Western Blot:Article Title: Mechanism of IL-4 mediated RPL19 promoting malignant progression in HER2 positive breast cancer.
Article Snippet: .. Antibody Source Catalog number Applications Rabbit anti-RPL19 Proteintech 14701-1-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Mouse anti-IL4 Proteintech 66142-1-Ig WB,IHC,IF-P,FC (Intra), ELISA Rabbit anti-JUN Proteintech 24909-1-AP WB, IHC, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT6 Proteintech 51073-1-AP WB, IHC, ELISA Rabbit anti-p-STAT6 Affiniyt AF3301 WB,IHC,IF/ICC Rabbit anti-AKT Proteintech 10176-2-AP WB, IHC, IF-P, IF-Fro, IP, ELISA Rabbit anti-p-AKT Proteintech 66444-1-Ig WB, IHC, IF/ICC, FC (Intra), ELISA AR TIC LE IN PR ES S Rabbit anti-MAPK Proteintech 14064-1-AP WB, IHC, ELISA Rabbit anti-p-MAPK Proteintech 80024-1-RR WB, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT3 Proteintech 10253-2-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Rabbit anti-p-STAT3 Proteintech 28945-1-AP WB, ELISA Rabbit anti-IL1-β Proteintech 26048-1-AP WB, ELISA Rabbit anti-TNF-α Proteintech 17590-1-AP WB, ELISA Mouse anti-IL10 Proteintech 60269-1-Ig WB,IHC,IF/ICC, ELISA Rabbit anti-ARG1 Proteintech 16001-1-AP WB, IHC, IF/ICC, IF-P, IP, ELISA Mouse anti-GAPDH Proteintech 60004-1-Ig WB,IF/ICC,FC (Intra), IP, ELISA Rabbit anti-β-ACTIN ABclonal AC026 WB,IHC-P,ELISA Hematoxylin and eosin (H&E) staining Hematoxylin and eosin was conducted following the procedure previously outlined[81]. .. Cell culture and transfection The AU565, UACC812, and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/high glucose (Meilune, Dalian, China) or RPMI-1640 (Meilune, Dalian, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA) and 1% penicillin–streptomycin (Meilune, Dalian, China).
Immunohistochemistry:Article Title: Mechanism of IL-4 mediated RPL19 promoting malignant progression in HER2 positive breast cancer.
Article Snippet: .. Antibody Source Catalog number Applications Rabbit anti-RPL19 Proteintech 14701-1-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Mouse anti-IL4 Proteintech 66142-1-Ig WB,IHC,IF-P,FC (Intra), ELISA Rabbit anti-JUN Proteintech 24909-1-AP WB, IHC, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT6 Proteintech 51073-1-AP WB, IHC, ELISA Rabbit anti-p-STAT6 Affiniyt AF3301 WB,IHC,IF/ICC Rabbit anti-AKT Proteintech 10176-2-AP WB, IHC, IF-P, IF-Fro, IP, ELISA Rabbit anti-p-AKT Proteintech 66444-1-Ig WB, IHC, IF/ICC, FC (Intra), ELISA AR TIC LE IN PR ES S Rabbit anti-MAPK Proteintech 14064-1-AP WB, IHC, ELISA Rabbit anti-p-MAPK Proteintech 80024-1-RR WB, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT3 Proteintech 10253-2-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Rabbit anti-p-STAT3 Proteintech 28945-1-AP WB, ELISA Rabbit anti-IL1-β Proteintech 26048-1-AP WB, ELISA Rabbit anti-TNF-α Proteintech 17590-1-AP WB, ELISA Mouse anti-IL10 Proteintech 60269-1-Ig WB,IHC,IF/ICC, ELISA Rabbit anti-ARG1 Proteintech 16001-1-AP WB, IHC, IF/ICC, IF-P, IP, ELISA Mouse anti-GAPDH Proteintech 60004-1-Ig WB,IF/ICC,FC (Intra), IP, ELISA Rabbit anti-β-ACTIN ABclonal AC026 WB,IHC-P,ELISA Hematoxylin and eosin (H&E) staining Hematoxylin and eosin was conducted following the procedure previously outlined[81]. .. Cell culture and transfection The AU565, UACC812, and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/high glucose (Meilune, Dalian, China) or RPMI-1640 (Meilune, Dalian, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA) and 1% penicillin–streptomycin (Meilune, Dalian, China).
