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iq5 software  (Bio-Rad)


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    Bio-Rad iq5 software
    Iq5 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/standard+c+++program/ChromLab+Software/pmc03044099-81-10-11
    Average 96 stars, based on 3491 article reviews
    iq5 software - by Bioz Stars, 2026-09
    96/100 stars

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    Software:

    Article Title: Quantification of major royal jelly protein variants and concurrent fraud identification to confirm royal jelly quality employing the native LC-SEC-UV methodology
    Article Snippet: Traditional protein assays and 10-Hydroxy-2-decenoic acid (10-HDA) analysis are inefficient for assessing royal jelly (RJ) freshness and authenticity.. Structural integrities, thus efficacies of the Major Royal Jelly Proteins (MRJPs) might be modified based on storage conditions.. Total protein assays are incapable of identifying MRJP aggregation or degradation.

    Article Title: CD46 is a cellular receptor for species D human adenovirus.
    Article Snippet: .. Using an NGC chromatography system (BioRad) with the ChromLab software, the sample was bound to an anion exchange column (HiTrap Q FF, Cytiva). ..

    Article Title: Surface functionalization with short PAS-sequence affects H-ferritin nanocage stability.
    Article Snippet: .. Fractions of 0.5 mL were collected by monitoring the chromatogram from the integrated ChromLab Software (Bio-Rad). .. PAS20-HFn and PAS40-HFn collected fractions were quantified employing NanoDrop 2000c (Thermo Fisher Scientific) by 280 nm absorbance (A280) and PierceTM Bradford Plus Protein Assay Reagent (Thermo Fisher), according to manufacturing specifications.

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes.
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl2, 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl 2 , 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: CD46 is a cellular receptor for species D human adenovirus
    Article Snippet: .. Using an NGC chromatography system (BioRad) with the ChromLab software, the sample was bound to an anion exchange column (HiTrap Q FF, Cytiva). ..

    Article Title: Engineered antibodies that stabilize drug-modified KRAS G12C neoantigens enable selective and potent cross-HLA immunotherapy.
    Article Snippet: .. Solubilized β2m was loaded onto a 5-mL HisTrap HP immobilizedmetal affinity chromatography (IMAC) column (Cytiva, 17-5248- 02) installed on a NGC Discover (Bio-Rad, 7880009) operated using ChromLab software (Bio-Rad, v6.1.29.0). ..

    Article Title: Engineered antibodies that stabilize drug-modified KRAS G12C neoantigens enable selective and potent cross-HLA immunotherapy
    Article Snippet: .. Solubilized β2m was loaded onto a 5-mL HisTrap HP immobilized metal affinity chromatography (IMAC) column (Cytiva, 17-5248-02) installed on a NGC Discover (Bio-Rad, 7880009) operated using ChromLab software (Bio-Rad, v6.1.29.0). ..

    Chromatography:

    Article Title: CD46 is a cellular receptor for species D human adenovirus.
    Article Snippet: .. Using an NGC chromatography system (BioRad) with the ChromLab software, the sample was bound to an anion exchange column (HiTrap Q FF, Cytiva). ..

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes.
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl2, 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl 2 , 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: CD46 is a cellular receptor for species D human adenovirus
    Article Snippet: .. Using an NGC chromatography system (BioRad) with the ChromLab software, the sample was bound to an anion exchange column (HiTrap Q FF, Cytiva). ..

    Purification:

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes.
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl2, 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl 2 , 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Size-exclusion Chromatography:

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes.
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl2, 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Article Title: Tau phosphorylation impedes functionality of protective tau envelopes
    Article Snippet: The next day, the cleaved protein was collected and concentrated by spinning the sample at 3,500 rpm at 4 °C using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck). .. The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC chromatography system (Bio-Rad), equipped with ChromLab software (Bio-Rad), in 50 mM HEPES pH 7.4, 2 mM MgCl 2 , 1 mM EGTA, 150 mM KCl, 1 mM DTT, 0.1 mM ATP and 1 mM EDTA. .. Collected peak fractions were concentrated using a 50-kDa (for full-length tau) or 10-kDa (for tau-∆N) centrifugal filter tube (Amicon Ultra-15, Merck).

    Affinity Chromatography:

    Article Title: Engineered antibodies that stabilize drug-modified KRAS G12C neoantigens enable selective and potent cross-HLA immunotherapy.
    Article Snippet: .. Solubilized β2m was loaded onto a 5-mL HisTrap HP immobilizedmetal affinity chromatography (IMAC) column (Cytiva, 17-5248- 02) installed on a NGC Discover (Bio-Rad, 7880009) operated using ChromLab software (Bio-Rad, v6.1.29.0). ..

