Review




Structured Review

Servicebio Inc propidium iodide pi staining solution
Propidium Iodide Pi Staining Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/iodide+propidium/pmc13263685-246-7-12
Average 86 stars, based on 1 article reviews
propidium iodide pi staining solution - by Bioz Stars, 2026-11
86/100 stars

Images

Related Articles

Incubation:

Article Title: Targeting NADPH oxidase 2 suppresses the growth of esophageal squamous cell carcinoma by regulating BTG2 pathway.
Article Snippet: .. After incubation with propidium iodide (PI) solution (Cat. #1700, Servicebio, Wuhan, China) for 30 min at 37°C in the dark, the cells were measured using an EXFOLW-206 Flow Cytometry (DAKEWE, Shenzhen, China). ..

Article Title: Recombinant RGD-Apoptins Decrease Human Melanoma Cell Viability
Article Snippet: .. The medium was then replaced with OptiMEM containing recombinant apoptins at a concentration of 10 μM, and cells were incubated for 24 h. Cells were harvested by trypsinization, rinsed twice with HBSS (Capricorn Scientific, Ebsdorfergrund, Germany) and incubated with a mixture of propidium iodide (Servicebio, Wuhan, China) and APC Annexin V (Thermo Fisher Scientific, Waltham, MA, USA) for 15 min in dark. .. The cells were analyzed immediately after incubation via a NovoCyte Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Targeting NADPH oxidase 2 suppresses the growth of esophageal squamous cell carcinoma by regulating BTG2 pathway
Article Snippet: .. After incubation with propidium iodide (PI) solution (Cat. #1700, Servicebio, Wuhan, China) for 30 min at 37°C in the dark, the cells were measured using an EXFOLW-206 Flow Cytometry (DAKEWE, Shenzhen, China). ..

Flow Cytometry:

Article Title: Targeting NADPH oxidase 2 suppresses the growth of esophageal squamous cell carcinoma by regulating BTG2 pathway.
Article Snippet: .. After incubation with propidium iodide (PI) solution (Cat. #1700, Servicebio, Wuhan, China) for 30 min at 37°C in the dark, the cells were measured using an EXFOLW-206 Flow Cytometry (DAKEWE, Shenzhen, China). ..

Article Title: Targeting NADPH oxidase 2 suppresses the growth of esophageal squamous cell carcinoma by regulating BTG2 pathway
Article Snippet: .. After incubation with propidium iodide (PI) solution (Cat. #1700, Servicebio, Wuhan, China) for 30 min at 37°C in the dark, the cells were measured using an EXFOLW-206 Flow Cytometry (DAKEWE, Shenzhen, China). ..

Recombinant:

Article Title: Recombinant RGD-Apoptins Decrease Human Melanoma Cell Viability
Article Snippet: .. The medium was then replaced with OptiMEM containing recombinant apoptins at a concentration of 10 μM, and cells were incubated for 24 h. Cells were harvested by trypsinization, rinsed twice with HBSS (Capricorn Scientific, Ebsdorfergrund, Germany) and incubated with a mixture of propidium iodide (Servicebio, Wuhan, China) and APC Annexin V (Thermo Fisher Scientific, Waltham, MA, USA) for 15 min in dark. .. The cells were analyzed immediately after incubation via a NovoCyte Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA).

Concentration Assay:

Article Title: Recombinant RGD-Apoptins Decrease Human Melanoma Cell Viability
Article Snippet: .. The medium was then replaced with OptiMEM containing recombinant apoptins at a concentration of 10 μM, and cells were incubated for 24 h. Cells were harvested by trypsinization, rinsed twice with HBSS (Capricorn Scientific, Ebsdorfergrund, Germany) and incubated with a mixture of propidium iodide (Servicebio, Wuhan, China) and APC Annexin V (Thermo Fisher Scientific, Waltham, MA, USA) for 15 min in dark. .. The cells were analyzed immediately after incubation via a NovoCyte Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA).

