mnase digestions (New England Biolabs)
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mnase digestions
Mnase Digestions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stata+12+0+software+programme/Micrococcal+Nuclease/pmc04737182-37-4-26
Average 99 stars, based on 2293 article reviews
Mnase Digestions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stata+12+0+software+programme/Micrococcal+Nuclease/pmc04737182-37-4-26
Average 99 stars, based on 2293 article reviews
mnase digestions - by Bioz Stars,
2026-11
99/100 stars
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Virus:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Recombinant:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Article Title: Proximity of pre-mRNA 3' end processing and transcription termination is associated with enhanced gene expression. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models 1CT (H. sapiens) Shazia Irshad’s Lab n/a HCT116 (H. sapiens) The Maria Skłodowska–Curie Greater Poland Cancer Centre n/a SW480 (H. sapiens) Shazia Irshad’s Lab n/a SW620 (H. sapiens) Shazia Irshad’s Lab n/a © The Author(s) The EMBO Journal 13 Reagent/resource Reference or source Identifier or catalog number HCT116 CPSF73-AID (H. sapiens) Steve West’s Lab n/a T84 (H. sapiens) Shazia Irshad’s Lab n/a HeLa (H. sapiens) Nick Proudfoot’s Lab n/a Panc1 Argyris Papantonis’s Lab n/a MiaPaCa2 Argyris Papantonis’s Lab n/a BxPC3 Argyris Papantonis’s Lab n/a Recombinant DNA n/a Antibodies Rabbit anti-PCF11 Abcam Cat# ab134391; RRID: AB_2783786 Rabbit anti-CPSF73 Abcam Cat# ab72295, RRID:AB_1268249 Rabbit anti-XRN2 Thermo Fisher Scientific Cat# A301-103A, RRID:AB_2218876 Mouse monoclonal anti-RNAPII total Stasevich et al. 2014 Cat# CMA601; RRID: AB_2827956 Rat monoclonal antiRNAPII T4ph Active Motif Cat# 61361, RRID:AB_2750848 Rabbit monoclonal anti-RNAPII CTD Ser2 Abcam Cat# ab193468; RRID:AB_2905557 Mouse anti-PCNA Santa Cruz Biotechnology Cat# sc-56, RRID:AB_628110 Goat anti-rabbit IgG —Peroxidase MERCK Cat# A0545; RRID:AB_257896 Goat anti-rabbit IgG – IRDye 800CW LI-COR Cat# 926-32211; RRID:AB_621843 Oligonucleotides and other sequence-based reagents siLUC MERCK Sequence (5′-3′) Sense: GAUUAUGUCCGGUUAU GUAUU Antisense: [phos] UACAUAACCGGA CA UAAUCUU siPCF11 (human) ONTARGETplus SMARTpool Dharmacon Cat# L-015381-01 siCPSF73 (human) ON-TARGETplus SMARTpool Dharmacon Cat# L-006365-01 Chemicals, enzymes and other reagents JTE-607 MERCK Cat# SML2833 Human EGF Thermo Fisher Scientific Cat# PHG6045 Hydrocortisone MERCK Cat# H0888-1G Apo-Transferrin MERCK Cat# T2036-100MG Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150 Reagent/resource Reference or source Identifier or catalog number 7.5% and 4–15% Mini-PROTEAN TGX Precast Protein Gels Bio-Rad Cat# 4561026, 4561086 Novex 6% TBE gel, 12-well Thermo Fisher Scientific Cat# EC62652BOX Novex 6% TBE-Urea (TBU) gel, 12-well Thermo Fisher Scientific Cat# EC68652BOX T4 polynucleotide kinase (PNK), 3’ phosphatase minus NEB Cat# M0236S TRI Reagent Solution Thermo Fisher Scientific Cat# AM9738 Protease Inhibitor:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Article Title: Developmental rewiring of EHMT1 function during human cortical neurogenesis Article Snippet: .. Briefly, flash-frozen cell pellets were resuspended in Sigma EZ Nuclei Isolation Lysis Buffer (NUC101) containing 1× protease inhibitor cocktail (Roche 04693132001) and 1 mM PMSF (TF 36978), and then digested with Cloning:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Gel