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trypan blue stain solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypan blue stain solution
    Trypan Blue Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/Trypan+Blue/pm42334629-65-11-16
    Average 99 stars, based on 1 article reviews
    trypan blue stain solution - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Random Hexamer:

    Article Title: Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells
    Article Snippet: Annexin V FLUOS was purchased from Roche Diagnostics GmbH (Mannheim, Germany). .. Calcein-AM, dNTP mix (10 mmol/l), Random Hexamer Primer (0.2 μg/μl), Reaction Buffer (5X), ReverAid H minus Reverse Transcriptase, RiboLock RNase Inhibitor, SYBr Green I Nucleic Gel Stain, Trypan blue, Ultrapure water were purchased from Invitrogen via Thermo Fisher Scientific (Waltham, MA, USA). ..

    Reverse Transcription:

    Article Title: Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells
    Article Snippet: Annexin V FLUOS was purchased from Roche Diagnostics GmbH (Mannheim, Germany). .. Calcein-AM, dNTP mix (10 mmol/l), Random Hexamer Primer (0.2 μg/μl), Reaction Buffer (5X), ReverAid H minus Reverse Transcriptase, RiboLock RNase Inhibitor, SYBr Green I Nucleic Gel Stain, Trypan blue, Ultrapure water were purchased from Invitrogen via Thermo Fisher Scientific (Waltham, MA, USA). ..

    SYBR Green Assay:

    Article Title: Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells
    Article Snippet: Annexin V FLUOS was purchased from Roche Diagnostics GmbH (Mannheim, Germany). .. Calcein-AM, dNTP mix (10 mmol/l), Random Hexamer Primer (0.2 μg/μl), Reaction Buffer (5X), ReverAid H minus Reverse Transcriptase, RiboLock RNase Inhibitor, SYBr Green I Nucleic Gel Stain, Trypan blue, Ultrapure water were purchased from Invitrogen via Thermo Fisher Scientific (Waltham, MA, USA). ..

    Staining:

    Article Title: Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells
    Article Snippet: Annexin V FLUOS was purchased from Roche Diagnostics GmbH (Mannheim, Germany). .. Calcein-AM, dNTP mix (10 mmol/l), Random Hexamer Primer (0.2 μg/μl), Reaction Buffer (5X), ReverAid H minus Reverse Transcriptase, RiboLock RNase Inhibitor, SYBr Green I Nucleic Gel Stain, Trypan blue, Ultrapure water were purchased from Invitrogen via Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: Reduced serum interleukin-2 associates with higher motor severity and along with CD4 T cell alterations may be an early event in isolated REM Sleep Behaviour Disorder
    Article Snippet: .. Wash buffer was discarded, and cells were resuspended in 10 mL of PBS containing 2% FBS, and the cell count was determined using 0.4% trypan blue stain (Thermo Fisher) and a Countess automated cell counter (Thermo Fisher). ..

    Article Title: Gravitational and mechanical forces shape mitochondrial translation.
    Article Snippet: .. Then, cells were stained with trypan blue, and live cells were quantified with Countess (Thermo Fisher Scientific). .. Mitochondrial ATP production was monitored with Mitochondrial ToxGlo Assay (Promega) according to themanufacturer’s instructions.

    Cell Characterization:

    Article Title: Reduced serum interleukin-2 associates with higher motor severity and along with CD4 T cell alterations may be an early event in isolated REM Sleep Behaviour Disorder
    Article Snippet: .. Wash buffer was discarded, and cells were resuspended in 10 mL of PBS containing 2% FBS, and the cell count was determined using 0.4% trypan blue stain (Thermo Fisher) and a Countess automated cell counter (Thermo Fisher). ..

    Suspension:

    Article Title: Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.
    Article Snippet: Accurate and reproducible collection of the draining lymph nodes (DLNs) is essential for studying immune responses in preclinical models, yet technical variability and the small size of murine lymph nodes make consistent harvest challenging, particularly across users with differing surgical experience.. To address this limitation, we adapted the clinical technique of isosulfan blue sentinel lymph node biopsy (IB-SLNB) for use in mice.. Despite its widespread application in surgical oncology, IB has not been systemically evaluated for compatibility with fluorescence-based immunologic assays.

    other:

    Article Title: Chimeric switch scaffold protein augments CAR synapse formation and signaling networks.
    Article Snippet: Cells were counted every 4 days using a Countess II Automated Cell Counter (Thermo Fisher Scientific), with trypan blue (Gibco) for viability exclusion.