Staining:

Article Title: Interleukin-6-Associated Pyroptosis, Apoptosis, and Necroptosis via JAKs/STAT3-RIPK1 Axis: A Potential Mechanism for CD4+ T-Cell Depletion in Bacterial Sepsis
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were cultured in Roswell Park Memorial Institute medium (RPMI)-1640 with 10% fetal bovine serum and treated with disulfiram ( DSF, 0.3 μM), Z-Val-Ala-Asp-fluoromethylketone (Z-VAD, 50 μM), or GSK’872 (10 μM) from Selleck Chemicals (Houston, TX) or MedChemExpress (MCE, Monmouth Junction, NJ). .. For cell death assessment, PBMCs were stained with ethidium homodimer III (EthD-III, PE, 40274ES60, Yeasen), propiddium iodide (PI, PE, G1021, Servicebio) and Hoechst 33342 (BV421, G1011, Servicebio, Wuhan, China), or processed with a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (PE-CF594, E-CK-A322, Elabscience, Wuhan, China) according to the manufacturers’ instructions. .. Fluorescence signals were acquired via flow cytometry and analyzed using FlowJo v10 (FlowJo, LLC, Ashland, OR), with gating strategies based on unstained controls (Figs. S1 and S2, https://links.lww.com/CCM/H912).

Article Title: Maternal methionine supplementation regulates skeletal muscle development through N 6 -methyladenosine mRNA methylation in broiler offspring
Article Snippet: .. After washing, the cells were stained with propidium iodide (PI) staining solution (Servicebio Technology Co., Ltd., Wuhan, Hubei, China) in the dark for 30 min at room temperature. .. Cell cycle distribution was then analyzed by flow cytometry, and the data were processed using ModFit LT software (Verity Software House, Topsham, ME, USA).

Article Title: TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.
Article Snippet: .. Propidium Iodide (PI) staining Cells cultured in 24-well plates were stained with PI (#G1021, Servicebio, Wuhan, China) in the dark for 10 min. Stained cells were observed and imaged using a Leica fluorescence microscope. .. The levels of MDA, GSH, and the LIP were measured using commercial assay kits: MDA with the Lipid Peroxidation MDA Assay Kit (#S0131S, Beyotime), GSH with the GSH and GSSG Assay Kit (#S0053, Beyotime), and LIP with the Cellular Red Fluorescent Ferrous Ion Assay Kit with RhoNox-6 (#S1070S, Beyotime).

Article Title: TGIF2-mediated HMGB3 overexpression promotes esophageal squamous cell carcinoma proliferation and metastasis through TLR3/TGF-β signaling
Article Snippet: .. After fixation in 75% ethanol for 1 h at 4 °C, the cells were washed with PBS and subsequently stained with 400 μL of propidium iodide (Servicebio; Wuhan, China) and 100 μL of RNase (BD Pharmingen; Guangzhou, China) in the dark at 37 °C for 30 min. .. After fixation in 75% ethanol for 1 h at 4 °C, the cells were washed with PBS and subsequently stained with 400 μL of propidium iodide (Servicebio; Wuhan, China) and 100 μL of RNase (BD Pharmingen; Guangzhou, China) in the dark at 37 °C for 30 min.

Ethidium Homodimer Assay:

Article Title: Interleukin-6-Associated Pyroptosis, Apoptosis, and Necroptosis via JAKs/STAT3-RIPK1 Axis: A Potential Mechanism for CD4+ T-Cell Depletion in Bacterial Sepsis
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were cultured in Roswell Park Memorial Institute medium (RPMI)-1640 with 10% fetal bovine serum and treated with disulfiram ( DSF, 0.3 μM), Z-Val-Ala-Asp-fluoromethylketone (Z-VAD, 50 μM), or GSK’872 (10 μM) from Selleck Chemicals (Houston, TX) or MedChemExpress (MCE, Monmouth Junction, NJ). .. For cell death assessment, PBMCs were stained with ethidium homodimer III (EthD-III, PE, 40274ES60, Yeasen), propiddium iodide (PI, PE, G1021, Servicebio) and Hoechst 33342 (BV421, G1011, Servicebio, Wuhan, China), or processed with a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (PE-CF594, E-CK-A322, Elabscience, Wuhan, China) according to the manufacturers’ instructions. .. Fluorescence signals were acquired via flow cytometry and analyzed using FlowJo v10 (FlowJo, LLC, Ashland, OR), with gating strategies based on unstained controls (Figs. S1 and S2, https://links.lww.com/CCM/H912).

