cmt 93 cmt 93 (ATCC)

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ATCC cmt 93 cmt 93

RIS exposure attenuates proapoptotic signaling molecules in vivo. A, allograft assay with <t>CMT-93</t> cells pre-exposed to DON. CMT-93 C57BL/6 mouse colon cancer cells pre-exposed to 500 ng/ml DON for 24 h were dissociated into single cells with trypsin, and 5 × 106 cells resuspended with 200 μl of PBS were injected subcutaneously into 14-week-old male C57BL/6 mice. Seven days later, 100 mg/ml 5-FU was intraperitoneally injected, and tumors were excised surgically 24 h later. The tumor volume was calculated as tumor volume = π/6 × major diameter (W) × minor diameter (L)2, presented by vertical scatter plot, and statistically analyzed by unpaired two-tailed t test (bottom panel). The difference in tumor volume was statistically significant (**, p = 0.0022). Con, control. B, a histological section of allograft tumors was analyzed by immunohistochemistry with MIC-1, EGR-1, and ATF3. 3,3′-diaminobenzidine intensity versus hematoxylin was quantitatively assessed by HistoQuest software and statistically analyzed by unpaired two-tailed t test (bottom panel). **, p < 0.01; ***, p < 0.001.

Cmt 93 Cmt 93, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/statistical+analysis+software%2C+program+version+9%2E3/CMT-93/pmc04858968-129-2-15
Average 95 stars, based on 263 article reviews

cmt 93 cmt 93 - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "Acquisition of Chemoresistance and Other Malignancy-related Features of Colorectal Cancer Cells Are Incremented by Ribosome-inactivating Stress * "

Article Title: Acquisition of Chemoresistance and Other Malignancy-related Features of Colorectal Cancer Cells Are Incremented by Ribosome-inactivating Stress *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M115.696609

RIS exposure attenuates proapoptotic signaling molecules in vivo. A, allograft assay with CMT-93 cells pre-exposed to DON. CMT-93 C57BL/6 mouse colon cancer cells pre-exposed to 500 ng/ml DON for 24 h were dissociated into single cells with trypsin, and 5 × 106 cells resuspended with 200 μl of PBS were injected subcutaneously into 14-week-old male C57BL/6 mice. Seven days later, 100 mg/ml 5-FU was intraperitoneally injected, and tumors were excised surgically 24 h later. The tumor volume was calculated as tumor volume = π/6 × major diameter (W) × minor diameter (L)2, presented by vertical scatter plot, and statistically analyzed by unpaired two-tailed t test (bottom panel). The difference in tumor volume was statistically significant (**, p = 0.0022). Con, control. B, a histological section of allograft tumors was analyzed by immunohistochemistry with MIC-1, EGR-1, and ATF3. 3,3′-diaminobenzidine intensity versus hematoxylin was quantitatively assessed by HistoQuest software and statistically analyzed by unpaired two-tailed t test (bottom panel). **, p < 0.01; ***, p < 0.001.

Figure Legend Snippet: RIS exposure attenuates proapoptotic signaling molecules in vivo. A, allograft assay with CMT-93 cells pre-exposed to DON. CMT-93 C57BL/6 mouse colon cancer cells pre-exposed to 500 ng/ml DON for 24 h were dissociated into single cells with trypsin, and 5 × 106 cells resuspended with 200 μl of PBS were injected subcutaneously into 14-week-old male C57BL/6 mice. Seven days later, 100 mg/ml 5-FU was intraperitoneally injected, and tumors were excised surgically 24 h later. The tumor volume was calculated as tumor volume = π/6 × major diameter (W) × minor diameter (L)2, presented by vertical scatter plot, and statistically analyzed by unpaired two-tailed t test (bottom panel). The difference in tumor volume was statistically significant (**, p = 0.0022). Con, control. B, a histological section of allograft tumors was analyzed by immunohistochemistry with MIC-1, EGR-1, and ATF3. 3,3′-diaminobenzidine intensity versus hematoxylin was quantitatively assessed by HistoQuest software and statistically analyzed by unpaired two-tailed t test (bottom panel). **, p < 0.01; ***, p < 0.001.

Techniques Used: In Vivo, Injection, Two Tailed Test, Immunohistochemistry, Software

Modification:

Article Title: Purification and Characterization of His-Tagged Recombinant Bacteroides fragilis Toxin-2 Variants In Vitro and In Vivo.
Article Snippet: .. Human colonic epithelial cell line HT29/c1 (#HTB-38, ATCC, Manassas, VA, USA; passage 130) and mouse rectal epithelial cell line CMT93 (#CCL-223, ATCC, VA, USA; passage 75) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, #MD10000A, Bandio Bio Science, Pocheon, Republic of Korea) supplemented with 5% fetal bovine serum (FBS, #35-015-CV, Corning, Palo Alto, CA, USA), 20 mM HEPES (#15630-080, Gibco, Miami, FL, USA), without penicillin-streptomycin [51,52]. ..

Article Title: Immunogenicity and plasmid delivery pathways of non-invasive Lactococcus lactis -vectored mucosal DNA vaccination
Article Snippet: .. The murine colonic epithelial cell line CMT-93 (ATCC) was cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS at 37°C in 5% CO 2 . .. The human retinal pigment epithelial cell line ARPE-19 (ATCC) was cultured in high-glucose DMEM/Ham’s F-12 supplemented with 10% FBS at 37°C in 5% CO 2 .

Article Title: Purification and Characterization of His-Tagged Recombinant Bacteroides fragilis Toxin-2 Variants In Vitro and In Vivo
Article Snippet: .. Human colonic epithelial cell line HT29/c1 (#HTB-38, ATCC, Manassas, VA, USA; passage 130) and mouse rectal epithelial cell line CMT93 (#CCL-223, ATCC, VA, USA; passage 75) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, #MD10000A, Bandio Bio Science, Pocheon, Republic of Korea) supplemented with 5% fetal bovine serum (FBS, #35-015-CV, Corning, Palo Alto, CA, USA), 20 mM HEPES (#15630-080, Gibco, Miami, FL, USA), without penicillin-streptomycin [ , ]. ..

Cell Culture:

Derivative Assay:

Article Title: Metformin enhances TRAIL-mediated antitumor activity of liver natural killer cells in mice.
Article Snippet: .. The mouse epithelial rectal carcinoma cell line CMT-93 (derived from C57BL mice) was purchased from the American Type Culture Collection (Manassas, VA, USA). ..

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