Review



xbai site  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc xbai site
    Xbai Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/STARR-seq+luciferase+validation+vector_ORI_empty+(Plasmid+%2399297)/pmc12780183-479-8-11
    Average 93 stars, based on 8 article reviews
    xbai site - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Luciferase:

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model.
    Article Snippet: .. The STARR-seq luciferase reporter plasmid (vector_ORI_empty) was a gift from Alexander Stark (Addgene plasmid #99297; https://www.addgene.org/99297; RRID: Addgene_992927) 64. ..

    Article Title: The non-canonical poly(A) polymerase FAM46C promotes erythropoiesis.
    Article Snippet: The post-transcriptional regulation of mRNA is a crucial component of gene expression.. The disruption of this process has detrimental effects on the normal development and gives rise to various diseases.. Searching for novel post-transcriptional regulators and exploring their roles are essential for understanding development and disease.

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model
    Article Snippet: .. The STARR-seq luciferase reporter plasmid (vector_ORI_empty) was a gift from Alexander Stark (Addgene plasmid #99297; https://www.addgene.org/99297 ; RRID: Addgene_992927) . ..

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: .. Enhancers were PCR-cloned from genomic DNA into the STARR-seq firefly luciferase validation vector ORI_empty (RRID: Addgene_99297). .. Vectors ORI_SV40 (RRID: Addgene_99309) and ORI_neg.cont (RRID: Addgene_99315) were used as positive and negative controls.

    Article Title: Dynamic HSF2 regulation drives breast cancer progression by steering the balance between proliferation and invasion
    Article Snippet: Following two weeks of culturing, the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems). .. The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). ..

    Plasmid Preparation:

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model.
    Article Snippet: .. The STARR-seq luciferase reporter plasmid (vector_ORI_empty) was a gift from Alexander Stark (Addgene plasmid #99297; https://www.addgene.org/99297; RRID: Addgene_992927) 64. ..

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model
    Article Snippet: .. The STARR-seq luciferase reporter plasmid (vector_ORI_empty) was a gift from Alexander Stark (Addgene plasmid #99297; https://www.addgene.org/99297 ; RRID: Addgene_992927) . ..

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model
    Article Snippet: .. The 11X EBV TR was cloned into the XbaI site of Addgene #99297 plasmid using T4 DNA ligase (NEB, Cat. No. M0202). ..

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: .. Enhancers were PCR-cloned from genomic DNA into the STARR-seq firefly luciferase validation vector ORI_empty (RRID: Addgene_99297). .. Vectors ORI_SV40 (RRID: Addgene_99309) and ORI_neg.cont (RRID: Addgene_99315) were used as positive and negative controls.

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model.
    Article Snippet: .. The 11X EBV TR was cloned into the XbaI site of Addgene #99297 plasmid using T4 DNA ligase (NEB, Cat. No. M0202). ..

    Article Title: Dynamic HSF2 regulation drives breast cancer progression by steering the balance between proliferation and invasion
    Article Snippet: Following two weeks of culturing, the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems). .. The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). ..

    other:

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: Vectors ORI_SV40 (RRID: Addgene_99309) and ORI_neg.cont (RRID: Addgene_99315) were used as positive and negative controls.

    Polymerase Chain Reaction:

    Article Title: The non-canonical poly(A) polymerase FAM46C promotes erythropoiesis.
    Article Snippet: The post-transcriptional regulation of mRNA is a crucial component of gene expression.. The disruption of this process has detrimental effects on the normal development and gives rise to various diseases.. Searching for novel post-transcriptional regulators and exploring their roles are essential for understanding development and disease.

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: .. Enhancers were PCR-cloned from genomic DNA into the STARR-seq firefly luciferase validation vector ORI_empty (RRID: Addgene_99297). .. Vectors ORI_SV40 (RRID: Addgene_99309) and ORI_neg.cont (RRID: Addgene_99315) were used as positive and negative controls.

    Cloning:

    Article Title: The non-canonical poly(A) polymerase FAM46C promotes erythropoiesis.
    Article Snippet: The post-transcriptional regulation of mRNA is a crucial component of gene expression.. The disruption of this process has detrimental effects on the normal development and gives rise to various diseases.. Searching for novel post-transcriptional regulators and exploring their roles are essential for understanding development and disease.

    Article Title: Dynamic HSF2 regulation drives breast cancer progression by steering the balance between proliferation and invasion
    Article Snippet: Following two weeks of culturing, the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems). .. The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). ..

    Clone Assay:

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model
    Article Snippet: .. The 11X EBV TR was cloned into the XbaI site of Addgene #99297 plasmid using T4 DNA ligase (NEB, Cat. No. M0202). ..

    Article Title: Discovering the complete enhancer map of human herpesviruses using a natural language processing model.
    Article Snippet: .. The 11X EBV TR was cloned into the XbaI site of Addgene #99297 plasmid using T4 DNA ligase (NEB, Cat. No. M0202). ..

