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crystal violet  (Beyotime)


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    Structured Review

    Beyotime crystal violet
    Crystal Violet, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 6782 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/Crystal+Violet+Staining+Solution/pmc12414389-202-44-46
    Average 99 stars, based on 6782 article reviews
    crystal violet - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Staining:

    Article Title: MSRB3 promotes gastric cancer progression and immunotherapy resistance by stabilizing GPX4 to inhibit ferroptosis
    Article Snippet: .. Colonies were fixed with methanol for 30 min, stained with 0.1% crystal violet (Beyotime Biotechnology, Shanghai, China) for 10 min, and quantified using ImageJ software. .. Cell proliferation was assessed via a Cell-Light EdU Apollo staining kit (RiboBio, Guangzhou, China).

    Article Title: DPP9 modulates RPA1 stability and temozolomide sensitivity through RFWD3 Nuclear localization in glioma.
    Article Snippet: .. The resulting colonies were fixed, stained with 0.1% crystal violet (Beyotime, Shanghai, China), and counted manually. ..

    Article Title: Engineering potent avian GPR25 agonists using an ostrich CXCL17 gene relic
    Article Snippet: .. The inserts were put into a 24-well plate containing indicated CXCL17 proteins (diluted in serum-free DMEM plus 0.2% BSA, 500 μL/well) and incubated at 37°C for ∼5 h. Thereafter, solution in the inserts was removed and cells on the upper face of the permeable membrane were wiped off using cotton swaps, and cells on the lower face of the permeable membrane were fixed with 4% paraformaldehyde solution, stained with crystal violet staining solution (Beyotime Technology), and observed under a microscope. .. The migrated cells were quantitated using the ImageJ software and the results were expressed as mean ± SD ( n = 3).

    Article Title: Association of PON2 and Its S311C Substitution with Tumor Progression and Its Significance as an Immune-Associated Prognostic Indicator in Clear Cell Renal Cell Carcinoma.
    Article Snippet: .. Then, 400 μL of 0.1% crystal violet solution (Beyotime Biotech Inc., China) was added to the new 24-well plate, and the chambers were placed into the wells for staining for 8 min. ..

    Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes.
    Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs).

    Crystal Violet Assay:

    Article Title: DIA Quantitative Proteomic Analysis Reveals Early Proteomic Responses to Honeybee-Derived Melittin in Murine Breast Cancer Cells.
    Article Snippet: RIPA lysis buffer, protease inhibitor cocktail, and phosphatase inhibitors A and B were supplied by Epizyme Biotech (Shanghai, China). .. The 4% paraformaldehyde fixative, crystal violet staining solution from Beyotime Biotechnology (Shanghai, China). ..

    Article Title: Disporum cantoniense suppresses non‑small cell lung cancer through AMPK/mTOR/ULK1‑mediated autophagy‑dependent apoptosis.
    Article Snippet: Rapamycin (Rap; cat. no. T1537), compound C (CC; cat. no. T6146), gefitinib (Gefi; cat. no. T1181), ULK101 (cat. no. T5403), Z‐VAD‐FMK (ZVF; cat. no. T7020) and SCH772984 (SCH; cat. no. T6066) were obtained from TargetMol Chemicals Inc. .. Crystal violet staining solution (cat. no. C0121) and GreenNuc Live Cell Caspase‐3 Activity Detection Kit (cat. no. C1168M) were obtained from Beyotime Biotechnology. .. The Lipomaster 3000 Transfection Reagent (cat. no. TL301‐02) was obtained from Vazyme Biotech Co., Ltd.

    Incubation:

    Article Title: Engineering potent avian GPR25 agonists using an ostrich CXCL17 gene relic
    Article Snippet: .. The inserts were put into a 24-well plate containing indicated CXCL17 proteins (diluted in serum-free DMEM plus 0.2% BSA, 500 μL/well) and incubated at 37°C for ∼5 h. Thereafter, solution in the inserts was removed and cells on the upper face of the permeable membrane were wiped off using cotton swaps, and cells on the lower face of the permeable membrane were fixed with 4% paraformaldehyde solution, stained with crystal violet staining solution (Beyotime Technology), and observed under a microscope. .. The migrated cells were quantitated using the ImageJ software and the results were expressed as mean ± SD ( n = 3).

    Article Title: Ectopic endometrial stromal cells‐derived exosomes accelerate endometriosis progression by delivering PDLIM3 to induce macrophage M2 polarization
    Article Snippet: .. Twenty‐eight hours after incubation, discarding the unmigrated cells, fixing the migrated cells, and the dying the migrated cells with crystal violet (Beyotime Biotechnology). .. Finally, the stained cells were observed and captured using an Olympus microscope (Olympus) at 5 random fields.

    Membrane:

    Article Title: Engineering potent avian GPR25 agonists using an ostrich CXCL17 gene relic
    Article Snippet: .. The inserts were put into a 24-well plate containing indicated CXCL17 proteins (diluted in serum-free DMEM plus 0.2% BSA, 500 μL/well) and incubated at 37°C for ∼5 h. Thereafter, solution in the inserts was removed and cells on the upper face of the permeable membrane were wiped off using cotton swaps, and cells on the lower face of the permeable membrane were fixed with 4% paraformaldehyde solution, stained with crystal violet staining solution (Beyotime Technology), and observed under a microscope. .. The migrated cells were quantitated using the ImageJ software and the results were expressed as mean ± SD ( n = 3).

    Microscopy:

    Article Title: Engineering potent avian GPR25 agonists using an ostrich CXCL17 gene relic
    Article Snippet: .. The inserts were put into a 24-well plate containing indicated CXCL17 proteins (diluted in serum-free DMEM plus 0.2% BSA, 500 μL/well) and incubated at 37°C for ∼5 h. Thereafter, solution in the inserts was removed and cells on the upper face of the permeable membrane were wiped off using cotton swaps, and cells on the lower face of the permeable membrane were fixed with 4% paraformaldehyde solution, stained with crystal violet staining solution (Beyotime Technology), and observed under a microscope. .. The migrated cells were quantitated using the ImageJ software and the results were expressed as mean ± SD ( n = 3).

    Activity Assay:

    Article Title: Disporum cantoniense suppresses non‑small cell lung cancer through AMPK/mTOR/ULK1‑mediated autophagy‑dependent apoptosis.
    Article Snippet: Rapamycin (Rap; cat. no. T1537), compound C (CC; cat. no. T6146), gefitinib (Gefi; cat. no. T1181), ULK101 (cat. no. T5403), Z‐VAD‐FMK (ZVF; cat. no. T7020) and SCH772984 (SCH; cat. no. T6066) were obtained from TargetMol Chemicals Inc. .. Crystal violet staining solution (cat. no. C0121) and GreenNuc Live Cell Caspase‐3 Activity Detection Kit (cat. no. C1168M) were obtained from Beyotime Biotechnology. .. The Lipomaster 3000 Transfection Reagent (cat. no. TL301‐02) was obtained from Vazyme Biotech Co., Ltd.



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