    Article Title: Engineered antibodies that stabilize drug-modified KRAS G12C neoantigens enable selective and potent cross-HLA immunotherapy
    Article Snippet: .. Solubilized β2m was loaded onto a 5-mL HisTrap HP immobilized metal affinity chromatography (IMAC) column (Cytiva, 17-5248-02) installed on a NGC Discover (Bio-Rad, 7880009) operated using ChromLab software (Bio-Rad, v6.1.29.0). ..



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    Pulse sequence of the carbon direct detected 3D-(H)CC-TOCSY-H1′C1′ experiment with a virtual decoupling scheme in t 3 . Narrow and wide filled bars correspond to rectangular 90° and 180° pulses, respectively. Selective pulses and gradients are indicated as semi - ellipses . The default pulse phase is x. The proton carrier frequency is centred at the water frequency (5.7 ppm). The values for the 13 C and 15 N offsets are set to 77 ppm ( 13 C Ribose ), 90 ppm ( 13 C C1′ ), 70 ppm ( 13 C C2′ ) and 160 ppm ( 15 N), respectively. Asynchronous GARP decoupling (Shaka et al. ) is used to suppress heteronuclear scalar coupling during acquisition. The pulse field gradients with a length of 1 ms have a smoothed square amplitude (Bruker Topspin 2.0, 2006). They are applied along the z -axis and have the following strengths: G 1 :16%, G 2 :16% (pulse length 300 μs), G 3 :80%, G 4 :70%, G 5 :10%, G 6 :50%, G 7 :60%. 100% of gradient strength corresponds to 53.5 Gauss/cm. Fixed delays are adjusted as follows: Δ = 3 ms (1/(2* 1 J HC )), T = 6.26 ms (1/(4* 1 J CC ). For the CC-TOCSY transfer the FLOPSY-16 mixing sequence (Kadkhodaie et al. ) was applied, optimized for a single transfer (τ M = 3 ms) or multiple transfers (τ M = 15 ms). For the virtual decoupling scheme, a band selective pulse 180° Q3 Gaussian cascade (Emsley and Bodenhausen ) of 2 ms (semi-ellipse) is applied either on C1′ or C2′. Phase cycling: φ 1 = x, − x, φ 2 = 2(x), 2(−x), φ 3 IP = 4(y), 4(−y), φ 3 AP = 4(x), 4(−x), φ rec = x, 2(−x), x, −x, 2(x), −x. Quadrature detection in the ω 1 and ω 2 dimensions is obtained by incrementing φ 1 and φ 2 in a States-TPPI manner (Marion et al. ). The in-phase and anti-phase components of the C1′C2′ coherences were recorded in an interleaved manner and afterwards combined with a standard Bruker c-program (au-prog: splitcomb) using the parameter: ipap, inphase/antiphase correction term of 1.09 and an average coupling constant 1 J(C1′C2′) = 40 Hz

    Journal: Journal of Biomolecular Nmr

    Article Title: 13 C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides

    doi: 10.1007/s10858-010-9429-5

    Figure Lengend Snippet: Pulse sequence of the carbon direct detected 3D-(H)CC-TOCSY-H1′C1′ experiment with a virtual decoupling scheme in t 3 . Narrow and wide filled bars correspond to rectangular 90° and 180° pulses, respectively. Selective pulses and gradients are indicated as semi - ellipses . The default pulse phase is x. The proton carrier frequency is centred at the water frequency (5.7 ppm). The values for the 13 C and 15 N offsets are set to 77 ppm ( 13 C Ribose ), 90 ppm ( 13 C C1′ ), 70 ppm ( 13 C C2′ ) and 160 ppm ( 15 N), respectively. Asynchronous GARP decoupling (Shaka et al. ) is used to suppress heteronuclear scalar coupling during acquisition. The pulse field gradients with a length of 1 ms have a smoothed square amplitude (Bruker Topspin 2.0, 2006). They are applied along the z -axis and have the following strengths: G 1 :16%, G 2 :16% (pulse length 300 μs), G 3 :80%, G 4 :70%, G 5 :10%, G 6 :50%, G 7 :60%. 100% of gradient strength corresponds to 53.5 Gauss/cm. Fixed delays are adjusted as follows: Δ = 3 ms (1/(2* 1 J HC )), T = 6.26 ms (1/(4* 1 J CC ). For the CC-TOCSY transfer the FLOPSY-16 mixing sequence (Kadkhodaie et al. ) was applied, optimized for a single transfer (τ M = 3 ms) or multiple transfers (τ M = 15 ms). For the virtual decoupling scheme, a band selective pulse 180° Q3 Gaussian cascade (Emsley and Bodenhausen ) of 2 ms (semi-ellipse) is applied either on C1′ or C2′. Phase cycling: φ 1 = x, − x, φ 2 = 2(x), 2(−x), φ 3 IP = 4(y), 4(−y), φ 3 AP = 4(x), 4(−x), φ rec = x, 2(−x), x, −x, 2(x), −x. Quadrature detection in the ω 1 and ω 2 dimensions is obtained by incrementing φ 1 and φ 2 in a States-TPPI manner (Marion et al. ). The in-phase and anti-phase components of the C1′C2′ coherences were recorded in an interleaved manner and afterwards combined with a standard Bruker c-program (au-prog: splitcomb) using the parameter: ipap, inphase/antiphase correction term of 1.09 and an average coupling constant 1 J(C1′C2′) = 40 Hz

