jc 1 staining solution (Beyotime)

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Beyotime jc 1 staining solution
Jc 1 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 15061 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution+mix+detection+kit/JC-1/pmc07485299-82-0-9
Average 99 stars, based on 15061 article reviews

jc 1 staining solution - by Bioz Stars, 2026-09

99/100 stars

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Membrane:

Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
Article Snippet: Intracellular reactive oxygen species levels were measured using the DCFH-DA probe (MA0219, MeilunBio, China). .. Changes in mitochondrial membrane potential were assessed by JC-1 staining (C2003S, Beyotime). ..

Article Title: Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca 2+ signal during respiratory motion
Article Snippet: Intracellular reactive oxygen species (ROS) levels were assessed using a ROS detection kit (S0033S, Beyotime, China). .. Mitochondrial membrane potential was evaluated using the JC-1 kit (C2006, Beyotime, China). ..

Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development
Article Snippet: .. After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope. ..

Article Title: ROS-responsive hydrogels functionalized with Cu/Zn MOF targeting oxidative stress mitigation and inflammation modulation to promote spinal cord injury repair
Article Snippet: Zinc nitrate hexahydrate (98%, Zn(NO 3 ) 2 ·6H 2 O), copper nitrate trihydrate (98%, Cu(NO 3 ) 2 ·3H 2 O), 2-Methylimidazole (99%), L-glutathione reduced (≥98.0%), chitosan solution (QCS), tannic acid (TA), sodium bicarbonate (NaHCO 3 ), lipopolysaccharide (LPS), Methylene blue, Rhodamine B, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Collagenase type I, and 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) were purchased from Sigma Aldrich Co. Ltd. Methanol, hydrogen peroxide, and ethanol were purchased from Macklin. .. Dichlorodihydrofluorescein diacetate kit (DCFH-DA), Dihydroethidium kit (DHE), CCK-8 Kit, Calcein/PI cell viability/cytotoxicity assay kit, Nitroblue tetrazolium (NBT), Enhanced mitochondrial membrane potential assay kit with JC-1, Mitochondrial Superoxide Assay Kit with MitoSOTM Red, and Luxol fast blue (LFB) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. Dulbecco's modified eagle medium (DMEM), Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12), Poly-D-lysine (PDL), Neurobasal-A medium (NB), B-27 Serum-Free Supplement (B-27), Hank's balanced salt solution (HBSS), Trypsin, fetal bovine serum (FBS) and phosphate buffer solution (PBS) were obtained from Gibco. ..

Article Title: Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy
Article Snippet: .. In mitochondrial levels, to investigate the mitochondrial membrane potential (MMP), the treated cells were rinsed with PBS for 3 times, and added with 5 μg/mL JC-1 working solution (Beyotime, China). ..

Staining:

Incubation:

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
Article Snippet: .. After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm).

ROS Assay:

Fluorescence:

Microscopy:

CCK-8 Assay:

Modification:

Multiple Displacement Amplification:

Article Title: Multifunctional armored nanoemulsion of elemene combining ferroptosis induction and gut homeostasis restoration in colorectal cancer therapy
Article Snippet: D-Luciferin potassium salt, and fluorescent 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine-4-chlorobenzene sulfonate (DiD), coumarin-6 (C6), and Liproxstatin-1 (Lip-1, purity >99%) were purchased from Sigma Aldrich. .. CM-H2DCFDA kit, BODIPY 581/591 C11kit, JC-1 kit, MDA kit, GSH kit, GSH-PX kit, mouse TNF-α kit, and mouse IFN-γ ELISA kit were purchased from Beyotime Biotechnology, Shanghai, China. ..

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