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scramble nonsense control shrna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology scramble nonsense control shrna
    <t>Nrf2</t> activation mediates 4-octyl itaconate-induced neuronal cell protection against H 2 O 2 . SH-SY5Y cells ( a - e ) or the primary murine neurons ( i - k ), with the applied Nrf2 <t>shRNA</t> or the scramble control shRNA (“shC”), were either untreated or treated with 4-octyl itaconate (OI), mRNA and protein expression of listed genes were shown ( a - c , and i ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability (CCK-8 OD, d ), cell death (LDH release, j ) and apoptosis (TUNEL ratio increase, e , and k ) were tested. Stable SH-SY5Y cells, with the CRISPR/Cas9-Nrf2 KO construct (“Nrf2-KO”) or the CRISPR/Cas9 control construct (“Cas9-c”), were treated with 4-octyl itaconate (OI), listed proteins were shown ( f ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability ( g ) and apoptosis ( h ) were tested. Expression of listed proteins were quantified and normalized to the loading control ( c , f and i ). “shNrf2 (m)” stands for murine Nrf2 shRNA ( I - K ). Bars stand for mean ± standard deviation (S.D., n = 5). # P < 0.05 vs. “shC” cells ( a , b , d and e ). # P < 0.05 ( g , h , j and k )
    Scramble Nonsense Control Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/standard+scrambled+sequence/Control+shRNA+Lentiviral+Particles-A/pmc06238317-86-28-37
    Average 96 stars, based on 1301 article reviews
    scramble nonsense control shrna - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide"

    Article Title: Four-octyl itaconate activates Keap1-Nrf2 signaling to protect neuronal cells from hydrogen peroxide

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-018-0294-2

    Nrf2 activation mediates 4-octyl itaconate-induced neuronal cell protection against H 2 O 2 . SH-SY5Y cells ( a - e ) or the primary murine neurons ( i - k ), with the applied Nrf2 shRNA or the scramble control shRNA (“shC”), were either untreated or treated with 4-octyl itaconate (OI), mRNA and protein expression of listed genes were shown ( a - c , and i ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability (CCK-8 OD, d ), cell death (LDH release, j ) and apoptosis (TUNEL ratio increase, e , and k ) were tested. Stable SH-SY5Y cells, with the CRISPR/Cas9-Nrf2 KO construct (“Nrf2-KO”) or the CRISPR/Cas9 control construct (“Cas9-c”), were treated with 4-octyl itaconate (OI), listed proteins were shown ( f ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability ( g ) and apoptosis ( h ) were tested. Expression of listed proteins were quantified and normalized to the loading control ( c , f and i ). “shNrf2 (m)” stands for murine Nrf2 shRNA ( I - K ). Bars stand for mean ± standard deviation (S.D., n = 5). # P < 0.05 vs. “shC” cells ( a , b , d and e ). # P < 0.05 ( g , h , j and k )
    Figure Legend Snippet: Nrf2 activation mediates 4-octyl itaconate-induced neuronal cell protection against H 2 O 2 . SH-SY5Y cells ( a - e ) or the primary murine neurons ( i - k ), with the applied Nrf2 shRNA or the scramble control shRNA (“shC”), were either untreated or treated with 4-octyl itaconate (OI), mRNA and protein expression of listed genes were shown ( a - c , and i ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability (CCK-8 OD, d ), cell death (LDH release, j ) and apoptosis (TUNEL ratio increase, e , and k ) were tested. Stable SH-SY5Y cells, with the CRISPR/Cas9-Nrf2 KO construct (“Nrf2-KO”) or the CRISPR/Cas9 control construct (“Cas9-c”), were treated with 4-octyl itaconate (OI), listed proteins were shown ( f ); Cells were pretreated for 30 min with OI (25 μM), followed by stimulation of H 2 O 2 (300 μM) for indicated time, cell viability ( g ) and apoptosis ( h ) were tested. Expression of listed proteins were quantified and normalized to the loading control ( c , f and i ). “shNrf2 (m)” stands for murine Nrf2 shRNA ( I - K ). Bars stand for mean ± standard deviation (S.D., n = 5). # P < 0.05 vs. “shC” cells ( a , b , d and e ). # P < 0.05 ( g , h , j and k )

    Techniques Used: Activation Assay, shRNA, Control, Expressing, CCK-8 Assay, TUNEL Assay, CRISPR, Construct, Standard Deviation

    Related Articles

    shRNA:

    Article Title: METTL16 supports arginine biosynthesis and suppresses IL-1β production to attenuate allergic asthma
    Article Snippet: Upon reaching 50% confluency, the cells were incubated with complete growth medium containing 5 μg/mL polybrene (Yeasen, 40804ES76, Shanghai, China). .. The cells were then transducedwith either METTL16-targeting shRNA lentiviruses (Santa Cruz Biotechnology, sc-149379-V, CA, USA) or nontargeting control shRNA lentiviruses (Santa Cruz Biotechnology, sc-108080, CA, USA). ..