    Incubation:

    Article Title: Investigation of Intracellular Clearing of Streptococcus pneumoniae by mRNA-Encoded Cpl-1 Bacteriophage Endolysin in Human Macrophages.
    Article Snippet: For surface antibody staining, cells were incubated with 5 μL Monocyte Blocker and 5 μL TruStain FcX (BioLegend) for 5–10 min at room temperature, followed by staining with APC anti-human CD11b (BioLegend) (1.5 μg/mL) for 10–15 min. .. For trypan blue quenching, samples were resuspended in 250 μL PBS+ EDTA and incubated with 1.2 mg/mL trypan blue (Gibco® Life Technologies, Darmstadt, Germany) for 5 min before being washed twice with PBS+ EDTA and resuspended in 80 μL PBS + EDTA. .. Cells were washed twice in Perm Wash Buffer (BioLegend) and finally resuspended in PBS + EDTA for analysis.



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    Servicebio Inc ros staining solution
    Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans <t>blue</t> <t>staining</t> to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of <t>ROS</t> and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.
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    Image Search Results


    Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

    Journal: Redox Biology

    Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

    doi: 10.1016/j.redox.2026.104198

    Figure Lengend Snippet: Assessment of the improvement effect of PG on MIRI. A: Chemical structural formula of PG; B-D used echocardiography to measure LVEF and LVFS in the mouse; E-F: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; G-H: Serum cTnI and CK-MB expression levels; I-J: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); K: Serum LDH expression level; L: Using the mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Using the HL-1 cell OGD/R model to observe the effect of PG on lactate levels; N: Using the mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using the HL-1 cell OGD/R model to observe the effect of PG on ATP levels; P: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); Q: Quantitative analysis of transmission electron microscope images; Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R.

    Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

    Techniques: Staining, Expressing, Transmission Assay, Electron Microscopy, Microscopy, Control

    Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

    Journal: Redox Biology

    Article Title: Piceatannol-3′-O-β-d-glucopyranoside mitigates myocardial ischemia-reperfusion injury by inhibiting ferroptosis through the regulation of NDUFS1 lactylation via metabolic reprogramming

    doi: 10.1016/j.redox.2026.104198

    Figure Lengend Snippet: Observing whether exogenous lactate supplementation attenuates PG's effect on improving MIRI. A-C: Detect LVEF and LVFS in the mouse using echocardiography; D-E: Serum cTnI and CK-MB expression levels; F-G: TTC/Evans blue staining to detect myocardial infarction area in mouse hearts; H–I: DCFH-DA detection of ROS and their expression levels in mouse myocardial tissue (scale bar = 20 μm); J: Structure of cardiac mitochondria observed by transmission electron microscopy (scale bar = 500 nm); K: Quantitative analysis of transmission electron microscope images; L: Establish a mouse I/R model to observe the effect of PG on lactate levels in cardiac tissue; M: Serum LDH expression level; N: Establish a mouse I/R model to observe the effect of PG on cardiac tissue ATP levels; O: Using HL-1 cells to establish an OGD/R model to observe the effect of PG on lactate levels. Data were expressed as mean ± SD (n ≥ 3), ## P < 0.01, ### P < 0.001, #### P < 0.0001 VS Sham or Control; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 VS I/R or OGD/R; & P < 0.05, && P < 0.01, &&& P < 0.001, VS I/R + PG or OGD/R + PG.

    Article Snippet: Incubate at room temperature for 5 min, then rinse with pure water for 10 min. Then add ROS staining solution (G1746, Servicebio) dropwise, incubate at 37 °C in a dark incubator for 30 min, and wash three times with PBS for 5 min each.

    Techniques: Expressing, Staining, Transmission Assay, Electron Microscopy, Microscopy, Control