hematoxylin staining solution  (Servicebio Inc)

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Staining, Expressing, Transmission Assay, Electron Microscopy, Microscopy, Control

Journal: Redox Biology

Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

doi: 10.1016/j.redox.2026.104198

Figure Lengend Snippet: Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

Techniques: Expressing, Staining, Transmission Assay, Electron Microscopy, Microscopy, Control

Journal: Genes & Diseases

Article Title: Non-canonical role of “S6K1–SGK1” pathway in neuronal necroptosis following traumatic brain injury

doi: 10.1016/j.gendis.2025.101876

Figure Lengend Snippet: S6K1 inhibition alleviates neuronal necroptosis through SGK1 following TBI in mice. (A, K) Western blotting showed increased expression of p-S6/S6, SGK1, p-MLKL/MLKL, and p-RIP3/RIP3 in the cortex following TBI. These changes were attenuated by S6K1 inhibitor treatment and S6K1 knockdown, as shown by decreased p-MLKL/MLKL and p-RIP3/RIP3 levels compared with the TBI group. (B–E, L, M) Statistical results of Western blotting. (F) Immunohistochemistry staining of p-S6K1 in the right cortex of mouse brain after TBI. Scale bar: 20 μm. (G) Statistical results of immunohistochemistry staining for the increased S6K1 expression. (H) Schematic illustration of protein detection and behavior paradigm following p-S6K1 inhibitor injection. (I) Nissl staining of decreased neurons after TBI, and increased neurons after treatment with S6K1 inhibitor and knockdown compared with TBI. Scale bar: 20 μm. (J) Statistical results of Nissl staining for the numbers of neurons. (N) Enhanced immunofluorescence intensity of p-MLKL in the right cortex of mouse brain after TBI, and decreased immunofluorescence intensity after treatment with S6K1 inhibitor and knockdown compared with TBI. Scale bar: 50 μm. Two-tailed unpaired Student's t -test was used for (G). n = 3 or 4. ∗ P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 versus the Sham group. # P < 0.05 and ## P < 0.01 versus the TBI group.

Article Snippet: The brain sections were dewaxed and stained with Nissl staining solution (Beyotime) for 10 min.

Techniques: Inhibition, Western Blot, Expressing, Knockdown, Immunohistochemistry, Staining, Injection, Immunofluorescence, Two Tailed Test

Journal: Bioactive Materials

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

doi: 10.1016/j.bioactmat.2026.02.051

Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

Techniques: In Vitro, Fluorescence, Staining