Enzyme-linked Immunosorbent Assay:Article Title: Mechanism of IL-4 mediated RPL19 promoting malignant progression in HER2 positive breast cancer.
Article Snippet: .. Antibody Source Catalog number Applications Rabbit anti-RPL19 Proteintech 14701-1-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Mouse anti-IL4 Proteintech 66142-1-Ig WB,IHC,IF-P,FC (Intra), ELISA Rabbit anti-JUN Proteintech 24909-1-AP WB, IHC, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT6 Proteintech 51073-1-AP WB, IHC, ELISA Rabbit anti-p-STAT6 Affiniyt AF3301 WB,IHC,IF/ICC Rabbit anti-AKT Proteintech 10176-2-AP WB, IHC, IF-P, IF-Fro, IP, ELISA Rabbit anti-p-AKT Proteintech 66444-1-Ig WB, IHC, IF/ICC, FC (Intra), ELISA AR TIC LE IN PR ES S Rabbit anti-MAPK Proteintech 14064-1-AP WB, IHC, ELISA Rabbit anti-p-MAPK Proteintech 80024-1-RR WB, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT3 Proteintech 10253-2-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Rabbit anti-p-STAT3 Proteintech 28945-1-AP WB, ELISA Rabbit anti-IL1-β Proteintech 26048-1-AP WB, ELISA Rabbit anti-TNF-α Proteintech 17590-1-AP WB, ELISA Mouse anti-IL10 Proteintech 60269-1-Ig WB,IHC,IF/ICC, ELISA Rabbit anti-ARG1 Proteintech 16001-1-AP WB, IHC, IF/ICC, IF-P, IP, ELISA Mouse anti-GAPDH Proteintech 60004-1-Ig WB,IF/ICC,FC (Intra), IP, ELISA Rabbit anti-β-ACTIN ABclonal AC026 WB,IHC-P,ELISA Hematoxylin and eosin (H&E) staining Hematoxylin and eosin was conducted following the procedure previously outlined[81]. .. Cell culture and transfection The AU565, UACC812, and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/high glucose (Meilune, Dalian, China) or RPMI-1640 (Meilune, Dalian, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA) and 1% penicillin–streptomycin (Meilune, Dalian, China).
Staining:Article Title: Mechanism of IL-4 mediated RPL19 promoting malignant progression in HER2 positive breast cancer.
Article Snippet: .. Antibody Source Catalog number Applications Rabbit anti-RPL19 Proteintech 14701-1-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Mouse anti-IL4 Proteintech 66142-1-Ig WB,IHC,IF-P,FC (Intra), ELISA Rabbit anti-JUN Proteintech 24909-1-AP WB, IHC, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT6 Proteintech 51073-1-AP WB, IHC, ELISA Rabbit anti-p-STAT6 Affiniyt AF3301 WB,IHC,IF/ICC Rabbit anti-AKT Proteintech 10176-2-AP WB, IHC, IF-P, IF-Fro, IP, ELISA Rabbit anti-p-AKT Proteintech 66444-1-Ig WB, IHC, IF/ICC, FC (Intra), ELISA AR TIC LE IN PR ES S Rabbit anti-MAPK Proteintech 14064-1-AP WB, IHC, ELISA Rabbit anti-p-MAPK Proteintech 80024-1-RR WB, IF/ICC, FC (Intra), ELISA Rabbit anti-STAT3 Proteintech 10253-2-AP WB, IHC, IF/ICC, FC (Intra), IP, ELISA Rabbit anti-p-STAT3 Proteintech 28945-1-AP WB, ELISA Rabbit anti-IL1-β Proteintech 26048-1-AP WB, ELISA Rabbit anti-TNF-α Proteintech 17590-1-AP WB, ELISA Mouse anti-IL10 Proteintech 60269-1-Ig WB,IHC,IF/ICC, ELISA Rabbit anti-ARG1 Proteintech 16001-1-AP WB, IHC, IF/ICC, IF-P, IP, ELISA Mouse anti-GAPDH Proteintech 60004-1-Ig WB,IF/ICC,FC (Intra), IP, ELISA Rabbit anti-β-ACTIN ABclonal AC026 WB,IHC-P,ELISA Hematoxylin and eosin (H&E) staining Hematoxylin and eosin was conducted following the procedure previously outlined[81]. .. Cell culture and transfection The AU565, UACC812, and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM)/high glucose (Meilune, Dalian, China) or RPMI-1640 (Meilune, Dalian, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA) and 1% penicillin–streptomycin (Meilune, Dalian, China).