    Biomarker Discovery:

    Article Title: Selective Enhancer Dependencies in MYC-Intact and MYC-Rearranged Germinal Center B-cell Diffuse Large B-cell Lymphoma
    Article Snippet: .. Enhancers were PCR-cloned from genomic DNA into the STARR-seq firefly luciferase validation vector ORI_empty (RRID: Addgene_99297). .. Vectors ORI_SV40 (RRID: Addgene_99309) and ORI_neg.cont (RRID: Addgene_99315) were used as positive and negative controls.

    Article Title: Dynamic HSF2 regulation drives breast cancer progression by steering the balance between proliferation and invasion
    Article Snippet: Following two weeks of culturing, the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems). .. The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). ..

    Generated:

    Article Title: Dynamic HSF2 regulation drives breast cancer progression by steering the balance between proliferation and invasion
    Article Snippet: Following two weeks of culturing, the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems). .. The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). ..



    Similar Products

    starr seq vector 596  (Addgene inc)
    95
    Addgene inc starr seq vector 596
    Starr Seq Vector 596, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/hSTARR-seq_ORI+vector+(Plasmid+%2399296)/pm41514212-344-21-25
    Average 95 stars, based on 1 article reviews
    starr seq vector 596 - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    starr seq vector  (Addgene inc)
    95
    Addgene inc starr seq vector
    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of <t>the</t> <t>STARR-seq</t> oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis
    Starr Seq Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/hSTARR-seq_ORI+vector+(Plasmid+%2399296)/pmc12879379-317-20-23
    Average 95 stars, based on 1 article reviews
    starr seq vector - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    alexander stark  (Addgene inc)
    93
    Addgene inc alexander stark
    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of <t>the</t> <t>STARR-seq</t> oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis
    Alexander Stark, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/STARR-seq+luciferase+validation+vector_ORI_empty+(Plasmid+%2399297)/pmc12780183-476-10-12
    Average 93 stars, based on 1 article reviews
    alexander stark - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    xbai site  (Addgene inc)
    93
    Addgene inc xbai site
    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of <t>the</t> <t>STARR-seq</t> oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis
    Xbai Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/STARR-seq+luciferase+validation+vector_ORI_empty+(Plasmid+%2399297)/pmc12780183-479-8-11
    Average 93 stars, based on 1 article reviews
    xbai site - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    starr seq ori vector  (Addgene inc)
    95
    Addgene inc starr seq ori vector
    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of <t>the</t> <t>STARR-seq</t> oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis
    Starr Seq Ori Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/hSTARR-seq_ORI+vector+(Plasmid+%2399296)/pmc12652976-122-1-4
    Average 95 stars, based on 1 article reviews
    starr seq ori vector - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    starr seq backbone  (Addgene inc)
    95
    Addgene inc starr seq backbone
    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of <t>the</t> <t>STARR-seq</t> oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis
    Starr Seq Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+vector/hSTARR-seq_ORI+vector+(Plasmid+%2399296)/pm40825976-97-8-10
    Average 95 stars, based on 1 article reviews
    starr seq backbone - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of the STARR-seq oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis

    Journal: BMC Genomics

    Article Title: Identification of novel DNA sequence motifs that modulate transcription in T cells

    doi: 10.1186/s12864-025-12425-9

    Figure Lengend Snippet: Novel motifs affect gene transcription in Jurkats. A Shown, is a diagram of the STARR-seq oligo library design. The figure was adapted from Georgakopoulos-Soares et al under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ . Changes were made to the figure to account for the differences in our overall library design. Eighteen candidate motifs (nine novel and nine TFBS motifs) were embedded in a synthetic background DNA sequence. Each sequence contained one to three instances of a motif, separated by 10bp. The oligonucleotide library contains every possible combination, position, and orientation of the 18 candidate motifs. B The mean activity score for the 18 motifs in their respective orientations for Jurkat and ( C ) K562 cells are shown above. Mean activity scores were obtained from oligos containing the motif of interest only in its indicated orientation. Motifs with a significant effect on gene transcription were identified using a two-sided t-test that compared oligos containing the motif of interest to those that do not contain the motif. Only oligos containing 1-3 instances of the given motif in its respective orientation were used. Motifs with a significant effect of gene transcription are indicated as solid bars (Bonferroni corrected, p -value < 0.001, |Cohen's D| > 0.5). Error bars represent the standard error of activity scores. D Linear modelling was used to determine if there was an association between the number of copies of a motif and the mean activity score for the motifs with a significant effect on gene transcription in Jurkat cells and ( E ) K562 cells (Bonferroni corrected, p -value < 0.007). Only oligos containing one to three copies of the motif of interest were included in the analysis

    Article Snippet: After designing the library, the oligonucleotide sequences were flanked by AgeI and SalI restriction sites to facilitate cloning into the STARR-seq vector (hSTARR-seq_ORI, Addgene #99296) and the necessary priming and Illumina adapter sequences were added to enable sequencing (Supplemental Table S3).

    Techniques: Sequencing, Activity Assay