-treated cells.
    Figure Legend Snippet: A FASN mRNA levels in AML blasts, CD34 + progenitor cells and granulocytes from healthy donors were quantified by qPCR. All the samples were obtained from the Inselspital, Bern, Switzerland. AML patient cells and granulocytes were isolated using Ficoll gradient density centrifugation. Values are the differences in Ct-values between FASN and the housekeeping genes HMBS and ABL1. MNW * p < 0.05, ** p < 0.0. B Blood spot data bank analysis of FASN expression in AML blasts compared to granulocytes from healthy donors. MNW * p < 0.05, ** p < 0.01. C Western blot analysis of FASN regulation in NB4 and HT93 APL cells upon ATRA treatment at different time points (1, 2 and 3 days). Total protein was extracted and submitted to immunoblotting using anti-FASN antibody. Total protein is shown as loading control. The relative protein expressions were normalized to total protein and quantified using ImageJ software (NIH, Bethesda, MD, USA). Data are represented as a mean ( n = 3), Error bars: SD. D Evaluation of FASN transcript levels upon ATRA treatment was done by qPCR. Values were normalized to the HMBS housekeeping gene. Results of at least three independent experiments are shown as n-fold regulation compared with non-treated cells.

    Techniques Used: Isolation, Centrifugation, Clinical Proteomics, Expressing, Western Blot, Control, Software

    Related Articles

    Isolation:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Centrifugation:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Clinical Proteomics:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Expressing:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Western Blot:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Control:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Software:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Translocation Assay:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Immunofluorescence:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Incubation:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Activity Assay:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Construct:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Transduction:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Knockdown:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Generated:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    MANN-WHITNEY:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Selection:

    Article Title: Distinct TP73-DAPK2-ATG5 pathway involvement in ATO-mediated cell death versus ATRA-mediated autophagy responses in APL.
    Article Snippet: Cell lines and culture conditions The human APL cell lines, HT93 and NB4, were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).



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