Saline:Article Title: Translation factor eIF4G2 directs CD8 + T cell lineage commitment by selectively enabling the IL-7 receptor response
Article Snippet: Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% non-fat milk or bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 1 hour at room temperature and then incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: eIF4G2 (CST, 3468S), β-actin (CST, 3700S), eIF4G1 (Proteintech, 15704-1-AP), IL-7Rα (Proteintech, 17626-1-AP), γc (Proteintech, 11409-1-AP), STAT5 (Proteintech, 13179-1-AP), phospho STAT5 (CST, 4322T), STAT6 (Proteintech, 51073-1-AP), mouse phospho STAT6 (CST, 56554S). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Proteintech, SA00001-1; SA00001-9) for 1 hour at room temperature.
Incubation:Article Title: Translation factor eIF4G2 directs CD8 + T cell lineage commitment by selectively enabling the IL-7 receptor response
Article Snippet: Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% non-fat milk or bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 1 hour at room temperature and then incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: eIF4G2 (CST, 3468S), β-actin (CST, 3700S), eIF4G1 (Proteintech, 15704-1-AP), IL-7Rα (Proteintech, 17626-1-AP), γc (Proteintech, 11409-1-AP), STAT5 (Proteintech, 13179-1-AP), phospho STAT5 (CST, 4322T), STAT6 (Proteintech, 51073-1-AP), mouse phospho STAT6 (CST, 56554S). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Proteintech, SA00001-1; SA00001-9) for 1 hour at room temperature.
Article Title: Orcinol glucoside ameliorates pulmonary fibrosis by suppressing hyaluronic acid synthesis and macrophage M2 polarization via targeting hyaluronic acid synthase 2
Article Snippet: Antibodies for Collagen type I α 1 chain (COL1A1; cat. no. 72026; 1:1,000), Phospho-STAT6 (Tyr641; cat. no. 56554S; 1:1,000) and α-smooth muscle actin (α-SMA; cat. no. 19245; 1:1,000) were obtained from Cell Signaling Technology, Inc. .. The HAS2 (cat. no. bs-11290R; 1:1,000) antibody was purchased from BIOSS and HRP-conjugated β-tubulin (cat. no. HRP-66240: 1:20,000) and STAT6 (cat. no. 51073-1-AP; 1:5,000) antibodies were obtained from Proteintech Group, Inc. Membranes were incubated for 1 h at room temperature with an HRP-conjugated secondary antibody (Cell Signaling Technology, Inc.; 1:2,000) following TBS-T (containing 0.05% Tween-20) washing. .. Protein bands were detected using Immobilon Western Chemiluminescent substrate (MilliporeSigma) and imaged with a Tanon 5200 Multi automatic chemiluminescence system (Tanon Science and Technology Co., Ltd.).
Article Title: IL-37 Ameliorates Chronic Endometritis by Attenuating Epithelial-Mesenchymal Transition and Promoting M2 Macrophage Polarization.
Article Snippet: .. The sections were then incubated overnight at 4 ◦C with primary antibodies against the following proteins: IL-37 (Abcam, Cambridge, UK), E-cadherin (Cell Signaling Technology [CST], Danvers, MA, USA), vimentin (ABclonal, Woburn, MA, USA), CD86 (Proteintech, Rosemont, IL, USA), CD206 (Proteintech), Smad3 (CST), and STAT6 (Proteintech). ..
Blocking Assay:Article Title: Translation factor eIF4G2 directs CD8 + T cell lineage commitment by selectively enabling the IL-7 receptor response
Article Snippet: Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Membranes were blocked with 5% non-fat milk or bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 1 hour at room temperature and then incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: eIF4G2 (CST, 3468S), β-actin (CST, 3700S), eIF4G1 (Proteintech, 15704-1-AP), IL-7Rα (Proteintech, 17626-1-AP), γc (Proteintech, 11409-1-AP), STAT5 (Proteintech, 13179-1-AP), phospho STAT5 (CST, 4322T), STAT6 (Proteintech, 51073-1-AP), mouse phospho STAT6 (CST, 56554S). .. After washing with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibody (Proteintech, SA00001-1; SA00001-9) for 1 hour at room temperature.
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