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: CXCR4, CXCR7 and PBRM1 are responsible for everolimus and cabozantinib resistance in human renal cancer cells.
    Article Snippet: .. Control shRNA Lentiviral Particles-A (2.5x104 IFU; shControl, sc-108080, Santa Cruz Biotechnology, Dallas, TX, USA) were used. ..

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle
    Article Snippet: GsMTx4 (Pz inhibitor; HY-P1410) was purchased from MedChemExpress (NJ, USA). .. Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA). ..

    Article Title: Adrenomedullin 2 drives intratumoral cancer cell invasion, intravasation and metastasis.
    Article Snippet: .. Scramble non targeting shRNA (sc-108080), ADM2 (sc-72452-V) and ATF2 (sc-29205-V) were purchased from Santa Cruz Biotechnologies. .. Lectin-649 (VECTDL1048) was from Vector Laboratories.

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Control:

    Article Title: METTL16 supports arginine biosynthesis and suppresses IL-1β production to attenuate allergic asthma
    Article Snippet: Upon reaching 50% confluency, the cells were incubated with complete growth medium containing 5 μg/mL polybrene (Yeasen, 40804ES76, Shanghai, China). .. The cells were then transducedwith either METTL16-targeting shRNA lentiviruses (Santa Cruz Biotechnology, sc-149379-V, CA, USA) or nontargeting control shRNA lentiviruses (Santa Cruz Biotechnology, sc-108080, CA, USA). ..

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: CXCR4, CXCR7 and PBRM1 are responsible for everolimus and cabozantinib resistance in human renal cancer cells.
    Article Snippet: .. Control shRNA Lentiviral Particles-A (2.5x104 IFU; shControl, sc-108080, Santa Cruz Biotechnology, Dallas, TX, USA) were used. ..

    Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle
    Article Snippet: GsMTx4 (Pz inhibitor; HY-P1410) was purchased from MedChemExpress (NJ, USA). .. Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA). ..

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Transduction:

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).

    Article Title: A natural product-derived degrader (COT-01): cortisone-based CRBN recruitment for cyclin D2 degradation in multiple myeloma.
    Article Snippet: .. MM.1S cells were transduced with lentiviral vectors encoding either a non-targeting control shRNA or an shRNA targeting human CRBN (sc108080, sc-78528-V, Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Sequencing:

    Article Title: Context-Dependent Associations of HO-1 Modulation with Ferroptosis-Associated Injury and Vascular Cell Functions In Vitro.
    Article Snippet: HUVEC cells were seeded one day before transduction, after which 5 μg/mL polybrene (Sigma) and the lentiviral transduction particles were added. .. Control cells were transduced with control shRNA encoding a scrambled sequence using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Article Title: Metformin and caffeic acid phenethyl ester counteract hyperglycemia-induced endothelial dysfunction through growth differentiation factor 15 and heme oxygenase-1-associated signaling.
    Article Snippet: Following this, 5 μg/mL of polybrene (Sigma-Aldrich Co.) and the lentiviral particles were introduced. .. Control cells were transduced with a control shRNA encoding a scrambled sequence, using transduction particles (sc-108080, Santa Cruz Biotechnology). ..

    Knockdown:

    Article Title: CLIC3 is upregulated across all subtypes of breast cancer and plays a key role in cell migration, invasion and growth in soft agar.
    Article Snippet: .. To produce knockdown cell lines, cells were transduced with a lentivirus containing shRNAs targeting LCN2 (shLCN2; also known as NGAL, sc-43969-V, Santa Cruz, Dallas, TX, USA) or CLIC3 (shCLIC3; sc-62126-V; Santa Cruz), as well as a scrambled shRNA control (shControl; sc-108080; Santa Cruz). .. Twenty-four hours after transduction, culture media was replaced with fresh media containing puromycin (Sigma-Aldrich, P8833, 5 g/mL).



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