7 aad staining solution  (Miltenyi Biotec)

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Staining, Expressing, Transmission Assay, Electron Microscopy, Microscopy, Control

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Expressing, Staining, Transmission Assay, Electron Microscopy, Microscopy, Control