Extraction:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Purification:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Plasmid Preparation:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 DNA Extraction:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Staining:Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Lysis:Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma. Article Snippet: Nuclei were then crosslinked with 3 mM ethylene glycolbis (succinimidyl succinate) (Thermo Fisher Scientific) at room temperature for 40 min, terminated by 0.4 M glycine treatment and a PBS wash containing 0.05% BSA. .. After cell lysis, chromatin was fragmented using Article Title: Developmental rewiring of EHMT1 function during human cortical neurogenesis Article Snippet: .. Briefly, flash-frozen cell pellets were resuspended in Sigma EZ Nuclei Isolation Lysis Buffer (NUC101) containing 1× protease inhibitor cocktail (Roche 04693132001) and 1 mM PMSF (TF 36978), and then digested with Titration:Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma. Article Snippet: Nuclei were then crosslinked with 3 mM ethylene glycolbis (succinimidyl succinate) (Thermo Fisher Scientific) at room temperature for 40 min, terminated by 0.4 M glycine treatment and a PBS wash containing 0.05% BSA. .. After cell lysis, chromatin was fragmented using Inactivation:Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma. Article Snippet: Nuclei were then crosslinked with 3 mM ethylene glycolbis (succinimidyl succinate) (Thermo Fisher Scientific) at room temperature for 40 min, terminated by 0.4 M glycine treatment and a PBS wash containing 0.05% BSA. .. After cell lysis, chromatin was fragmented using De-Phosphorylation Assay:Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma. Article Snippet: Nuclei were then crosslinked with 3 mM ethylene glycolbis (succinimidyl succinate) (Thermo Fisher Scientific) at room temperature for 40 min, terminated by 0.4 M glycine treatment and a PBS wash containing 0.05% BSA. .. After cell lysis, chromatin was fragmented using Phospho-proteomics:Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma. Article Snippet: Nuclei were then crosslinked with 3 mM ethylene glycolbis (succinimidyl succinate) (Thermo Fisher Scientific) at room temperature for 40 min, terminated by 0.4 M glycine treatment and a PBS wash containing 0.05% BSA. .. After cell lysis, chromatin was fragmented using Analysis:Article Title: Anisomycin-induced ribosome collisions follow a dose-dependent positional rule, distinct from the UV-induced collision landscape Article Snippet: .. Nuclease treatment prior to polysome analysis was conducted by supplementing the appropriate volume of lysate corresponding to 180 μg of protein (ca. 120-150 μL) lysate with 1 mM CaCl2, 3 μL (6000 U) Incubation:Article Title: Anisomycin-induced ribosome collisions follow a dose-dependent positional rule, distinct from the UV-induced collision landscape Article Snippet: .. Nuclease treatment prior to polysome analysis was conducted by supplementing the appropriate volume of lysate corresponding to 180 μg of protein (ca. 120-150 μL) lysate with 1 mM CaCl2, 3 μL (6000 U) Isolation:Article Title: Developmental rewiring of EHMT1 function during human cortical neurogenesis Article Snippet: .. Briefly, flash-frozen cell pellets were resuspended in Sigma EZ Nuclei Isolation Lysis Buffer (NUC101) containing 1× protease inhibitor cocktail (Roche 04693132001) and 1 mM PMSF (TF 36978), and then digested with Sequencing:Article Title: Proximity of pre-mRNA 3' end processing and transcription termination is associated with enhanced gene expression. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models 1CT (H. sapiens) Shazia Irshad’s Lab n/a HCT116 (H. sapiens) The Maria Skłodowska–Curie Greater Poland Cancer Centre n/a SW480 (H. sapiens) Shazia Irshad’s Lab n/a SW620 (H. sapiens) Shazia Irshad’s Lab n/a © The Author(s) The EMBO Journal 13 Reagent/resource Reference or source Identifier or catalog number HCT116 CPSF73-AID (H. sapiens) Steve West’s Lab n/a T84 (H. sapiens) Shazia Irshad’s Lab n/a HeLa (H. sapiens) Nick Proudfoot’s Lab n/a Panc1 Argyris Papantonis’s Lab n/a MiaPaCa2 Argyris Papantonis’s Lab n/a BxPC3 Argyris Papantonis’s Lab n/a Recombinant DNA n/a Antibodies Rabbit anti-PCF11 Abcam Cat# ab134391; RRID: AB_2783786 Rabbit anti-CPSF73 Abcam Cat# ab72295, RRID:AB_1268249 Rabbit anti-XRN2 Thermo Fisher Scientific Cat# A301-103A, RRID:AB_2218876 Mouse monoclonal anti-RNAPII total Stasevich et al. 2014 Cat# CMA601; RRID: AB_2827956 Rat monoclonal antiRNAPII T4ph Active Motif Cat# 61361, RRID:AB_2750848 Rabbit monoclonal anti-RNAPII CTD Ser2 Abcam Cat# ab193468; RRID:AB_2905557 Mouse anti-PCNA Santa Cruz Biotechnology Cat# sc-56, RRID:AB_628110 Goat anti-rabbit IgG —Peroxidase MERCK Cat# A0545; RRID:AB_257896 Goat anti-rabbit IgG – IRDye 800CW LI-COR Cat# 926-32211; RRID:AB_621843 Oligonucleotides and other sequence-based reagents siLUC MERCK Sequence (5′-3′) Sense: GAUUAUGUCCGGUUAU GUAUU Antisense: [phos] UACAUAACCGGA CA UAAUCUU siPCF11 (human) ONTARGETplus SMARTpool Dharmacon Cat# L-015381-01 siCPSF73 (human) ON-TARGETplus SMARTpool Dharmacon Cat# L-006365-01 Chemicals, enzymes and other reagents JTE-607 MERCK Cat# SML2833 Human EGF Thermo Fisher Scientific Cat# PHG6045 Hydrocortisone MERCK Cat# H0888-1G Apo-Transferrin MERCK Cat# T2036-100MG Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150 Reagent/resource Reference or source Identifier or catalog number 7.5% and 4–15% Mini-PROTEAN TGX Precast Protein Gels Bio-Rad Cat# 4561026, 4561086 Novex 6% TBE gel, 12-well Thermo Fisher Scientific Cat# EC62652BOX Novex 6% TBE-Urea (TBU) gel, 12-well Thermo Fisher Scientific Cat# EC68652BOX T4 polynucleotide kinase (PNK), 3’ phosphatase minus NEB Cat# M0236S TRI Reagent Solution Thermo Fisher Scientific Cat# AM9738 Transfection:Article Title: Proximity of pre-mRNA 3' end processing and transcription termination is associated with enhanced gene expression. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models 1CT (H. sapiens) Shazia Irshad’s Lab n/a HCT116 (H. sapiens) The Maria Skłodowska–Curie Greater Poland Cancer Centre n/a SW480 (H. sapiens) Shazia Irshad’s Lab n/a SW620 (H. sapiens) Shazia Irshad’s Lab n/a © The Author(s) The EMBO Journal 13 Reagent/resource Reference or source Identifier or catalog number HCT116 CPSF73-AID (H. sapiens) Steve West’s Lab n/a T84 (H. sapiens) Shazia Irshad’s Lab n/a HeLa (H. sapiens) Nick Proudfoot’s Lab n/a Panc1 Argyris Papantonis’s Lab n/a MiaPaCa2 Argyris Papantonis’s Lab n/a BxPC3 Argyris Papantonis’s Lab n/a Recombinant DNA n/a Antibodies Rabbit anti-PCF11 Abcam Cat# ab134391; RRID: AB_2783786 Rabbit anti-CPSF73 Abcam Cat# ab72295, RRID:AB_1268249 Rabbit anti-XRN2 Thermo Fisher Scientific Cat# A301-103A, RRID:AB_2218876 Mouse monoclonal anti-RNAPII total Stasevich et al. 2014 Cat# CMA601; RRID: AB_2827956 Rat monoclonal antiRNAPII T4ph Active Motif Cat# 61361, RRID:AB_2750848 Rabbit monoclonal anti-RNAPII CTD Ser2 Abcam Cat# ab193468; RRID:AB_2905557 Mouse anti-PCNA Santa Cruz Biotechnology Cat# sc-56, RRID:AB_628110 Goat anti-rabbit IgG —Peroxidase MERCK Cat# A0545; RRID:AB_257896 Goat anti-rabbit IgG – IRDye 800CW LI-COR Cat# 926-32211; RRID:AB_621843 Oligonucleotides and other sequence-based reagents siLUC MERCK Sequence (5′-3′) Sense: GAUUAUGUCCGGUUAU GUAUU Antisense: [phos] UACAUAACCGGA CA UAAUCUU siPCF11 (human) ONTARGETplus SMARTpool Dharmacon Cat# L-015381-01 siCPSF73 (human) ON-TARGETplus SMARTpool Dharmacon Cat# L-006365-01 Chemicals, enzymes and other reagents JTE-607 MERCK Cat# SML2833 Human EGF Thermo Fisher Scientific Cat# PHG6045 Hydrocortisone MERCK Cat# H0888-1G Apo-Transferrin MERCK Cat# T2036-100MG Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150 Reagent/resource Reference or source Identifier or catalog number 7.5% and 4–15% Mini-PROTEAN TGX Precast Protein Gels Bio-Rad Cat# 4561026, 4561086 Novex 6% TBE gel, 12-well Thermo Fisher Scientific Cat# EC62652BOX Novex 6% TBE-Urea (TBU) gel, 12-well Thermo Fisher Scientific Cat# EC68652BOX T4 polynucleotide kinase (PNK), 3’ phosphatase minus NEB Cat# M0236S TRI Reagent Solution Thermo Fisher Scientific Cat# AM9738 Software:Article Title: Proximity of pre-mRNA 3' end processing and transcription termination is associated with enhanced gene expression. Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models 1CT (H. sapiens) Shazia Irshad’s Lab n/a HCT116 (H. sapiens) The Maria Skłodowska–Curie Greater Poland Cancer Centre n/a SW480 (H. sapiens) Shazia Irshad’s Lab n/a SW620 (H. sapiens) Shazia Irshad’s Lab n/a © The Author(s) The EMBO Journal 13 Reagent/resource Reference or source Identifier or catalog number HCT116 CPSF73-AID (H. sapiens) Steve West’s Lab n/a T84 (H. sapiens) Shazia Irshad’s Lab n/a HeLa (H. sapiens) Nick Proudfoot’s Lab n/a Panc1 Argyris Papantonis’s Lab n/a MiaPaCa2 Argyris Papantonis’s Lab n/a BxPC3 Argyris Papantonis’s Lab n/a Recombinant DNA n/a Antibodies Rabbit anti-PCF11 Abcam Cat# ab134391; RRID: AB_2783786 Rabbit anti-CPSF73 Abcam Cat# ab72295, RRID:AB_1268249 Rabbit anti-XRN2 Thermo Fisher Scientific Cat# A301-103A, RRID:AB_2218876 Mouse monoclonal anti-RNAPII total Stasevich et al. 2014 Cat# CMA601; RRID: AB_2827956 Rat monoclonal antiRNAPII T4ph Active Motif Cat# 61361, RRID:AB_2750848 Rabbit monoclonal anti-RNAPII CTD Ser2 Abcam Cat# ab193468; RRID:AB_2905557 Mouse anti-PCNA Santa Cruz Biotechnology Cat# sc-56, RRID:AB_628110 Goat anti-rabbit IgG —Peroxidase MERCK Cat# A0545; RRID:AB_257896 Goat anti-rabbit IgG – IRDye 800CW LI-COR Cat# 926-32211; RRID:AB_621843 Oligonucleotides and other sequence-based reagents siLUC MERCK Sequence (5′-3′) Sense: GAUUAUGUCCGGUUAU GUAUU Antisense: [phos] UACAUAACCGGA CA UAAUCUU siPCF11 (human) ONTARGETplus SMARTpool Dharmacon Cat# L-015381-01 siCPSF73 (human) ON-TARGETplus SMARTpool Dharmacon Cat# L-006365-01 Chemicals, enzymes and other reagents JTE-607 MERCK Cat# SML2833 Human EGF Thermo Fisher Scientific Cat# PHG6045 Hydrocortisone MERCK Cat# H0888-1G Apo-Transferrin MERCK Cat# T2036-100MG Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150 Reagent/resource Reference or source Identifier or catalog number 7.5% and 4–15% Mini-PROTEAN TGX Precast Protein Gels Bio-Rad Cat# 4561026, 4561086 Novex 6% TBE gel, 12-well Thermo Fisher Scientific Cat# EC62652BOX Novex 6% TBE-Urea (TBU) gel, 12-well Thermo Fisher Scientific Cat# EC68652BOX T4 polynucleotide kinase (PNK), 3’ phosphatase minus NEB Cat# M0236S TRI Reagent Solution Thermo Fisher Scientific Cat# AM9738 |