TUNEL Assay:

Article Title: Interleukin-6-Associated Pyroptosis, Apoptosis, and Necroptosis via JAKs/STAT3-RIPK1 Axis: A Potential Mechanism for CD4+ T-Cell Depletion in Bacterial Sepsis
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were cultured in Roswell Park Memorial Institute medium (RPMI)-1640 with 10% fetal bovine serum and treated with disulfiram ( DSF, 0.3 μM), Z-Val-Ala-Asp-fluoromethylketone (Z-VAD, 50 μM), or GSK’872 (10 μM) from Selleck Chemicals (Houston, TX) or MedChemExpress (MCE, Monmouth Junction, NJ). .. For cell death assessment, PBMCs were stained with ethidium homodimer III (EthD-III, PE, 40274ES60, Yeasen), propiddium iodide (PI, PE, G1021, Servicebio) and Hoechst 33342 (BV421, G1011, Servicebio, Wuhan, China), or processed with a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (PE-CF594, E-CK-A322, Elabscience, Wuhan, China) according to the manufacturers’ instructions. .. Fluorescence signals were acquired via flow cytometry and analyzed using FlowJo v10 (FlowJo, LLC, Ashland, OR), with gating strategies based on unstained controls (Figs. S1 and S2, https://links.lww.com/CCM/H912).

Cell Culture:

Article Title: TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.
Article Snippet: .. Propidium Iodide (PI) staining Cells cultured in 24-well plates were stained with PI (#G1021, Servicebio, Wuhan, China) in the dark for 10 min. Stained cells were observed and imaged using a Leica fluorescence microscope. .. The levels of MDA, GSH, and the LIP were measured using commercial assay kits: MDA with the Lipid Peroxidation MDA Assay Kit (#S0131S, Beyotime), GSH with the GSH and GSSG Assay Kit (#S0053, Beyotime), and LIP with the Cellular Red Fluorescent Ferrous Ion Assay Kit with RhoNox-6 (#S1070S, Beyotime).

Fluorescence:

Article Title: TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.
Article Snippet: .. Propidium Iodide (PI) staining Cells cultured in 24-well plates were stained with PI (#G1021, Servicebio, Wuhan, China) in the dark for 10 min. Stained cells were observed and imaged using a Leica fluorescence microscope. .. The levels of MDA, GSH, and the LIP were measured using commercial assay kits: MDA with the Lipid Peroxidation MDA Assay Kit (#S0131S, Beyotime), GSH with the GSH and GSSG Assay Kit (#S0053, Beyotime), and LIP with the Cellular Red Fluorescent Ferrous Ion Assay Kit with RhoNox-6 (#S1070S, Beyotime).

Microscopy:

Article Title: TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation.
Article Snippet: .. Propidium Iodide (PI) staining Cells cultured in 24-well plates were stained with PI (#G1021, Servicebio, Wuhan, China) in the dark for 10 min. Stained cells were observed and imaged using a Leica fluorescence microscope. .. The levels of MDA, GSH, and the LIP were measured using commercial assay kits: MDA with the Lipid Peroxidation MDA Assay Kit (#S0131S, Beyotime), GSH with the GSH and GSSG Assay Kit (#S0053, Beyotime), and LIP with the Cellular Red Fluorescent Ferrous Ion Assay Kit with RhoNox-6 (#S1070S, Beyotime).



Similar Products

99
Beyotime crystal violet
Crystal Violet, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/Crystal+Violet+Staining+Solution/pm42909555-54-8-10
Average 99 stars, based on 1 article reviews
crystal violet - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
Beyotime propidium iodide pi staining solution
Propidium Iodide Pi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/Beyo3D+PI+Staining+Solution/pmc13691859-134-14-19
Average 99 stars, based on 1 article reviews
propidium iodide pi staining solution - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
Beyotime 6 diamidino 2 phenylindole
6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/Beyo3D+DAPI+Staining+Solution/pm42908353-49-46-47
Average 99 stars, based on 1 article reviews
6 diamidino 2 phenylindole - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
Beyotime nissl staining solution
Nissl Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/Nissl+Staining+Solution/pm42901416-86-48-51
Average 99 stars, based on 1 article reviews
nissl staining solution - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
Beyotime alizarin red staining
Alizarin Red Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/2%25+Alizarin+Red+S+Staining+Solution%EF%BC%8C+pH4%2E2/pm42897207-554-1-47
Average 99 stars, based on 1 article reviews
alizarin red staining - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

99
Beyotime giemsa staining solution
Giemsa Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/Giemsa+Staining+Solution/pmc13685217-112-7-12
Average 99 stars, based on 1 article reviews
giemsa staining solution - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier

Image Search Results