    Article Snippet: The double IPAP is applied in an interleaved manner and the linear combination of all four experiments (Fig. a) is performed after data acquisition by using the standard Bruker c-program splitcomb (included in Topspin 2.1).

    Techniques: Sequencing

    Double IPAP pulse scheme for RNA virtual decoupling of 1 J(C3′,C4′) and 1 J(C4′,C5′) illustrated on a 13 C, 15 N-labelled UTP sample. The offset values for 13 C are set to 77 ppm ( 13 C Ribose ), 83.3 ppm ( 13 C C4′ ), 69.4 ppm ( 13 C C3′ ), and 64.7 ppm ( 13 C C5′ ), respectively. The pulse field gradient of 1 ms length has a smoothed square amplitude (Bruker Topspin 2.0, 2006). It is applied along the z -axis and has the following strength: G 1 : 31%. 100% of gradient strength corresponds to 53.5 Gauss/cm. Fixed delays are adjusted as follows: T = 6.26 ms (1/(4* 1 J CC ). φ IPIP = x, −x, φ IPAP = −y, y, φ APIP = −y, y, φ APAP = −x, x, φ rec = −x, x. For the virtual decoupling, the following band selective pulses are applied: for C4′ (83 ppm ± 3 ppm) a 6.5 ms RE-BURP (Geen and Freeman ), for C3′ & C4′ (76 ppm ± 9.5 ppm) a 2 ms RE-BURP, for C4′ & C5′ (83 ppm ± 3 ppm & 64 ppm ± 3 ppm) a band-selective-Bloch-Siegert-compensated RE-BURP pulse with a length of 6.5 ms(RE-BURP_C4C5bs). The 1D spectrum a shows the linear combination of all four spectra using the c-prog splitcomb (included in Bruker Topspin 2.1). The doted lines indicate the 1D spectrum b , resulting from the linear combination of only the two spectra (IPIP and APAP)

    Journal: Journal of Biomolecular Nmr

    Article Title: 13 C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides

    doi: 10.1007/s10858-010-9429-5

    Figure Lengend Snippet: Double IPAP pulse scheme for RNA virtual decoupling of 1 J(C3′,C4′) and 1 J(C4′,C5′) illustrated on a 13 C, 15 N-labelled UTP sample. The offset values for 13 C are set to 77 ppm ( 13 C Ribose ), 83.3 ppm ( 13 C C4′ ), 69.4 ppm ( 13 C C3′ ), and 64.7 ppm ( 13 C C5′ ), respectively. The pulse field gradient of 1 ms length has a smoothed square amplitude (Bruker Topspin 2.0, 2006). It is applied along the z -axis and has the following strength: G 1 : 31%. 100% of gradient strength corresponds to 53.5 Gauss/cm. Fixed delays are adjusted as follows: T = 6.26 ms (1/(4* 1 J CC ). φ IPIP = x, −x, φ IPAP = −y, y, φ APIP = −y, y, φ APAP = −x, x, φ rec = −x, x. For the virtual decoupling, the following band selective pulses are applied: for C4′ (83 ppm ± 3 ppm) a 6.5 ms RE-BURP (Geen and Freeman ), for C3′ & C4′ (76 ppm ± 9.5 ppm) a 2 ms RE-BURP, for C4′ & C5′ (83 ppm ± 3 ppm & 64 ppm ± 3 ppm) a band-selective-Bloch-Siegert-compensated RE-BURP pulse with a length of 6.5 ms(RE-BURP_C4C5bs). The 1D spectrum a shows the linear combination of all four spectra using the c-prog splitcomb (included in Bruker Topspin 2.1). The doted lines indicate the 1D spectrum b , resulting from the linear combination of only the two spectra (IPIP and APAP)

    Article Snippet: The double IPAP is applied in an interleaved manner and the linear combination of all four experiments (Fig. a) is performed after data acquisition by using the standard Bruker c-program splitcomb (included in Topspin 2.1).

    Techniques: