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Santa Cruz Biotechnology erk
Suppressor of cytokine signaling 1 attenuates invasive growth of orthotopic Hepa cell tumors. A: Hepa-V and Hepa-SOCS1 cells were injected via intravenous route into NOD. scid.gamma (NSG) mice and the livers were examined macroscopically after 20 d. Representative images (left) and quantification of the liver tumor nodules (right) from four mice per group are shown; B-D: Hepa-vector and Hepa-SOCS1 were injected via intrasplenic route into NSG mice (1 × 10 6 cells per mouse; n = 6 per group from two separate experiments) and the mice were splenectomized. After 20 d, the liver tissues were examined macroscopically for tumor nodules. The dorsal and ventral views of representative livers (B, left) and H and E-stained sections of a representative liver for each group (B, right) are shown; C: For each group, the areas of thirty random tumor nodules from three different mice were measured digitally using the NDP software and compared by Student’s paired t test; D: Western blot evaluation <t>of</t> <t>STAT3</t> and <t>ERK</t> phosphorylation in the tumors from 2 mice per group; E: Western blot evaluation of MET expression and its quantification in the tumors from 3 mice per group. SOCS1: Suppressor of cytokine signaling 1.
Erk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1"

Article Title: Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1

Journal: World Journal of Gastroenterology

doi: 10.3748/wjg.v23.i36.6639

Suppressor of cytokine signaling 1 attenuates invasive growth of orthotopic Hepa cell tumors. A: Hepa-V and Hepa-SOCS1 cells were injected via intravenous route into NOD. scid.gamma (NSG) mice and the livers were examined macroscopically after 20 d. Representative images (left) and quantification of the liver tumor nodules (right) from four mice per group are shown; B-D: Hepa-vector and Hepa-SOCS1 were injected via intrasplenic route into NSG mice (1 × 10 6 cells per mouse; n = 6 per group from two separate experiments) and the mice were splenectomized. After 20 d, the liver tissues were examined macroscopically for tumor nodules. The dorsal and ventral views of representative livers (B, left) and H and E-stained sections of a representative liver for each group (B, right) are shown; C: For each group, the areas of thirty random tumor nodules from three different mice were measured digitally using the NDP software and compared by Student’s paired t test; D: Western blot evaluation of STAT3 and ERK phosphorylation in the tumors from 2 mice per group; E: Western blot evaluation of MET expression and its quantification in the tumors from 3 mice per group. SOCS1: Suppressor of cytokine signaling 1.
Figure Legend Snippet: Suppressor of cytokine signaling 1 attenuates invasive growth of orthotopic Hepa cell tumors. A: Hepa-V and Hepa-SOCS1 cells were injected via intravenous route into NOD. scid.gamma (NSG) mice and the livers were examined macroscopically after 20 d. Representative images (left) and quantification of the liver tumor nodules (right) from four mice per group are shown; B-D: Hepa-vector and Hepa-SOCS1 were injected via intrasplenic route into NSG mice (1 × 10 6 cells per mouse; n = 6 per group from two separate experiments) and the mice were splenectomized. After 20 d, the liver tissues were examined macroscopically for tumor nodules. The dorsal and ventral views of representative livers (B, left) and H and E-stained sections of a representative liver for each group (B, right) are shown; C: For each group, the areas of thirty random tumor nodules from three different mice were measured digitally using the NDP software and compared by Student’s paired t test; D: Western blot evaluation of STAT3 and ERK phosphorylation in the tumors from 2 mice per group; E: Western blot evaluation of MET expression and its quantification in the tumors from 3 mice per group. SOCS1: Suppressor of cytokine signaling 1.

Techniques Used: Injection, Plasmid Preparation, Staining, Software, Western Blot, Phospho-proteomics, Expressing

Related Articles

other:

Article Title: The portrait of liver cancer is shaped by mitochondrial genetics
Article Snippet: Anti-ERK , Santacruz Biotechnology , Cat No.: sc-153; RRID: AB_2141293.

Membrane:

Article Title: Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression.
Article Snippet: Briefly, protein concentration was measured by the Bradford assay (#5000006; Bio-Rad, USA), and 20 μg of proteins from each sample were separated by SDS-PAGE and transferred onto PVDF membranes by electrophoretic transfer. .. The membrane was blocked with 5% non-fat skim milk in TBS-Tween and incubated with antibodies against NELL2 (1:1,000, #sc-390137; Santa Cruz Biotechnology, USA), pERK (1:1,000, #9101; Cell Signaling Technology, USA), ERK (1:1,000, #sc-153; Santa Cruz Biotechnology), or cFos (1:1,000, #sc-7202; Santa Cruz Biotechnology). .. The membrane was incubated with HRP-conjugated mouse secondary antibody (1:3,000, #7076; Cell Signaling Technology) or HRP-conjugated rabbit secondary antibody (1:3,000, #7074; Cell Signaling Technology), and the immunoreactive signals were detected by chemiluminescent detection reagent (#34095, Thermo Fisher Scientific, USA).

Incubation:

Article Title: Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression.
Article Snippet: Briefly, protein concentration was measured by the Bradford assay (#5000006; Bio-Rad, USA), and 20 μg of proteins from each sample were separated by SDS-PAGE and transferred onto PVDF membranes by electrophoretic transfer. .. The membrane was blocked with 5% non-fat skim milk in TBS-Tween and incubated with antibodies against NELL2 (1:1,000, #sc-390137; Santa Cruz Biotechnology, USA), pERK (1:1,000, #9101; Cell Signaling Technology, USA), ERK (1:1,000, #sc-153; Santa Cruz Biotechnology), or cFos (1:1,000, #sc-7202; Santa Cruz Biotechnology). .. The membrane was incubated with HRP-conjugated mouse secondary antibody (1:3,000, #7076; Cell Signaling Technology) or HRP-conjugated rabbit secondary antibody (1:3,000, #7074; Cell Signaling Technology), and the immunoreactive signals were detected by chemiluminescent detection reagent (#34095, Thermo Fisher Scientific, USA).



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